LOCUS       sequence-52007-e         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Rodrigo G, Landrain TE, Jaramillo A
  TITLE     De novo automated design of small RNA circuits for engineering 
            synthetic riboregulation in living cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep
            4.
  PUBMED    22949707
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-09-18"; volume: "109(38)"; pages: 
            "15271-6. Epub 2012 Sep 4"
COMMENT     Sequence Label: pSTC1
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             65..76
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             102..812
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     misc_feature    819..839
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      875..906
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 cttccatacg aactttgaaa cgcatgaact ctttgataac gtcttcggag gaagccatct
       61 agtatttctc ctctttctct agtggatccc ctagggctag ccgtaaaggc gaagagctgt
      121 tcactggtgt cgtccctatt ctggtggaac tggatggtga tgtcaacggt cataagtttt
      181 ccgtgcgtgg cgagggtgaa ggtgacgcaa ctaatggtaa actgacgctg aagttcatct
      241 gtactactgg taaactgccg gtaccttggc cgactctggt aacgacgctg acttatggtg
      301 ttcagtgctt tgctcgttat ccggaccata tgaagcagca tgacttcttc aagtccgcca
      361 tgccggaagg ctatgtgcag gaacgcacga tttcctttaa ggatgacggc acgtacaaaa
      421 cgcgtgcgga agtgaaattt gaaggcgata ccctggtaaa ccgcattgag ctgaaaggca
      481 ttgactttaa agaagacggc aatatcctgg gccataagct ggaatacaat tttaacagcc
      541 acaatgttta catcaccgcc gataaacaaa aaaatggcat taaagcgaat tttaaaattc
      601 gccacaacgt ggaggatggc agcgtgcagc tggctgatca ctaccagcaa aacactccaa
      661 tcggtgatgg tcctgttctg ctgccagaca atcactatct gagcacgcaa agcgttctgt
      721 ctaaagatcc gaacgagaaa cgcgatcata tggttctgct ggagttcgta accgcagcgg
      781 gcatcacgca tggtatggat gaactgtaca aatgatgata ctagtagcgg ccgctgcagg
      841 agtcactaag ggttagttag ttagattagc agaaagtcaa aagcctccga ccggaggctt
      901 ttgact
//
