LOCUS       sequence-51997-e         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Lakkaraju AK, Mary C, Scherrer A, Johnson AE, Strub K
  TITLE     SRP keeps polypeptides translocation-competent by slowing 
            translation to match limiting ER-targeting sites.
  JOURNAL   Cell. 2008 May 2;133(3):440-51.
  PUBMED    18455985
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2008-05-2"; volume: "133(3)"; pages: "440-51"
COMMENT     Sequence Label: pGFH14A6-12
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             313..321
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    621..742
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(658..677)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     712..731
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 agtccggact cagatctcga gctcaagctt cgaattcacg tcgagccagc gtcgccgcga
       61 tggtgttgtt ggagagcgag cagttcctga cggagctgac cagacttttc cagaagtgcc
      121 ggacgtcggg cagcgtctat atcaccttga agaagtatga cggtcgaacc aaacccattc
      181 caaagaaagg tactgtggag ggctttgagc ccgcagacaa caagtgtctg ttaagagcta
      241 ccgatgggaa gaagaagatc agcactgtgg tgagctccaa ggaagtgaat aagtttcaga
      301 tggcttattc aaacctcctt agagctaaca tggatgggtt gaagaagaga gacaaaaagg
      361 cagcagcacc tgccgcagca gcaacagcag caacaacagc agcaacaaca gcagcaacag
      421 cagcacagta aagggcatac atttcctgct ttcaccaatt aaccactgaa ttgctatttt
      481 ttccttttgg gggatccacc ggatctagat aactgatcat aatcagccat accacatttg
      541 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
      601 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
      661 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
      721 ccaaactcat caatgtatct taacgcgtaa attgtaagcg ttaatatttt gttaaaattc
      781 gcgttaaatt tttgttaaat cagctcattt tt
//
