LOCUS       sequence-52004-e         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Van Itallie CM, Fanning AS, Bridges A, Anderson JM
  TITLE     ZO-1 stabilizes the tight junction solute barrier through coupling 
            to the perijunctional cytoskeleton.
  JOURNAL   Mol Biol Cell. 2009 Sep;20(17):3930-40. Epub 2009 Jul 15.
  PUBMED    19605556
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2009-09"; volume: "20(17)"; pages: "3930-40. Epub 
            2009 Jul 15"
COMMENT     Sequence Label: ZO-2 shRNA 2434
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        47..261
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     182..201
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(334..350)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(335..351)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(379..397)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(418..434)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(418..434)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(431..453)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    442..458
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(466..472,473..490,491..496))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    511..532
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(649..666)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcggggcggc gctctgacta gtggatcccc cgggctgcag gaattcgaac gctgacgtca
       61 tcaacccgct ccaaggaatc gcgggcccag tgtcactagg cgggaacacc cagcgcgcgt
      121 gcgccctggc aggaagatgg ctgtgaggga caggggagtg gcgccctgca atatttgcat
      181 gtcgctatgt gttctgggaa atcaccataa acgtgaaatg tctttggatt tgggaatctt
      241 ataagttctg tatgagacca cagatccccg aagaagaact taaggaaatt caagagattt
      301 ccttaagttc ttcttctttt tggaaaagct tatcgatacc gtcgacctcg agggggggcc
      361 cggtaccagc ttttgttccc tttagtgagg gttaatttcg agcttggcgt aatcatggtc
      421 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
      481 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
      541 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
      601 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
      661 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
      721 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
      781 aagc
//
