LOCUS       sequence-52047-e       10200 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10200)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10200)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBACgus4x/cdc37/LRRK2 1867-2176
FEATURES             Location/Qualifiers
     source          1..10200
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(665..774)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        complement(925..1016)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     promoter        1041..1150
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        1256..1347
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             1360..1383
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             1384..1404
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2360..2377
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_recomb     2414..3771
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             complement(2430..3771)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     primer_bind     complement(3863..3880)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4034..4622)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4114..4133)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4793..5584,5585..5653))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5416..5435
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5654..5758)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5785..6240
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5872..5891)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6082..6103
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_recomb     6639..7466
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             6639..6763
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(6801..7406)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     CDS             complement(7477..9288)
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGQQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFSLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     promoter        complement(9297..9612)
                     /gene="Autographa californica p6.9"
                     /label=p6.9 promoter
                     /note="baculovirus late basic protein promoter"
ORIGIN
        1 ataaattgca tgacgattgt ctggtgggcc acctgctcca tgagtgcaca tttctcctcc
       61 acctctaggt caatgcacca aatgaccagg taattggctg tctcctcgca caccaggtgg
      121 acgttgtctg acaggtactt ttggctgtca tcccagcggc gaagcatgcc aaagtgcttg
      181 atctgtttct cgtatttttc cacgaaggtc ttgtgtttct gctccctcac ctcctctgag
      241 tcctcctccg tcttctcggg cttggtattt accatgctct tgctgaagcc gtctttgctg
      301 agcgtgtcca cgttccaggg catgctcttc tccttcttgc gcatctcctc cagcttctgc
      361 tcccagctcc gctcctcctt gcgcagctgc tgtgcctcgg cctgcaggcg ctccagctct
      421 gccttgccgc cctcggccac ctccagctcc ttcagtttcc tctggcactc ggccaccttg
      481 cgcttgcact cgcggcagcc cctgtccagt tcctccttct ccttctggaa ctgctccatg
      541 cgttccaccc gggcctgatg ccgccagcgg aagagactgg ccgtgtcgat gttggggtgc
      601 gtctcgtctt catcatcaga cacctcaatg tggtcccaca cgctgtagtc caccatggat
      661 ctgtgattgt aaataaaatg taatttacag tatagtattt taattaatat acaaatgatt
      721 tgataataat tcttatttaa ctataatata ttgtgttggg ttgaattaaa ggtcccggca
      781 tcctcaaatg cataatttca tagtccccct tgttgtaagt gatgcgtatt tctgaatctt
      841 tgtaaaatag cacacaagac tccaacgcgt ttggcgtttt attttcttgc tcgactctag
      901 tttattaggc ctctagagat ccgtatttat aggttttttt attacaaaac tgttacgaaa
      961 acagtaaaat acttatttat ttgcgagatg gttatcattt taattatctc catgatccaa
     1021 taacctagaa taaagggccc gacctttaat tcaacccaac acaatatatt atagttaaat
     1081 aagaattatt atcaaatcat ttgtatatta attaaaatac tatactgtaa attacatttt
     1141 atttacaatc acagatcccc gggactagta ataaaagatg tttattttca ttagatgtgt
     1201 gtgttggttt tttgtgtcta gtaataaaac ctagaggatc gatcccccgg atctgatcat
     1261 ggagataatt aaaatgataa ccatctcgca aataaataag tattttactg ttttcgtaac
     1321 agttttgtaa taaaaaaacc tataaatatc ggatccatgc accaccatca ccaccatcac
     1381 cacgagaacc tttatttcca aggaaacatc atgctcaaca acgatgagct cgaatttgaa
     1441 caagctccag aattcctgct cggcgacggc tctttcggct ccgtataccg tgctgcttat
     1501 gaaggcgaag aagtcgccgt aaagatcttc aacaagcaca cctcgctccg tctgctccgt
     1561 caggaactgg tcgttctgtg tcatctgcac catccgagcc tcatctccct gctggccgct
     1621 ggcatccgtc ctcgtatgct ggtgatggag ctggcatcca agggctctct cgaccgcctg
     1681 ctgcagcagg acaaggcttc tctgacccgt actctgcagc atcgtatcgc tctgcatgta
     1741 gctgacggcc tgcgttatct tcattctgca atgatcattt atcgcgacct gaaacctcat
     1801 aatgtactgc tgtttactct gtacccgaac gctgccatca tcgctaagat tgcagactat
     1861 ggtattgcgc agtactgctg ccgcatgggc attaaaacaa gcgaaggtac gccgggtttc
     1921 cgtgctccag aagtggctcg tggcaacgtt atttacaacc agcaagctga cgtttactcg
     1981 ttcggtctgc tcctgtacga tatcctgact accggcggcc gtatcgtaga aggtctgaag
     2041 ttccctaacg aattcgacga actggaaatc cagggcaagc tgccagaccc ggttaaagaa
     2101 tacggttgcg ctccgtggcc gatggtagag aagctcatca aacagtgcct taaagaaaac
     2161 cctcaggaac gtccaacctc tgctcaagtg ttcgacatcc ttaacagcgc tgaactcgtc
     2221 tgtctgactc gccgtattct cctgcctaag aatgttatcg tggaatgtat ggttgctact
     2281 caccataact ctcgcaacgc ttccatctgg ctcggctgtg gccacactga ccgttgataa
     2341 gcttgcggcc gcactcgagc accaccacca ccaccactaa cctaggtagc tgagcgcatg
     2401 caagctgatc cgggttatta gtacatttat taagcgctag attctgtgcg ttgttgattt
     2461 acagacaatt gttgtacgta ttttaataat tcattaaatt tataatcttt agggtggtat
     2521 gttagagcga aaatcaaatg attttcagcg tctttatatc tgaatttaaa tattaaatcc
     2581 tcaatagatt tgtaaaatag gtttcgatta gtttcaaaca agggttgttt ttccgaaccg
     2641 atggctggac tatctaatgg attttcgctc aacgccacaa aacttgccaa atcttgtagc
     2701 agcaatctag ctttgtcgat attcgtttgt gttttgtttt gtaataaagg ttcgacgtcg
     2761 ttcaaaatat tatgcgcttt tgtatttctt tcatcactgt cgttagtgta caattgactc
     2821 gacgtaaaca cgttaaatag agcttggaca tatttaacat cgggcgtgtt agctttatta
     2881 ggccgattat cgtcgtcgtc ccaaccctcg tcgttagaag ttgcttccga agacgatttt
     2941 gccatagcca cacgacgcct attaattgtg tcggctaaca cgtccgcgat caaatttgta
     3001 gttgagcttt ttggaattat ttctgattgc gggcgttttt gggcgggttt caatctaact
     3061 gtgcccgatt ttaattcaga caacacgtta gaaagcgatg gtgcaggcgg tggtaacatt
     3121 tcagacggca aatctactaa tggcggcggt ggtggagctg atgataaatc taccatcggt
     3181 ggaggcgcag gcggggctgg cggcggaggc ggaggcggag gtggtggcgg tgatgcagac
     3241 ggcggtttag gctcaaatgt ctctttaggc aacacagtcg gcacctcaac tattgtactg
     3301 gtttcgggcg ccgtttttgg tttgaccggt ctgagacgag tgcgattttt ttcgtttcta
     3361 atagcttcca acaattgttg tctgtcgtct aaaggtgcag cgggttgagg ttccgtcggc
     3421 attggtggag cgggcggcaa ttcagacatc gatggtggtg gtggtggtgg aggcgctgga
     3481 atgttaggca cgggagaagg tggtggcggc ggtgccgccg gtataatttg ttctggttta
     3541 gtttgttcgc gcacgattgt gggcaccggc gcaggcgccg ctggctgcac aacggaaggt
     3601 cgtctgcttc gaggcagcgc ttggggtggt ggcaattcaa tattataatt ggaatacaaa
     3661 tcgtaaaaat ctgctataag cattgtaatt tcgctatcgt ttaccgtgcc gatatttaac
     3721 aaccgctcaa tgtaagcaat tgtattgtaa agagattgtc tcaagctcgg atcgatcccg
     3781 cacgccgata acaagccttt tcatttttac tacagcattg tagtggcgag acacttcgct
     3841 gtcgtcgagg tttaaacgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     3901 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     3961 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     4021 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     4081 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     4141 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     4201 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     4261 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     4321 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     4381 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     4441 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     4501 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     4561 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     4621 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     4681 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     4741 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     4801 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     4861 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     4921 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     4981 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     5041 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     5101 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     5161 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     5221 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     5281 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     5341 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     5401 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     5461 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     5521 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     5581 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     5641 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     5701 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     5761 catttccccg aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     5821 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     5881 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     5941 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     6001 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     6061 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     6121 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     6181 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt
     6241 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     6301 tattacgcca ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa
     6361 acgcagcaag agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga
     6421 catgctgaac aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa
     6481 agaaaacaat gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat
     6541 tgcaaacgtg gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca
     6601 tttctacaac cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca
     6661 agatgtgtat aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt
     6721 tgctggcaac tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata
     6781 aatgctaaat ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta
     6841 ttatctataa ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca
     6901 aatgattcac agttaatttg cgacaatata attttatttt cacataaact agacgccttg
     6961 tcgtcttctt cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag
     7021 ttattatcgt atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata
     7081 tatatgtctt ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca
     7141 acagtgctat tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa
     7201 tcaatgaatt tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct
     7261 tctagttcaa ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg
     7321 tacgaaccgt taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt
     7381 tgtttgttgt taaaaataac agccattgta atgagacgca caaactaata tcacaaactg
     7441 gaaatgtcta tcaatatata gttgctgatc agatcctcat tgtttgcctc cctgctgcgg
     7501 tttttcaccg aagttcatgc cagtccagcg tttttgcagc agaaaagccg ccgacttcgg
     7561 tttgcggtcg cgagtgaaga tccctttctt gttaccgcca acgcgcaata tgccttgcga
     7621 ggtcgcaaaa tcggcgaaat tccatacctg ttcaccgacg acggcgctga cgcgatcaaa
     7681 gacgcggtga tacatatcca gccatgcaca ctgatactct tcactccaca tgtcggtgta
     7741 cattgagtgc agcccggcta acgtatccac gccgtattcg gtgatgataa tcggctgatg
     7801 cagtttctcc tgccaggcca gaagttcttt ttccagtacc ttctctgccg tttccaaatc
     7861 gccgctttgg acataccatc cgtaataacg gttcaggcac agcacatcaa agagatcgct
     7921 gatggtatcg gtgtgagcgt cgcagaacat tacattgacg caggtgatcg gacgcgtcgg
     7981 gtcgagttta cgcgttgctt ccgccagtgg cgcgaaatat tcccgtgcac cttgcggacg
     8041 ggtatccggt tcgttggcaa tactccacat caccacgctt gggtggtttt tgtcacgcgc
     8101 tatcagctct ttaatcgcct gtaagtgcgc ttgctgagtt tccccgttga ctgcctcttc
     8161 gctgtacagt tctttcggct tgttgcccgc ttcgaaacca atgcctaaag agagcgaaaa
     8221 gccgacagca gcagtttcat caatcaccac gatgccatgt tcatctgccc agtcgagcat
     8281 ctcttcagcg taagggtaat gcgaggtacg gtaggagttg gccccaatcc agtccattaa
     8341 tgcgtggtcg tgcaccatca gcacgttatc gaatcctttg ccacgtaagt ccgcatcttc
     8401 atgacgacca aagccagtaa agtagaacgg tttgtggtta atcaggaact gttggccctt
     8461 cactgccact gaccggatgc cgacgcgaag cgggtagata tcacactctg tctggctttt
     8521 ggctgtgacg cacagttcat agagataacc ttcacccggt tgccagaggt gcggattcac
     8581 cacttgcaaa gtcccgctag tgccttgtcc agttgcaacc acctgttgat ccgcatcacg
     8641 cagttcaacg ctgacatcac cattggccac cacctgccag tcaacagacg cgtggttaca
     8701 gtcttgcgcg acatgcgtca ccacggtgat atcgtccacc caggtgttcg gcgtggtgta
     8761 gagcattacg ctgcgatgga ttccggcata gttaaagaaa tcatggaagt aagactgctt
     8821 tttcttgccg ttttcgtcgg taatcaccat tcccggcggg atagtctgcc agttcagttc
     8881 gttgttcaca caaacggtga tacgtacact tttcccggca ataacatacg gcgtgacatc
     8941 ggcttcaaat ggcgtatagc cgccctgatg ctccatcact tcctgattat tgacccacac
     9001 tttgccgtaa tgagtgaccg catcgaaacg cagcacgata cgctggcctg cccaaccttt
     9061 cggtataaag acttcgcgct gataccagac gttgcccgca taattacgaa tatctgcatc
     9121 ggcgaactga tcgttaaaac tgcctggcac agcaattgcc cggctttctt gtaacgcgct
     9181 ttcccaccaa cgctgatcaa ttccacagtt ttcgcgatcc agactgaatg cccacaggcc
     9241 gtcgagtttt ttgatttcac gggttggggt ttctacagga cggaccatat ttataggtgt
     9301 aatttatgta gctgtaattt ttaccttatt aatatttttt acgctttgca ttcgacgact
     9361 gaactcccaa atatatgttt aactcgtctt ggtcgtttga atttttgttg ctgtgtttcc
     9421 taatattttc catcacctta aatatgttat tgtaatcctc aatgttgaac ttgcaattgg
     9481 acacggcata gttttccata gtcgtgtaaa acatggtatt ggctgcattg taatacatcc
     9541 gactgagcgg gtacggatct atgtgtttga gcagcctgtt caaaaactct gcatcgtcgc
     9601 aaaacggaat ttcgtcgaca gatcgtaacc acacaaaaaa ccaacacaca gttctaatga
     9661 aaataaagaa cttttattat cctcaggtct agctggaatt cttatcaggt ttcaggctcg
     9721 ggcacactga catccttctc atcgcccgtc ttgggaacag cttccagtaa tgggtcccca
     9781 ggacctgcct cctctccctc cttggcctcg ctggccttag agttggggac ccagaggcca
     9841 gagtcaatgc agcgctgcat gtggtacttt gcgtcggtgg ggtccatctt gctgatggcg
     9901 tcctgcagca tctgcacgtc cttcacatcg aagcacttct ggagttcctc agggagggac
     9961 tcgtagacct cgacggggtc caggccgccg gggccgagcc gcttcttgcg ctcctcctcc
    10021 tcgtactcct tcatggcctt ctcgatgcgc agcttggcac ggccccgcac acgctccttg
    10081 aaggcttcca gctcgtcgtt gaagccctcc atgtactggc gatcggctgt cttaatctta
    10141 gtgaagaact gccggaagca ggcccggggg tccaccttta ggctcttggc cagctccagg
//
