LOCUS       sequence-52006-e         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  TITLE     mouse ZO-1 shRNA
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: mZO1-2 shRNA
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     28..44
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     29..45
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(87..120)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      708..717
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(759..780)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gatactgttc agctctgttc ttttttctcg aggtcgacgg tatcgataag ctcgcttcac
       61 gagattccag caggtcgagg gacctaataa cttcgtatag catacattat acgaagttat
      121 attaagggtt ccaagcttaa gcggccgctg aaagacccca cctgtaggtt tggcaagcta
      181 gcttaagtaa cgccattttg caaggcatgg aaaatacata actgagaata gagaagttca
      241 gatcaaggtt aggaacagag agacagcaga atatgggcca aacaggatat ctgtggtaag
      301 cagttcctgc cccggctcag ggccaagaac agatggtccc cagatgcggt cccgccctca
      361 gcagtttcta gagaaccatc agatgtttcc agggtgcccc aaggacctga aatgaccctg
      421 tgccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc
      481 ccgagctcaa taaaagagcc cacaacccct cactcggcgc gccagtcctc cgatagactg
      541 cgtcgcccgg gtacccgtat tcccaataaa gcctcttgct gtttgcatcc gaatcgtgga
      601 ctcgctgatc cttgggaggg tctcctcaga ttgattgact gcccacctcg ggggtctttc
      661 atcgaattct gcagtcgacg gtaccgcggg cccgggatcc accggtcgcc accatggtga
      721 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
      781 taaacggcca caa
//
