LOCUS       sequence-52011-e         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Sarbassov DD, Guertin DA, Ali SM, Sabatini DM
  TITLE     Phosphorylation and regulation of Akt/PKB by the rictor-mTOR 
            complex.
  JOURNAL   Science 2005 Feb 18;307(5712):1098-101.
  PUBMED    15718470
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science"; 
            date: "2005-02-18"; volume: "307(5712)"; pages: "1098-101"
COMMENT     Sequence Label: mTOR_1 shRNA
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    114..231
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        280..790
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     709..727
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     primer_bind     complement(812..831)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 aggacgaaac accggtcagc gtccctacct tcttctcttc ctgtcaagaa gaaggtaggg
       61 acgctgattt ttgaattctc gacctcgaga caaatggcag tattcatcca caattttaaa
      121 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
      181 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
      241 tacagggaca gcagagatcc actttggccg cggctcgagg gggttggggt tgcgcctttt
      301 ccaaggcagc cctgggtttg cgcagggacg cggctgctct gggcgtggtt ccgggaaacg
      361 cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat
      421 cttcgccgct acccttgtgg gccccccggc gacgcttcct gctccgcccc taagtcggga
      481 aggttccttg cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac gtctcactag
      541 taccctcgca gacggacagc gccagggagc aatggcagcg cgccgaccgc gatgggctgt
      601 ggccaatagc ggctgctcag cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg
      661 ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt
      721 ctgcaagcct ccggagcgca cgtcggcagt cggctccctc gttgaccgaa tcaccgacct
      781 ctctccccag ggggatccac cggagcttac catgaccgag tacaagccca cggtgcgcct
      841 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgcgatacc
      901 cgcca
//
