LOCUS       sequence-51956-e         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Lakkaraju AK, Mary C, Scherrer A, Johnson AE, Strub K
  TITLE     SRP keeps polypeptides translocation-competent by slowing 
            translation to match limiting ER-targeting sites.
  JOURNAL   Cell. 2008 May 2;133(3):440-51.
  PUBMED    18455985
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2008-05-2"; volume: "133(3)"; pages: "440-51"
COMMENT     Sequence Label: pGFH-14c
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             292..300
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    591..712
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(628..647)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     682..701
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 agtccggact cagatctcga gctcaagctt cgaattctat ggtgttgttg gagagcgagc
       61 agttcctgac ggagctgacc agacttttcc agaagtgccg gacgtcgggc agcgtctata
      121 tcaccttgaa gaagtatgac ggtcgaacca aacccattcc aaagaaaggt actgtggagg
      181 gctttgagcc cgcagacaac aagtgtctgt taagagctac cgatgggaag aagaagatca
      241 gcactgtggt gagctccaag gaagtgaata agtttcagat ggcttattca aacctcctta
      301 gagctaacat ggatgggttg aagaagagag acaaaaagaa caaaactaag aagaccaaag
      361 cagcagcagc agcagcagca gcagcacctg ccgcagcagc aacagcagca acaacagcag
      421 caacaacagc agcaacagca gcacagtaaa gggcataccc gggatccacc ggatctagat
      481 aactgatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
      541 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
      601 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
      661 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct taacgcgtaa
      721 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
      781 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
      841 gggttgagtg ttgttccagt ttg
//
