LOCUS       sequence-51955-e         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Lim KH, Huang H, Pralle A, Park S
  TITLE     Stable, high-affinity streptavidin monomer for protein labeling and 
            monovalent biotin detection.
  JOURNAL   Biotechnol Bioeng. 2013 Jan;110(1):57-67. doi: 10.1002/bit.24605. 
            Epub 2012 Aug 8.
  PUBMED    22806584
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.24605";
            journalName: "Biotechnol Bioeng"; date: "2013-01"; volume: "110"; 
            issue: "1"; pages: "57-67"
COMMENT     Sequence Label: pYD1-mSA
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(333..356)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(378..719)
                     /codon_start=1
                     /product="monomeric streptavidin/rhizavidin hybrid (Lim et 
                     al., 2013)"
                     /label=mSA
                     /translation="AEAGITGTWYNQSGSTFTVTAGADGNLTGQYENRAQGTGCQNSPY
                     TLTGRYNGTKLEWRVEWNNSTENCHSRTEWRGQYQGGAEARINTQWNLTYEGGSGPATE
                     QGQDTFTKVK"
     CDS             complement(783..809)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gggcgaattg gagctcaatt ctcttaggat tcgattcaca ttcatctttt tttagctact
       61 aataggataa attataggaa tttataacaa attgaaatat ggcaggcagc aaaattaaaa
      121 ttgtcttagt tttttagtgt atagaagtga atagctatat aaagtatgtg taaagttggt
      181 aacggaacga aaaatagaaa aggatattac atgggaaaac atgttgttta cggagaaatg
      241 aaaagtatat tgtattttgt acgagctaaa agtacagtgg gaacaaagtc gattttgtta
      301 catctacact gttgttatca gatctcgagt tacttgtcgt catcgtcctt gtagtcggat
      361 ccagacgccg cagacggttt aactttggtg aaggtgtcct gaccctgttc ggtcgccgga
      421 ccagaaccac cttcgtaggt caggttccac tgggtgttga tacgcgcttc cgcaccaccc
      481 tggtactgac cacgccattc ggtacgagag tggcagtttt cggtagagtt gttccattca
      541 acacgccatt ccagtttggt accgttgtaa cgaccggtca gggtgtacgg agagttctgg
      601 caaccagtgc cctgcgcacg gttttcgtac tgaccggtca ggttaccgtc cgcacccgcg
      661 gtaacggtga aggtagaacc agactggttg taccacgtgc cggtgatacc cgcttccgcg
      721 ctagcagaac caccaccacc agaaccacca ccaccagaac caccaccacc actagcctgc
      781 agagcgtagt ctggaacgtc gtatgggtat ctacc
//
