LOCUS       sequence-51970-e         650 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Motegi F, Zonies S, Hao Y, Cuenca AA, Griffin E, Seydoux G
  TITLE     Microtubules induce self-organization of polarized PAR domains in 
            Caenorhabditis elegans zygotes.
  JOURNAL   Nat Cell Biol. 2011 Oct 9;13(11):1361-7. doi: 10.1038/ncb2354.
  PUBMED    21983565
REFERENCE   2  (bases 1 to 650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Cell Biol"; date: "2011-10-9- 9"; volume: "13"; issue: "11"; 
            pages: "1361-7"
COMMENT     Sequence Label: pFM032
FEATURES             Location/Qualifiers
     source          1..650
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             315..335
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             348..392
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 ttgttggagt aagtttaaac ttggacttac taactaacgg attatattta aattttcaga
       61 acattaacat ggcactacta ctagaaggtg gaggtcacta cctttgcgat tttaaaacta
      121 cttacaaagc aaagaaggtc gtccaacttc cagatgcaca ctttgttgat cacagaattg
      181 aaatactagg aaacgattcg gattacaaca aagttaagct atacgaacac gcagttgcaa
      241 gatacagtcc tctaccaagt caagcatggt gcccggggga tcggtggagc tccaccggtg
      301 gcggccgctc gagagagaat ctttattttc agggcgccgc caaattcaaa gaaaccgctg
      361 ctgctaaatt cgaacgccag cacatggaca gcggaggtgg aggtactagt ggatcctaat
      421 tttgccgtat tttccatatt ttgttttgta tatttatcca ctcaccccct ctctttgtcc
      481 tgtgaatgaa cttgtgccaa aaaagccaaa aataatttac tttttttaaa gacatattaa
      541 aacttgaaaa cttggaacaa tacggtgcga aagcgaaaca gagacagaaa aatgaacaaa
      601 gagatccgtg aaaagtgaac gatgatttag gcttcttgtt gggcggcgtg
//
