LOCUS       sequence-51961-e         678 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Griffin EE, Odde DJ, Seydoux G
  TITLE     Regulation of the MEX-5 gradient by a spatially segregated 
            kinase/phosphatase cycle.
  JOURNAL   Cell. 2011 Sep 16;146(6):955-68.
  PUBMED    21925318
REFERENCE   2  (bases 1 to 678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-09-16"; volume: "146(6)"; pages: "955-68"
COMMENT     Sequence Label: pEG196
FEATURES             Location/Qualifiers
     source          1..678
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             30..41
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    45..69
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 acacacataa caataacaac aacctcggga tcgagggaag gatcacaagt ttgtacaaaa
       61 aagcaggctg gatgaaagcg gcatcaaata gtgtctcgtc ggccggagga tcagtgtcac
      121 ctacgacaac ccagccacca ctaccaccag ggcagtcgtc acatcctcaa atctacgatc
      181 aacagatgca gtactacttc gcggctgcca tgccgaacca gccaatggcg acgtatgcgg
      241 cacaaaatgg atcttctcaa cagtacgccc cagcggctcc atattatcaa gatgccaacg
      301 gacagtatgt gcaggttccc gcgaacggat caatggctcc tcaacagcac atgatggttt
      361 ctggtcaacc gtatctctat atggctcaac cacaacaagg agctcagcaa gtcatgcagt
      421 caggacaacc acagctcatc tattatcaac aatcaatggc tccacaagcg gctccaatgt
      481 acttccatcc tatgcaagcc gcaccgatgc ttccagaaca aatgggtgtg atgccacata
      541 cgcagccggc aattccacct caacaacagc caagacaagt gggagtggag attagtagca
      601 cacgcacagc tccattgacc tcgtctactc ctcttccaac aagtctcgaa tacgagactg
      661 ttcagcgtga taatcgca
//
