LOCUS       sequence-51977-e         723 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Griffin EE, Odde DJ, Seydoux G
  TITLE     Regulation of the MEX-5 gradient by a spatially segregated 
            kinase/phosphatase cycle.
  JOURNAL   Cell. 2011 Sep 16;146(6):955-68.
  PUBMED    21925318
REFERENCE   2  (bases 1 to 723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-09-16"; volume: "146(6)"; pages: "955-68"
COMMENT     Sequence Label: pEG412
FEATURES             Location/Qualifiers
     source          1..723
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             246..266
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             279..323
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 tggcactact actagaaggt ggaggtcact acctttgcga ttttaaaact acttacaaag
       61 caaagaaggt cgtccaactt ccagatgcac actttgttga tcacagaatt gaaatactag
      121 gaaacgattc ggattacaac aaagttaagc tatacgaaca cgcagttgca agatacagtc
      181 ctctaccaag tcaagcatgg tgcccggggg atcggtggag ctccaccggt ggcggccgct
      241 cgagagagaa tctttatttt cagggcgccg ccaaattcaa agaaaccgct gctgctaaat
      301 tcgaacgcca gcacatggac agcggaggtg gaggtactag tggatcctaa ttttgccgta
      361 ttttccatat tttgttttgt atatttatcc actcaccccc tctctttgtc ctgtgaatga
      421 acttgtgcca aaaaagccaa aaataattta ctttttttaa agacatatta aaacttgaaa
      481 acttggaaca atacggtgcg aaagcgaaac agagacagaa aaatgaacaa agagatccgt
      541 gaaaagtgaa cgatgattta ggcttcttgt tgggcggcgt ggatgagagt gatgttgtct
      601 cctttgagaa gaattctgcc gatcttgttg cgtcccttcg tcttcatgtt cacctcctcg
      661 gcttcgtcga acacgacatt catgaactcg tcgaatccga tgatgtagcc ctcgagacgg
      721 tgg
//
