LOCUS       sequence-51951-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Lim KH, Huang H, Pralle A, Park S
  TITLE     Stable, high-affinity streptavidin monomer for protein labeling and 
            monovalent biotin detection.
  JOURNAL   Biotechnol Bioeng. 2013 Jan;110(1):57-67. doi: 10.1002/bit.24605. 
            Epub 2012 Aug 8.
  PUBMED    22806584
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.24605";
            journalName: "Biotechnol Bioeng"; date: "2013-01"; volume: "110"; 
            issue: "1"; pages: "57-67"
COMMENT     Sequence Label: pRSET-mSA-EGFP
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(34..57)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(79..420)
                     /codon_start=1
                     /product="monomeric streptavidin/rhizavidin hybrid (Lim et 
                     al., 2013)"
                     /label=mSA
                     /translation="AEAGITGTWYNQSGSTFTVTAGADGNLTGQYENRAQGTGCQNSPY
                     TLTGRYNGTKLEWRVEWNNSTENCHSRTEWRGQYQGGAEARINTQWNLTYEGGSGPATE
                     QGQDTFTKVK"
     CDS             complement(439..456)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             475..497
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(528..547)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(529..547)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(632..649)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctcgcccttg ctcaccatgc taccgccctc gagcttgtcg tcatcgtcct tgtagtcgga
       61 tccagacgcc gcagacggtt taactttggt gaaggtgtcc tgaccctgtt cggtcgccgg
      121 accagaacca ccttcgtagg tcaggttcca ctgggtgttg atacgcgctt ccgcaccacc
      181 ctggtactga ccacgccatt cggtacgaga gtggcagttt tcggtagagt tgttccattc
      241 aacacgccat tccagtttgg taccgttgta acgaccggtc agggtgtacg gagagttctg
      301 gcaaccagtg ccctgcgcac ggttttcgta ctgaccggtc aggttaccgt ccgcacccgc
      361 ggtaacggtg aaggtagaac cagactggtt gtaccacgtg ccggtgatac ccgcttccgc
      421 gctagccaga tcctggctgt ggtgatgatg gtgatggctg ctgcccatgg tatatctcct
      481 tcttaaagtt aaacaaaatt atttctagag ggaaaccgtt gtggtctccc tatagtgagt
      541 cgtattaatt tcgcgggatc gagatcctgc attaatgaat cggccaacgc gcggggagag
      601 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
      661 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
      721 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
      781 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
//
