LOCUS       sequence-52003-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Van Itallie CM, Fanning AS, Bridges A, Anderson JM
  TITLE     ZO-1 stabilizes the tight junction solute barrier through coupling 
            to the perijunctional cytoskeleton.
  JOURNAL   Mol Biol Cell. 2009 Sep;20(17):3930-40. Epub 2009 Jul 15.
  PUBMED    19605556
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2009-09"; volume: "20(17)"; pages: "3930-40. Epub 
            2009 Jul 15"
COMMENT     Sequence Label: ZO-2 shRNA 1320
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        43..257
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     178..197
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(331..347)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(332..348)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(376..394)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(415..431)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(415..431)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(428..450)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    439..455
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(463..469,470..487,488..493))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    508..529
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(646..663)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggcggcgctc tgactagtgg atcccccggg ctgcaggaat tcgaacgctg acgtcatcaa
       61 cccgctccaa ggaatcgcgg gcccagtgtc actaggcggg aacacccagc gcgcgtgcgc
      121 cctggcagga agatggctgt gagggacagg ggagtggcgc cctgcaatat ttgcatgtcg
      181 ctatgtgttc tgggaaatca ccataaacgt gaaatgtctt tggatttggg aatcttataa
      241 gttctgtatg agaccacaga atccccggag tcagatcttc ataaattcaa gagatttatg
      301 aagatctgac tcctttttgg aaaagcttat cgataccgtc gacctcgagg gggggcccgg
      361 taccagcttt tgttcccttt agtgagggtt aatttcgagc ttggcgtaat catggtcata
      421 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
      481 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
      541 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
      601 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
      661 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
      721 gttatccaca gaatcagggg ataacgcang aaagaacatg tgagcaaaag gccagcaaaa
      781 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
      841 cgagcatcac aaaaatcgac gctcaagtca
//
