LOCUS       sequence-52073-e       11787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11787)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBACgus4x/cdc37/RocCOR LRRK2 1867-2141
FEATURES             Location/Qualifiers
     source          1..11787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(665..774)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        complement(925..1016)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     promoter        1041..1150
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        1256..1347
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             1366..1389
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             1390..1410
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             3947..3964
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_recomb     4001..5358
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             complement(4017..5358)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     primer_bind     complement(5450..5467)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5621..6209)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5701..5720)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6380..7171,7172..7240))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7003..7022
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7241..7345)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7372..7827
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7459..7478)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7669..7690
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_recomb     8226..9053
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             8226..8350
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(8388..8993)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     CDS             complement(9064..10875)
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGQQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFSLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     promoter        complement(10884..11199)
                     /gene="Autographa californica p6.9"
                     /label=p6.9 promoter
                     /note="baculovirus late basic protein promoter"
ORIGIN
        1 ataaattgca tgacgattgt ctggtgggcc acctgctcca tgagtgcaca tttctcctcc
       61 acctctaggt caatgcacca aatgaccagg taattggctg tctcctcgca caccaggtgg
      121 acgttgtctg acaggtactt ttggctgtca tcccagcggc gaagcatgcc aaagtgcttg
      181 atctgtttct cgtatttttc cacgaaggtc ttgtgtttct gctccctcac ctcctctgag
      241 tcctcctccg tcttctcggg cttggtattt accatgctct tgctgaagcc gtctttgctg
      301 agcgtgtcca cgttccaggg catgctcttc tccttcttgc gcatctcctc cagcttctgc
      361 tcccagctcc gctcctcctt gcgcagctgc tgtgcctcgg cctgcaggcg ctccagctct
      421 gccttgccgc cctcggccac ctccagctcc ttcagtttcc tctggcactc ggccaccttg
      481 cgcttgcact cgcggcagcc cctgtccagt tcctccttct ccttctggaa ctgctccatg
      541 cgttccaccc gggcctgatg ccgccagcgg aagagactgg ccgtgtcgat gttggggtgc
      601 gtctcgtctt catcatcaga cacctcaatg tggtcccaca cgctgtagtc caccatggat
      661 ctgtgattgt aaataaaatg taatttacag tatagtattt taattaatat acaaatgatt
      721 tgataataat tcttatttaa ctataatata ttgtgttggg ttgaattaaa ggtcccggca
      781 tcctcaaatg cataatttca tagtccccct tgttgtaagt gatgcgtatt tctgaatctt
      841 tgtaaaatag cacacaagac tccaacgcgt ttggcgtttt attttcttgc tcgactctag
      901 tttattaggc ctctagagat ccgtatttat aggttttttt attacaaaac tgttacgaaa
      961 acagtaaaat acttatttat ttgcgagatg gttatcattt taattatctc catgatccaa
     1021 taacctagaa taaagggccc gacctttaat tcaacccaac acaatatatt atagttaaat
     1081 aagaattatt atcaaatcat ttgtatatta attaaaatac tatactgtaa attacatttt
     1141 atttacaatc acagatcccc gggactagta ataaaagatg tttattttca ttagatgtgt
     1201 gtgttggttt tttgtgtcta gtaataaaac ctagaggatc gatcccccgg atctgatcat
     1261 ggagataatt aaaatgataa ccatctcgca aataaataag tattttactg ttttcgtaac
     1321 agttttgtaa taaaaaaacc tataaatatc ggatccggat ccatgcacca ccatcaccac
     1381 catcaccacg agaaccttta tttccaagga aattttgact ttaaacatat tgggtgtaaa
     1441 gcaaaagaca ttattaggtt cttgcagcaa cgccttaaga aggcagtgcc gtacaataga
     1501 atgaaactta tgatcgttgg taatacgggt tctggtaaaa cgactctatt acaacagttg
     1561 atgaaaacga agaagagcga tctaggaatg caaagcgcta ccgtcggcat agacgtcaaa
     1621 gattggccta tacaaatacg ggacaaacgc aaacgtgacc tcgtgttgaa cgtttgggac
     1681 tttgctggcc gggaagaatt ttactctaca catccgcatt ttatgactca acgcgctttg
     1741 taccttgcag tgtatgattt gtccaaggga caggctgagg tcgacgcaat gaagccctgg
     1801 ttgtttaata ttaaagcgcg cgcatcatcg tctccggtga ttcttgtcgg aacccatctc
     1861 gatgtgagtg acgagaaaca acgcaaagcg tgcatgagta aaatcacaaa agaattactg
     1921 aataaacgag gttttccggc gatacgcgat tatcactttg tgaatgcaac tgaagaatcc
     1981 gacgctttgg cgaaattgcg caaaaccatt ataaatgaga gcctaaactt taaaatacgc
     2041 gaccaattag tggtgggtca acttattccg gattgctacg tagagttgga aaagataatt
     2101 ctctctgaac gcaagaacgt gcccattgaa tttccggtga tcgaccgtaa acgtcttttg
     2161 caattggtcc gtgaaaatca attgcaattg gacgagaacg aattgcctca cgcggtgcac
     2221 ttcttaaacg agagcggcgt cttgcttcat tttcaggacc cggcgttgca actgagtgac
     2281 ctatacttcg tggaaccgaa atggctatgt aagatcatgg cgcaaattct gactgttaaa
     2341 gttgaaggtt gtcctaaaca tcccaaaggc ataatttcga ggcgagacgt ggagaagttc
     2401 ttgagtaaga aacgtaaatt tccgaagaat tacatgagcc aatactttaa attgttggag
     2461 aaatttcaga ttgcgttgcc gattggagaa gaatacttac tggtcccgag ctcgctgagc
     2521 gaccatcgcc cggttattga actaccgcat tgcgagaaca gcgagataat tatacgcctg
     2581 tacgagatgc cctactttcc catgggcttc tggagcagat tgattaacag attgttggag
     2641 atcagcccct acatgctgtc aggtcgagag cgcgcactac gccccaatcg tatgtattgg
     2701 cggcagggca tatatttgaa ttggtcgccg gaagcatact gtctagtagg ctcagaagtc
     2761 ttagataatc acccggagtc ttttcttaaa attaccgtgc cgagctgccg taaagggtgt
     2821 atcttgttag gccaagtggt cgaccacatt gatagcttga tggaagaatg gtttccaggc
     2881 ctccttgaaa tcgacatttg cggcgagggc gagacactct tgaagaagtg ggcgttgtat
     2941 tcttttaacg atggcgagga gcaccagaag atactgctag atgacctgat gaagaaagcg
     3001 gaagaaggcg acctgttggt gaaccctgac cagccgagat tgacgatacc catttcacaa
     3061 atagcacctg acctaatttt ggccgatttg cctcgcaata ttatgctaaa caacgacgag
     3121 ttagagtttg agcaagctcc cgaatttcta ctaggcgacg gttcttttgg ctcggtatac
     3181 cgagcggctt atgaaggcga agaagtggct gttaagatct tcaacaagca cacctcgctc
     3241 cgtctgctcc gtcaggaact ggtcgttctg tgtcatctgc accatccgag cctcatctcc
     3301 ctgctggccg ctggcatccg tcctcgtatg ctggtgatgg agctggcatc caagggctct
     3361 ctcgaccgcc tgctgcagca ggacaaggct tctctgaccc gtactctgca gcatcgtatc
     3421 gctctgcatg tagctgacgg cctgcgttat cttcattctg caatgatcat ttatcgcgac
     3481 ctgaaacctc ataatgtact gctgtttact ctgtacccga acgctgccat catcgctaag
     3541 attgcagact atggtattgc gcagtactgc tgccgcatgg gcattaaaac aagcgaaggt
     3601 acgccgggtt tccgtgctcc agaagtggct cgtggcaacg ttatttacaa ccagcaagct
     3661 gacgtttact cgttcggtct gctcctgtac gatatcctga ctaccggcgg ccgtatcgta
     3721 gaaggtctga agttccctaa cgaattcgac gaactggaaa tccagggcaa gctgccagac
     3781 ccggttaaag aatacggttg cgctccgtgg ccgatggtag agaagctcat caaacagtgc
     3841 cttaaagaaa accctcagga acgtccaacc tctgctcaag tgttcgacat ccttaacagc
     3901 gctgaactcg tctgtctgac ttgataagct tgcggccgca ctcgagcacc accaccacca
     3961 ccactaacct aggtagctga gcgcatgcaa gctgatccgg gttattagta catttattaa
     4021 gcgctagatt ctgtgcgttg ttgatttaca gacaattgtt gtacgtattt taataattca
     4081 ttaaatttat aatctttagg gtggtatgtt agagcgaaaa tcaaatgatt ttcagcgtct
     4141 ttatatctga atttaaatat taaatcctca atagatttgt aaaataggtt tcgattagtt
     4201 tcaaacaagg gttgtttttc cgaaccgatg gctggactat ctaatggatt ttcgctcaac
     4261 gccacaaaac ttgccaaatc ttgtagcagc aatctagctt tgtcgatatt cgtttgtgtt
     4321 ttgttttgta ataaaggttc gacgtcgttc aaaatattat gcgcttttgt atttctttca
     4381 tcactgtcgt tagtgtacaa ttgactcgac gtaaacacgt taaatagagc ttggacatat
     4441 ttaacatcgg gcgtgttagc tttattaggc cgattatcgt cgtcgtccca accctcgtcg
     4501 ttagaagttg cttccgaaga cgattttgcc atagccacac gacgcctatt aattgtgtcg
     4561 gctaacacgt ccgcgatcaa atttgtagtt gagctttttg gaattatttc tgattgcggg
     4621 cgtttttggg cgggtttcaa tctaactgtg cccgatttta attcagacaa cacgttagaa
     4681 agcgatggtg caggcggtgg taacatttca gacggcaaat ctactaatgg cggcggtggt
     4741 ggagctgatg ataaatctac catcggtgga ggcgcaggcg gggctggcgg cggaggcgga
     4801 ggcggaggtg gtggcggtga tgcagacggc ggtttaggct caaatgtctc tttaggcaac
     4861 acagtcggca cctcaactat tgtactggtt tcgggcgccg tttttggttt gaccggtctg
     4921 agacgagtgc gatttttttc gtttctaata gcttccaaca attgttgtct gtcgtctaaa
     4981 ggtgcagcgg gttgaggttc cgtcggcatt ggtggagcgg gcggcaattc agacatcgat
     5041 ggtggtggtg gtggtggagg cgctggaatg ttaggcacgg gagaaggtgg tggcggcggt
     5101 gccgccggta taatttgttc tggtttagtt tgttcgcgca cgattgtggg caccggcgca
     5161 ggcgccgctg gctgcacaac ggaaggtcgt ctgcttcgag gcagcgcttg gggtggtggc
     5221 aattcaatat tataattgga atacaaatcg taaaaatctg ctataagcat tgtaatttcg
     5281 ctatcgttta ccgtgccgat atttaacaac cgctcaatgt aagcaattgt attgtaaaga
     5341 gattgtctca agctcggatc gatcccgcac gccgataaca agccttttca tttttactac
     5401 agcattgtag tggcgagaca cttcgctgtc gtcgaggttt aaacgcttcc tcgctcactg
     5461 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     5521 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     5581 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     5641 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     5701 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     5761 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     5821 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     5881 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     5941 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     6001 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     6061 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     6121 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     6181 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     6241 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     6301 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     6361 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     6421 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     6481 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     6541 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     6601 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     6661 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     6721 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     6781 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     6841 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     6901 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     6961 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     7021 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     7081 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     7141 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     7201 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     7261 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     7321 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgcgccct
     7381 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     7441 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     7501 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     7561 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     7621 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     7681 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     7741 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     7801 ttaacaaaat attaacgttt acaatttccc attcgccatt caggctgcgc aactgttggg
     7861 aagggcgatc ggtgcgggcc tcttcgctat tacgccagga acggctccgc ccactattaa
     7921 tgaaattaaa aattccaatt ttaaaaaacg cagcaagaga aacatttgta tgaaagaatg
     7981 cgtagaagga aagaaaaatg tcgtcgacat gctgaacaac aagattaata tgcctccgtg
     8041 tataaaaaaa atattgaacg atttgaaaga aaacaatgta ccgcgcggcg gtatgtacag
     8101 gaagaggttt atactaaact gttacattgc aaacgtggtt tcgtgtgcca agtgtgaaaa
     8161 ccgatgttta atcaaggctc tgacgcattt ctacaaccac gactccaagt gtgtgggtga
     8221 agtcatgcat cttttaatca aatcccaaga tgtgtataaa ccaccaaact gccaaaaaat
     8281 gaaaactgtc gacaagctct gtccgtttgc tggcaactgc aagggtctca atcctatttg
     8341 taattattga ataataaaac aattataaat gctaaatttg ttttttatta acgatacaaa
     8401 ccaaacgcaa caagaacatt tgtagtatta tctataattg aaaacgcgta gttataatcg
     8461 ctgaggtaat atttaaaatc attttcaaat gattcacagt taatttgcga caatataatt
     8521 ttattttcac ataaactaga cgccttgtcg tcttcttctt cgtattcctt ctctttttca
     8581 tttttctctt cataaaaatt aacatagtta ttatcgtatc catatatgta tctatcgtat
     8641 agagtaaatt ttttgttgtc ataaatatat atgtcttttt taatggggtg tatagtaccg
     8701 ctgcgcatag tttttctgta atttacaaca gtgctatttt ctggtagttc ttcggagtgt
     8761 gttgctttaa ttattaaatt tatataatca atgaatttgg gatcgtcggt tttgtacaat
     8821 atgttgccgg catagtacgc agcttcttct agttcaatta caccattttt tagcagcacc
     8881 ggattaacat aactttccaa aatgttgtac gaaccgttaa acaaaaacag ttcacctccc
     8941 ttttctatac tattgtctgc gagcagttgt ttgttgttaa aaataacagc cattgtaatg
     9001 agacgcacaa actaatatca caaactggaa atgtctatca atatatagtt gctgatcaga
     9061 tcctcattgt ttgcctccct gctgcggttt ttcaccgaag ttcatgccag tccagcgttt
     9121 ttgcagcaga aaagccgccg acttcggttt gcggtcgcga gtgaagatcc ctttcttgtt
     9181 accgccaacg cgcaatatgc cttgcgaggt cgcaaaatcg gcgaaattcc atacctgttc
     9241 accgacgacg gcgctgacgc gatcaaagac gcggtgatac atatccagcc atgcacactg
     9301 atactcttca ctccacatgt cggtgtacat tgagtgcagc ccggctaacg tatccacgcc
     9361 gtattcggtg atgataatcg gctgatgcag tttctcctgc caggccagaa gttctttttc
     9421 cagtaccttc tctgccgttt ccaaatcgcc gctttggaca taccatccgt aataacggtt
     9481 caggcacagc acatcaaaga gatcgctgat ggtatcggtg tgagcgtcgc agaacattac
     9541 attgacgcag gtgatcggac gcgtcgggtc gagtttacgc gttgcttccg ccagtggcgc
     9601 gaaatattcc cgtgcacctt gcggacgggt atccggttcg ttggcaatac tccacatcac
     9661 cacgcttggg tggtttttgt cacgcgctat cagctcttta atcgcctgta agtgcgcttg
     9721 ctgagtttcc ccgttgactg cctcttcgct gtacagttct ttcggcttgt tgcccgcttc
     9781 gaaaccaatg cctaaagaga gcgaaaagcc gacagcagca gtttcatcaa tcaccacgat
     9841 gccatgttca tctgcccagt cgagcatctc ttcagcgtaa gggtaatgcg aggtacggta
     9901 ggagttggcc ccaatccagt ccattaatgc gtggtcgtgc accatcagca cgttatcgaa
     9961 tcctttgcca cgtaagtccg catcttcatg acgaccaaag ccagtaaagt agaacggttt
    10021 gtggttaatc aggaactgtt ggcccttcac tgccactgac cggatgccga cgcgaagcgg
    10081 gtagatatca cactctgtct ggcttttggc tgtgacgcac agttcataga gataaccttc
    10141 acccggttgc cagaggtgcg gattcaccac ttgcaaagtc ccgctagtgc cttgtccagt
    10201 tgcaaccacc tgttgatccg catcacgcag ttcaacgctg acatcaccat tggccaccac
    10261 ctgccagtca acagacgcgt ggttacagtc ttgcgcgaca tgcgtcacca cggtgatatc
    10321 gtccacccag gtgttcggcg tggtgtagag cattacgctg cgatggattc cggcatagtt
    10381 aaagaaatca tggaagtaag actgcttttt cttgccgttt tcgtcggtaa tcaccattcc
    10441 cggcgggata gtctgccagt tcagttcgtt gttcacacaa acggtgatac gtacactttt
    10501 cccggcaata acatacggcg tgacatcggc ttcaaatggc gtatagccgc cctgatgctc
    10561 catcacttcc tgattattga cccacacttt gccgtaatga gtgaccgcat cgaaacgcag
    10621 cacgatacgc tggcctgccc aacctttcgg tataaagact tcgcgctgat accagacgtt
    10681 gcccgcataa ttacgaatat ctgcatcggc gaactgatcg ttaaaactgc ctggcacagc
    10741 aattgcccgg ctttcttgta acgcgctttc ccaccaacgc tgatcaattc cacagttttc
    10801 gcgatccaga ctgaatgccc acaggccgtc gagttttttg atttcacggg ttggggtttc
    10861 tacaggacgg accatattta taggtgtaat ttatgtagct gtaattttta ccttattaat
    10921 attttttacg ctttgcattc gacgactgaa ctcccaaata tatgtttaac tcgtcttggt
    10981 cgtttgaatt tttgttgctg tgtttcctaa tattttccat caccttaaat atgttattgt
    11041 aatcctcaat gttgaacttg caattggaca cggcatagtt ttccatagtc gtgtaaaaca
    11101 tggtattggc tgcattgtaa tacatccgac tgagcgggta cggatctatg tgtttgagca
    11161 gcctgttcaa aaactctgca tcgtcgcaaa acggaatttc gtcgacagat cgtaaccaca
    11221 caaaaaacca acacacagtt ctaatgaaaa taaagaactt ttattatcct caggtctagc
    11281 tggaattctt atcaggtttc aggctcgggc acactgacat ccttctcatc gcccgtcttg
    11341 ggaacagctt ccagtaatgg gtccccagga cctgcctcct ctccctcctt ggcctcgctg
    11401 gccttagagt tggggaccca gaggccagag tcaatgcagc gctgcatgtg gtactttgcg
    11461 tcggtggggt ccatcttgct gatggcgtcc tgcagcatct gcacgtcctt cacatcgaag
    11521 cacttctgga gttcctcagg gagggactcg tagacctcga cggggtccag gccgccgggg
    11581 ccgagccgct tcttgcgctc ctcctcctcg tactccttca tggccttctc gatgcgcagc
    11641 ttggcacggc cccgcacacg ctccttgaag gcttccagct cgtcgttgaa gccctccatg
    11701 tactggcgat cggctgtctt aatcttagtg aagaactgcc ggaagcaggc ccgggggtcc
    11761 acctttaggc tcttggccag ctccagg
//
