LOCUS       sequence-51949-e         438 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Lim KH, Huang H, Pralle A, Park S
  TITLE     Stable, high-affinity streptavidin monomer for protein labeling and 
            monovalent biotin detection.
  JOURNAL   Biotechnol Bioeng. 2013 Jan;110(1):57-67. doi: 10.1002/bit.24605. 
            Epub 2012 Aug 8.
  PUBMED    22806584
REFERENCE   2  (bases 1 to 438)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.24605";
            journalName: "Biotechnol Bioeng"; date: "2013-01"; volume: "110"; 
            issue: "1"; pages: "57-67"
COMMENT     Sequence Label: pRSET-mSA
FEATURES             Location/Qualifiers
     source          1..438
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..411
                     /codon_start=1
                     /product="monomeric streptavidin/rhizavidin hybrid (Lim et 
                     al., 2013)"
                     /label=mSA
                     /translation="AEAGITGTWYNQSGSTFTVTAGADGNLTGQYENRAQGTGCQNSPY
                     TLTGRYNGTKLEWRVEWNNSTENCHSRTEWRGQYQGGAEARINTQWNLTYEGGSGPATE
                     QGQDTFTKVK"
ORIGIN
        1 actttaagaa ggagatatac catgggcagc agccatcacc atcatcacca cagccaggat
       61 ctggctagcg cggaagcggg tatcaccggc acgtggtaca accagtctgg ttctaccttc
      121 accgttaccg cgggtgcgga cggtaacctg accggtcagt acgaaaaccg tgcgcagggc
      181 actggttgcc agaactctcc gtacaccctg accggtcgtt acaacggtac caaactggaa
      241 tggcgtgttg aatggaacaa ctctaccgaa aactgccact ctcgtaccga atggcgtggt
      301 cagtaccagg gtggtgcgga agcgcgtatc aacacccagt ggaacctgac ctacgaaggt
      361 ggttctggtc cggcgaccga acagggtcag gacaccttca ccaaagttaa accgtctgcg
      421 gcgtctggat ccgactac
//
