LOCUS       sequence-51974-e         657 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Griffin EE, Odde DJ, Seydoux G
  TITLE     Regulation of the MEX-5 gradient by a spatially segregated 
            kinase/phosphatase cycle.
  JOURNAL   Cell. 2011 Sep 16;146(6):955-68.
  PUBMED    21925318
REFERENCE   2  (bases 1 to 657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-09-16"; volume: "146(6)"; pages: "955-68"
COMMENT     Sequence Label: pEG408
FEATURES             Location/Qualifiers
     source          1..657
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(409..453)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(466..486)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ttaggatcca ctagtctaat agtgtcctct tagtccgaga ttttcctcag atgtccactt
       61 cgtgctgaga gatgtgcgac gcttgttcat cggtggattt tctctgcgat tccgtctcaa
      121 gtgtggtggc aagtcgtctt cgttggattc cggttgattg taatcacctc cggcttgaag
      181 atcgcgacca ncattggaga ggcacatcat tgagcctgcc acatttcgat gtttgagcat
      241 cactttggtc ggcgtggtag ctcttccacc tgtacgcatg aatcgtggct gatgtctagc
      301 gacaacgtag tcttctggaa tgacatcctg gtcgtaatcc tggtcatcgt gagacatgcg
      361 ctgatatgga ggaatattct ggaattcctt actagtacct ccacctccgc tgtccatgtg
      421 ctggcgttcg aatttagcag cagcggtttc tttgaatttg gcggcgccct gaaaataaag
      481 attctctctc gagcggccgc caccggtgga gctccaccga tcccccgggc accatgcttg
      541 acttggtaga ggactgtatc ttgcaactgc gtgttcgtat agcttaactt tgttgtaatc
      601 cgaatcgttt cctagtattt caattctgtg atcaacaaag tgtgcatctg gaagttg
//
