LOCUS       sequence-51923-e         679 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 679)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: TAF1
FEATURES             Location/Qualifiers
     source          1..679
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(490..510)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(535..552)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             563..585
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    600..624
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(624..643)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(625..643)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggtggtggtg gtggtgctcg agtgcggccg caagcttgtc gacggagctc gaattcggat
       61 ccgtatccac ctttactgtc aatccaggct ttccagttcc gcttcttcca gcgccgcttc
      121 tttggcggtg cagatatctt tttccagctg ggtcagatgt tcatcatatt cggtcagggt
      181 ctgataacaa acgttcacaa tttcctgcgc ggttttggta tactggcttt ccggaccatt
      241 atatttaacg ctattggcca gaatcagatt cacatcatcc agaaagcttt cgcggctctg
      301 atatttgtgt ttgctaatat ttttacgaat ggtttccaga tccatcgggt taacaatcac
      361 tttataataa tccggcacaa attttttgtt caccgggtga tgaaacggcc agctatccgg
      421 cacggccatc attttctggg tcacaatgtt atccagaatg aagctaaagg ccacctgatc
      481 atcatccatg gattggaagt acaggttctc ggtacccaga tctacaccag aagaatgatg
      541 atgatgatgg tgcatatgta tatctccttc ttaaagttaa acaaaattat ttctagaggg
      601 gaattgttat ccgctcacaa ttcccctata gtgagtcgta ttaatttcgc gggatcgaga
      661 tctcgatcct ctacgccgg
//
