LOCUS       sequence-51999-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Van Itallie CM, Fanning AS, Bridges A, Anderson JM
  TITLE     ZO-1 stabilizes the tight junction solute barrier through coupling 
            to the perijunctional cytoskeleton.
  JOURNAL   Mol Biol Cell. 2009 Sep;20(17):3930-40. Epub 2009 Jul 15.
  PUBMED    19605556
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2009-09"; volume: "20(17)"; pages: "3930-40. Epub 
            2009 Jul 15"
COMMENT     Sequence Label: ZO-1 shRNA 2072
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        49..263
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     184..203
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(336..352)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(337..353)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(381..399)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(420..436)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(420..436)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(433..455)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    444..460
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(468..474,475..492,493..498))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    513..534
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(651..668)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ccgcggggcg gcgctctgac tagtggatcc cccgggctgc aggaattcga acgctgacgt
       61 catcaacccg ctccaaggaa tcgcgggccc agtgtcacta ggcgggaaca cccagcgcgc
      121 gtgcgccctg gcaggaagat ggctgtgagg gacaggggag tggcgccctg caatatttgc
      181 atgtcgctat gtgttctggg aaatcaccat aaacgtgaaa tgtctttgga tttgggaatc
      241 ttataagttc tgtatgagac cacagatccc cggacaaaca tgctttatta ttcaagagat
      301 aataaagcat gtttgtcctt tttggaaaag cttatcgata ccgtcgacct cgaggggggg
      361 cccggtacca gcttttgttc cctttagtga gggttaattt cgagcttggc gtaatcatgg
      421 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
      481 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
      541 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
      601 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
      661 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
      721 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
      781 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt tt
//
