LOCUS       sequence-51966-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Adamson DN, Lim HN
  TITLE     Rapid and robust signaling in the CsrA cascade via RNA-protein 
            interactions and feedback regulation.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 
            10.1073/pnas.1308476110. Epub 2013 Jul 22.
  PUBMED    23878244
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1308476110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-08-6- 6"; volume: "110"; issue: "32"; pages: "13120-5"
COMMENT     Sequence Label: pHL661
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      42..128
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    183..199
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(371..389)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     639..658
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 actatacaaa taagggccca tggtacgcgt gctagaggca tcaaataaaa cgaaaggctc
       61 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta
      121 ggacaaatcc gccgccctag acctaggcgc ggatccataa atgtgagcgg ataacattga
      181 cattgtgagc ggataacaag atactcccgg taaggaggaa aaaaaatggt gagcaagggc
      241 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
      301 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
      361 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
      421 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
      481 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
      541 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
      601 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
      661 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
      721 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
      781 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaa
//
