LOCUS       sequence-51943-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Hammond GR, Fischer MJ, Anderson KE, Holdich J, Koteci A, Balla T, 
            Irvine RF
  TITLE     PI4P and PI(4,5)P2 Are Essential But Independent Lipid Determinants 
            of Membrane Identity.
  JOURNAL   Science. 2012 Jun 21.
  PUBMED    22722250
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2012 Jun 21."
COMMENT     Sequence Label: LYN11-FRB-CFP
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(81..98)
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
     CDS             113..391
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISK"
     CDS             398..424
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(482..503)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(743..762)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ttagtgaacc gtcagatccg ctagcaccat gggatgtata aaatcaaaag ggaaagacag
       61 cagatctgcg aattcaggtg ctggtgctgg tgctggtgct atcctatcta gaatcctctg
      121 gcatgagatg tggcatgaag gcctggaaga ggcatctcgt ttgtactttg gggaaaggaa
      181 cgtgaaaggc atgtttgagg tgctggagcc cttgcatgct atgatggaac ggggccccca
      241 gactctgaag gaaacatcct ttaatcaggc ctatggtcga gatttaatgg aggcccaaga
      301 gtggtgcagg aagtacatga aatcagggaa tgtcaaggac ctcacccaag cctgggacct
      361 ctattatcat gtgttccgac gaatctcaaa gactagttat ccgtacgacg taccagacta
      421 cgcaccggtc gccaccctgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
      481 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
      541 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
      601 cgtgccctgg cccaccctcg tgaccaccct gacctggggc gtgcagtgct tcagccgcta
      661 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
      721 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
      781 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
//
