LOCUS       sequence-51998-e         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Van Itallie CM, Fanning AS, Bridges A, Anderson JM
  TITLE     ZO-1 stabilizes the tight junction solute barrier through coupling 
            to the perijunctional cytoskeleton.
  JOURNAL   Mol Biol Cell. 2009 Sep;20(17):3930-40. Epub 2009 Jul 15.
  PUBMED    19605556
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2009-09"; volume: "20(17)"; pages: "3930-40. Epub 
            2009 Jul 15"
COMMENT     Sequence Label: ZO-1 shRNA 3253
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        50..264
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     185..204
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(337..353)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(338..354)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(382..400)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(421..437)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(421..437)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(434..456)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    445..461
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(469..475,476..493,494..499))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    514..535
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(652..669)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ccgcgggggc ggcgctctga ctagtggatc ccccgggctg caggaattcg aacgctgacg
       61 tcatcaaccc gctccaagga atcgcgggcc cagtgtcact aggcgggaac acccagcgcg
      121 cgtgcgccct ggcaggaaga tggctgtgag ggacagggga gtggcgccct gcaatatttg
      181 catgtcgcta tgtgttctgg gaaatcacca taaacgtgaa atgtctttgg atttgggaat
      241 cttataagtt ctgtatgaga ccacagatcc ccgcgcattc ccacatatga attcaagaga
      301 ttcatatgtg ggaatgcgct ttttggaaaa gcttatcgat accgtcgacc tcgagggggg
      361 gcccggtacc agcttttgtt ccctttagtg agggttaatt tcgagcttgg cgtaatcatg
      421 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
      481 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
      541 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
      601 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
      661 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
      721 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
      781 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
      841 ccctgacgag catcacaaaa atcgacgctc aagtcagaag tggcgaancc cgacaggact
      901 ataagatacc aggcgttccc ctggaactcc tctggtctct gtcga
//
