LOCUS       sequence-51978-e         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Griffin EE, Odde DJ, Seydoux G
  TITLE     Regulation of the MEX-5 gradient by a spatially segregated 
            kinase/phosphatase cycle.
  JOURNAL   Cell. 2011 Sep 16;146(6):955-68.
  PUBMED    21925318
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-09-16"; volume: "146(6)"; pages: "955-68"
COMMENT     Sequence Label: pEG459
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             24..35
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    39..63
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ataacaataa caacaacctc gggatcgagg gaaggatcac aagtttgtac aaaaaagcag
       61 gcttaatgaa agcggcatca aatagtgtct cgtcggccgg aggatcagtg tcacctacga
      121 caacccagcc accactacca ccagggcagt cgtcacatcc tcaaatctac gatcaacaga
      181 tgcagtacta cttcgcggct gccatgccga accagccaat ggcgacgtat gcggcacaaa
      241 atggatcttc tcaacagtac gccccagcgg ctccatatta tcaagatgcc aacggacagt
      301 atgtgcaggt tcccgcgaac ggatcaatgg ctcctcaaca gcacatgatg gtttctggtc
      361 aaccgtatct ctatatggct caaccacaac aaggagctca gcaagtcatg cagtcaggac
      421 aaccacagct catctattat caacaatcaa tggctccaca agcggctcca atgtacttcc
      481 atcctatgca agccgcaccg atgcttccag aacaaatggg tgtgatgcca catacgcagc
      541 cggcaattcc acctcaacaa cagccaagac aagtgggagt ggagattagt agcacacgca
      601 cagctccatt gacctcgtct actcctcttc caacaagtct cgaatacgag actgttcagc
      661 gtgataatcg caacaggaac atccaattca gatatcatcg tgttatggag catgacgagt
      721 tgccgatcga tgagatctca aagatcaccc ttgacaatca caacgatgac acgatgagcg
      781 ctgaa
//
