LOCUS       sequence-51913-e         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: FKBP14
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             25..42
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             67..87
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             522..539
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(592..610)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      606..653
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(777..796)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctttaagaag gagatataca tatgcaccat catcatcatc attcttctgg tgtagatctg
       61 ggtaccgaga acctgtactt ccaatccatg ggggctttga tccctgaacc agaagtgaaa
      121 attgaagttc tccagaagcc attcatctgc catcgcaaga ccaaaggagg ggatttgatg
      181 ttggtccact atgaaggcta cttagaaaag gacggctcct tatttcactc cactcacaaa
      241 cataacaatg gtcagcccat ttggtttacc ctgggcatcc tggaggctct caaaggttgg
      301 gaccagggct tgaaaggaat gtgtgtagga gagaagagaa agctcatcat tcctcctgct
      361 ctgggctatg gaaaagaagg aaaaggtaaa attcccccag aaagtacact gatatttaat
      421 attgatctcc tggagattcg aaatggacca agatcctgac agtaaaggtg gatacggatc
      481 cgaattcgag ctccgtcgac aagcttgcgg ccgcactcga gcaccaccac caccaccact
      541 gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc
      601 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag
      661 gaggaactat atccggattg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
      721 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
      781 tttcgctttc ttcccttcct ttc
//
