LOCUS       sequence-51920-e         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: C14orf169
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(96..119)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(120..137)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(138..158)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 tttcgggctt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agtgcggccg
       61 caagcttgtc gacggagctc gaattcggat cctcacttat cgtcgtcatc cttgtaatca
      121 tggtggtgat gatggtgcga ttggaagtag aggttctctg catttagggc tagaggcatc
      181 ttagtgagca gcagcccctt atcatacaac gtggttgcca aggacagctg gtcctccaca
      241 ctgtcacagg gcaggtcccc cactctcaca aactctggat aagaaccaag caacagttcc
      301 atggcatcag cttgctgggg gtatatttcc aagcacttgg gttcttccag atgatacaca
      361 cgggagtttt ccactgtgta atagagaaac aaatggcccc cctcacccac cagccgagct
      421 atcccatcct gaagcatatg gacttctgtt tctgttgtca actgggcccc cacgtttaca
      481 ggttctccag cctcccagcg aattggaagc ccgtaaacac ttagtgccct ctccctatca
      541 gtcaaaacag ggggcagaga atcgtgaatg aagtctttgg ctcgctggtc ggccacagca
      601 tcaacaggag caaagtgtcc caggcgggca accaagaccc gcaccttctc catgaaagcg
      661 gttcttcgcg gatccttaga atctgaatgc tgggccccca tgtaatccat gaagtctcgg
      721 ggcagacccc tccgaaactc cacattttct tccattgcag cctgcactgc cagaggcagt
      781 atggcctcta agaagtcacc ccaggtattg cgctggtacg tggacaaggt gaggtgcaga
      841 gagtggactc catcctggca ttcagcttgg tgaatgaagc cc
//
