LOCUS       sequence-52041-e       10230 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10230)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBACgus4x/cdc37/LRRK2 1867-2186
FEATURES             Location/Qualifiers
     source          1..10230
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(665..774)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        complement(925..1016)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     promoter        1041..1150
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        1256..1347
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             1360..1383
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             1384..1404
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2390..2407
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_recomb     2444..3801
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             complement(2460..3801)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     primer_bind     complement(3893..3910)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4064..4652)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4144..4163)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4823..5614,5615..5683))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5446..5465
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5684..5788)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5815..6270
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5902..5921)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6112..6133
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_recomb     6669..7496
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             6669..6793
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(6831..7436)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     CDS             complement(7507..9318)
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGQQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFSLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     promoter        complement(9327..9642)
                     /gene="Autographa californica p6.9"
                     /label=p6.9 promoter
                     /note="baculovirus late basic protein promoter"
ORIGIN
        1 ataaattgca tgacgattgt ctggtgggcc acctgctcca tgagtgcaca tttctcctcc
       61 acctctaggt caatgcacca aatgaccagg taattggctg tctcctcgca caccaggtgg
      121 acgttgtctg acaggtactt ttggctgtca tcccagcggc gaagcatgcc aaagtgcttg
      181 atctgtttct cgtatttttc cacgaaggtc ttgtgtttct gctccctcac ctcctctgag
      241 tcctcctccg tcttctcggg cttggtattt accatgctct tgctgaagcc gtctttgctg
      301 agcgtgtcca cgttccaggg catgctcttc tccttcttgc gcatctcctc cagcttctgc
      361 tcccagctcc gctcctcctt gcgcagctgc tgtgcctcgg cctgcaggcg ctccagctct
      421 gccttgccgc cctcggccac ctccagctcc ttcagtttcc tctggcactc ggccaccttg
      481 cgcttgcact cgcggcagcc cctgtccagt tcctccttct ccttctggaa ctgctccatg
      541 cgttccaccc gggcctgatg ccgccagcgg aagagactgg ccgtgtcgat gttggggtgc
      601 gtctcgtctt catcatcaga cacctcaatg tggtcccaca cgctgtagtc caccatggat
      661 ctgtgattgt aaataaaatg taatttacag tatagtattt taattaatat acaaatgatt
      721 tgataataat tcttatttaa ctataatata ttgtgttggg ttgaattaaa ggtcccggca
      781 tcctcaaatg cataatttca tagtccccct tgttgtaagt gatgcgtatt tctgaatctt
      841 tgtaaaatag cacacaagac tccaacgcgt ttggcgtttt attttcttgc tcgactctag
      901 tttattaggc ctctagagat ccgtatttat aggttttttt attacaaaac tgttacgaaa
      961 acagtaaaat acttatttat ttgcgagatg gttatcattt taattatctc catgatccaa
     1021 taacctagaa taaagggccc gacctttaat tcaacccaac acaatatatt atagttaaat
     1081 aagaattatt atcaaatcat ttgtatatta attaaaatac tatactgtaa attacatttt
     1141 atttacaatc acagatcccc gggactagta ataaaagatg tttattttca ttagatgtgt
     1201 gtgttggttt tttgtgtcta gtaataaaac ctagaggatc gatcccccgg atctgatcat
     1261 ggagataatt aaaatgataa ccatctcgca aataaataag tattttactg ttttcgtaac
     1321 agttttgtaa taaaaaaacc tataaatatc ggatccatgc accaccatca ccaccatcac
     1381 cacgagaacc tttatttcca aggaaacatc atgctcaaca acgatgagct cgaatttgaa
     1441 caagctccag aattcctgct cggcgacggc tctttcggct ccgtataccg tgctgcttat
     1501 gaaggcgaag aagtcgccgt aaagatcttc aacaagcaca cctcgctccg tctgctccgt
     1561 caggaactgg tcgttctgtg tcatctgcac catccgagcc tcatctccct gctggccgct
     1621 ggcatccgtc ctcgtatgct ggtgatggag ctggcatcca agggctctct cgaccgcctg
     1681 ctgcagcagg acaaggcttc tctgacccgt actctgcagc atcgtatcgc tctgcatgta
     1741 gctgacggcc tgcgttatct tcattctgca atgatcattt atcgcgacct gaaacctcat
     1801 aatgtactgc tgtttactct gtacccgaac gctgccatca tcgctaagat tgcagactat
     1861 ggtattgcgc agtactgctg ccgcatgggc attaaaacaa gcgaaggtac gccgggtttc
     1921 cgtgctccag aagtggctcg tggcaacgtt atttacaacc agcaagctga cgtttactcg
     1981 ttcggtctgc tcctgtacga tatcctgact accggcggcc gtatcgtaga aggtctgaag
     2041 ttccctaacg aattcgacga actggaaatc cagggcaagc tgccagaccc ggttaaagaa
     2101 tacggttgcg ctccgtggcc gatggtagag aagctcatca aacagtgcct taaagaaaac
     2161 cctcaggaac gtccaacctc tgctcaagtg ttcgacatcc ttaacagcgc tgaactcgtc
     2221 tgtctgactc gccgtattct cctgcctaag aatgttatcg tggaatgtat ggttgctact
     2281 caccataact ctcgcaacgc ttccatctgg ctcggctgtg gccacactga ccgtggtcag
     2341 ctcagcttcc tggacctgaa cacttgataa gcttgcggcc gcactcgagc accaccacca
     2401 ccaccactaa cctaggtagc tgagcgcatg caagctgatc cgggttatta gtacatttat
     2461 taagcgctag attctgtgcg ttgttgattt acagacaatt gttgtacgta ttttaataat
     2521 tcattaaatt tataatcttt agggtggtat gttagagcga aaatcaaatg attttcagcg
     2581 tctttatatc tgaatttaaa tattaaatcc tcaatagatt tgtaaaatag gtttcgatta
     2641 gtttcaaaca agggttgttt ttccgaaccg atggctggac tatctaatgg attttcgctc
     2701 aacgccacaa aacttgccaa atcttgtagc agcaatctag ctttgtcgat attcgtttgt
     2761 gttttgtttt gtaataaagg ttcgacgtcg ttcaaaatat tatgcgcttt tgtatttctt
     2821 tcatcactgt cgttagtgta caattgactc gacgtaaaca cgttaaatag agcttggaca
     2881 tatttaacat cgggcgtgtt agctttatta ggccgattat cgtcgtcgtc ccaaccctcg
     2941 tcgttagaag ttgcttccga agacgatttt gccatagcca cacgacgcct attaattgtg
     3001 tcggctaaca cgtccgcgat caaatttgta gttgagcttt ttggaattat ttctgattgc
     3061 gggcgttttt gggcgggttt caatctaact gtgcccgatt ttaattcaga caacacgtta
     3121 gaaagcgatg gtgcaggcgg tggtaacatt tcagacggca aatctactaa tggcggcggt
     3181 ggtggagctg atgataaatc taccatcggt ggaggcgcag gcggggctgg cggcggaggc
     3241 ggaggcggag gtggtggcgg tgatgcagac ggcggtttag gctcaaatgt ctctttaggc
     3301 aacacagtcg gcacctcaac tattgtactg gtttcgggcg ccgtttttgg tttgaccggt
     3361 ctgagacgag tgcgattttt ttcgtttcta atagcttcca acaattgttg tctgtcgtct
     3421 aaaggtgcag cgggttgagg ttccgtcggc attggtggag cgggcggcaa ttcagacatc
     3481 gatggtggtg gtggtggtgg aggcgctgga atgttaggca cgggagaagg tggtggcggc
     3541 ggtgccgccg gtataatttg ttctggttta gtttgttcgc gcacgattgt gggcaccggc
     3601 gcaggcgccg ctggctgcac aacggaaggt cgtctgcttc gaggcagcgc ttggggtggt
     3661 ggcaattcaa tattataatt ggaatacaaa tcgtaaaaat ctgctataag cattgtaatt
     3721 tcgctatcgt ttaccgtgcc gatatttaac aaccgctcaa tgtaagcaat tgtattgtaa
     3781 agagattgtc tcaagctcgg atcgatcccg cacgccgata acaagccttt tcatttttac
     3841 tacagcattg tagtggcgag acacttcgct gtcgtcgagg tttaaacgct tcctcgctca
     3901 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     3961 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     4021 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     4081 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     4141 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     4201 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     4261 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     4321 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     4381 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     4441 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     4501 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     4561 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     4621 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     4681 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     4741 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     4801 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     4861 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     4921 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     4981 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     5041 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     5101 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     5161 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     5221 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     5281 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     5341 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     5401 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     5461 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     5521 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     5581 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     5641 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     5701 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     5761 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgcgc
     5821 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     5881 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     5941 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     6001 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     6061 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     6121 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     6181 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     6241 attttaacaa aatattaacg tttacaattt cccattcgcc attcaggctg cgcaactgtt
     6301 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca ggaacggctc cgcccactat
     6361 taatgaaatt aaaaattcca attttaaaaa acgcagcaag agaaacattt gtatgaaaga
     6421 atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac aacaagatta atatgcctcc
     6481 gtgtataaaa aaaatattga acgatttgaa agaaaacaat gtaccgcgcg gcggtatgta
     6541 caggaagagg tttatactaa actgttacat tgcaaacgtg gtttcgtgtg ccaagtgtga
     6601 aaaccgatgt ttaatcaagg ctctgacgca tttctacaac cacgactcca agtgtgtggg
     6661 tgaagtcatg catcttttaa tcaaatccca agatgtgtat aaaccaccaa actgccaaaa
     6721 aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac tgcaagggtc tcaatcctat
     6781 ttgtaattat tgaataataa aacaattata aatgctaaat ttgtttttta ttaacgatac
     6841 aaaccaaacg caacaagaac atttgtagta ttatctataa ttgaaaacgc gtagttataa
     6901 tcgctgaggt aatatttaaa atcattttca aatgattcac agttaatttg cgacaatata
     6961 attttatttt cacataaact agacgccttg tcgtcttctt cttcgtattc cttctctttt
     7021 tcatttttct cttcataaaa attaacatag ttattatcgt atccatatat gtatctatcg
     7081 tatagagtaa attttttgtt gtcataaata tatatgtctt ttttaatggg gtgtatagta
     7141 ccgctgcgca tagtttttct gtaatttaca acagtgctat tttctggtag ttcttcggag
     7201 tgtgttgctt taattattaa atttatataa tcaatgaatt tgggatcgtc ggttttgtac
     7261 aatatgttgc cggcatagta cgcagcttct tctagttcaa ttacaccatt ttttagcagc
     7321 accggattaa cataactttc caaaatgttg tacgaaccgt taaacaaaaa cagttcacct
     7381 cccttttcta tactattgtc tgcgagcagt tgtttgttgt taaaaataac agccattgta
     7441 atgagacgca caaactaata tcacaaactg gaaatgtcta tcaatatata gttgctgatc
     7501 agatcctcat tgtttgcctc cctgctgcgg tttttcaccg aagttcatgc cagtccagcg
     7561 tttttgcagc agaaaagccg ccgacttcgg tttgcggtcg cgagtgaaga tccctttctt
     7621 gttaccgcca acgcgcaata tgccttgcga ggtcgcaaaa tcggcgaaat tccatacctg
     7681 ttcaccgacg acggcgctga cgcgatcaaa gacgcggtga tacatatcca gccatgcaca
     7741 ctgatactct tcactccaca tgtcggtgta cattgagtgc agcccggcta acgtatccac
     7801 gccgtattcg gtgatgataa tcggctgatg cagtttctcc tgccaggcca gaagttcttt
     7861 ttccagtacc ttctctgccg tttccaaatc gccgctttgg acataccatc cgtaataacg
     7921 gttcaggcac agcacatcaa agagatcgct gatggtatcg gtgtgagcgt cgcagaacat
     7981 tacattgacg caggtgatcg gacgcgtcgg gtcgagttta cgcgttgctt ccgccagtgg
     8041 cgcgaaatat tcccgtgcac cttgcggacg ggtatccggt tcgttggcaa tactccacat
     8101 caccacgctt gggtggtttt tgtcacgcgc tatcagctct ttaatcgcct gtaagtgcgc
     8161 ttgctgagtt tccccgttga ctgcctcttc gctgtacagt tctttcggct tgttgcccgc
     8221 ttcgaaacca atgcctaaag agagcgaaaa gccgacagca gcagtttcat caatcaccac
     8281 gatgccatgt tcatctgccc agtcgagcat ctcttcagcg taagggtaat gcgaggtacg
     8341 gtaggagttg gccccaatcc agtccattaa tgcgtggtcg tgcaccatca gcacgttatc
     8401 gaatcctttg ccacgtaagt ccgcatcttc atgacgacca aagccagtaa agtagaacgg
     8461 tttgtggtta atcaggaact gttggccctt cactgccact gaccggatgc cgacgcgaag
     8521 cgggtagata tcacactctg tctggctttt ggctgtgacg cacagttcat agagataacc
     8581 ttcacccggt tgccagaggt gcggattcac cacttgcaaa gtcccgctag tgccttgtcc
     8641 agttgcaacc acctgttgat ccgcatcacg cagttcaacg ctgacatcac cattggccac
     8701 cacctgccag tcaacagacg cgtggttaca gtcttgcgcg acatgcgtca ccacggtgat
     8761 atcgtccacc caggtgttcg gcgtggtgta gagcattacg ctgcgatgga ttccggcata
     8821 gttaaagaaa tcatggaagt aagactgctt tttcttgccg ttttcgtcgg taatcaccat
     8881 tcccggcggg atagtctgcc agttcagttc gttgttcaca caaacggtga tacgtacact
     8941 tttcccggca ataacatacg gcgtgacatc ggcttcaaat ggcgtatagc cgccctgatg
     9001 ctccatcact tcctgattat tgacccacac tttgccgtaa tgagtgaccg catcgaaacg
     9061 cagcacgata cgctggcctg cccaaccttt cggtataaag acttcgcgct gataccagac
     9121 gttgcccgca taattacgaa tatctgcatc ggcgaactga tcgttaaaac tgcctggcac
     9181 agcaattgcc cggctttctt gtaacgcgct ttcccaccaa cgctgatcaa ttccacagtt
     9241 ttcgcgatcc agactgaatg cccacaggcc gtcgagtttt ttgatttcac gggttggggt
     9301 ttctacagga cggaccatat ttataggtgt aatttatgta gctgtaattt ttaccttatt
     9361 aatatttttt acgctttgca ttcgacgact gaactcccaa atatatgttt aactcgtctt
     9421 ggtcgtttga atttttgttg ctgtgtttcc taatattttc catcacctta aatatgttat
     9481 tgtaatcctc aatgttgaac ttgcaattgg acacggcata gttttccata gtcgtgtaaa
     9541 acatggtatt ggctgcattg taatacatcc gactgagcgg gtacggatct atgtgtttga
     9601 gcagcctgtt caaaaactct gcatcgtcgc aaaacggaat ttcgtcgaca gatcgtaacc
     9661 acacaaaaaa ccaacacaca gttctaatga aaataaagaa cttttattat cctcaggtct
     9721 agctggaatt cttatcaggt ttcaggctcg ggcacactga catccttctc atcgcccgtc
     9781 ttgggaacag cttccagtaa tgggtcccca ggacctgcct cctctccctc cttggcctcg
     9841 ctggccttag agttggggac ccagaggcca gagtcaatgc agcgctgcat gtggtacttt
     9901 gcgtcggtgg ggtccatctt gctgatggcg tcctgcagca tctgcacgtc cttcacatcg
     9961 aagcacttct ggagttcctc agggagggac tcgtagacct cgacggggtc caggccgccg
    10021 gggccgagcc gcttcttgcg ctcctcctcc tcgtactcct tcatggcctt ctcgatgcgc
    10081 agcttggcac ggccccgcac acgctccttg aaggcttcca gctcgtcgtt gaagccctcc
    10141 atgtactggc gatcggctgt cttaatctta gtgaagaact gccggaagca ggcccggggg
    10201 tccaccttta ggctcttggc cagctccagg
//
