LOCUS       sequence-52038-e         710 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter construct containing three hypoxia response 
            elements (24-mers) from the Pgk-1 gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Emerling BM, Weinberg F, Liu JL, Mak TW, Chandel NS
  TITLE     PTEN regulates p300-dependent hypoxia-inducible factor 1 
            transcriptional activity through Forkhead transcription factor 3a 
            (FOXO3a).
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Feb 19. 105(7):2622-7.
  PUBMED    18268343
REFERENCE   2  (bases 1 to 710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2008 Feb 19. 105(7):2622-7."
COMMENT     Sequence Label: HRE-luciferase
FEATURES             Location/Qualifiers
     source          1..710
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    32..153
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(69..88)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     123..142
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        354..416
                     /label=Mini-TK promoter
                     /note="minimal herpes simplex virus (HSV) thymidine kinase 
                     promoter"
     primer_bind     complement(542..560)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 tgaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
       61 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
      121 ttgtggtttg tccaaactca tcaatgtatc ttatggtact gtaactgagc taacataacc
      181 cgggaggtac cgagctctgt cacgtcctgc acgactctng ttgtcacgtc ctgcacgact
      241 ctngttgtca cgtcctgcac gacgctagct cgagatccgg ccccgcccag cgtcttgtca
      301 ttggcgaatt cgaacacgca gatgcagtcg gggcggcgcg gtccgaggtc cacttcgcat
      361 attaaggtga cgcgtgtggc ctcgaacacc gagcgaccct gcagcgaccc gcttaacagc
      421 gtcaacagcg tgccgcagat ctaagtaagc ttggcattcc ggtactgttg gtaaaatgga
      481 agacgccnaa aacataaaga aaggcccggc gccattctat cctctagagg atgaaaccgc
      541 tggagagcaa ctgcataagg ctatgaagag atacnccctg gttcctggaa caattgcttt
      601 tacagatgca catatcgagg tgaacatcac gtacgcggaa tacttcnaaa tgtccgttcg
      661 gttggcagaa gctatgaaac gatatgggct gaatacaaat cacagaatcg
//
