LOCUS       sequence-52075-e       11892 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11892)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBACgus4x/cdc37/RocCOR LRRK2 1867-2176
FEATURES             Location/Qualifiers
     source          1..11892
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(665..774)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        complement(925..1016)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     promoter        1041..1150
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        1256..1347
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             1366..1389
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             1390..1410
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             4052..4069
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_recomb     4106..5463
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             complement(4122..5463)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     primer_bind     complement(5555..5572)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5726..6314)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5806..5825)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6485..7276,7277..7345))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7108..7127
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7346..7450)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7477..7932
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7564..7583)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7774..7795
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_recomb     8331..9158
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             8331..8455
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(8493..9098)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     CDS             complement(9169..10980)
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGQQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFSLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     promoter        complement(10989..11304)
                     /gene="Autographa californica p6.9"
                     /label=p6.9 promoter
                     /note="baculovirus late basic protein promoter"
ORIGIN
        1 ataaattgca tgacgattgt ctggtgggcc acctgctcca tgagtgcaca tttctcctcc
       61 acctctaggt caatgcacca aatgaccagg taattggctg tctcctcgca caccaggtgg
      121 acgttgtctg acaggtactt ttggctgtca tcccagcggc gaagcatgcc aaagtgcttg
      181 atctgtttct cgtatttttc cacgaaggtc ttgtgtttct gctccctcac ctcctctgag
      241 tcctcctccg tcttctcggg cttggtattt accatgctct tgctgaagcc gtctttgctg
      301 agcgtgtcca cgttccaggg catgctcttc tccttcttgc gcatctcctc cagcttctgc
      361 tcccagctcc gctcctcctt gcgcagctgc tgtgcctcgg cctgcaggcg ctccagctct
      421 gccttgccgc cctcggccac ctccagctcc ttcagtttcc tctggcactc ggccaccttg
      481 cgcttgcact cgcggcagcc cctgtccagt tcctccttct ccttctggaa ctgctccatg
      541 cgttccaccc gggcctgatg ccgccagcgg aagagactgg ccgtgtcgat gttggggtgc
      601 gtctcgtctt catcatcaga cacctcaatg tggtcccaca cgctgtagtc caccatggat
      661 ctgtgattgt aaataaaatg taatttacag tatagtattt taattaatat acaaatgatt
      721 tgataataat tcttatttaa ctataatata ttgtgttggg ttgaattaaa ggtcccggca
      781 tcctcaaatg cataatttca tagtccccct tgttgtaagt gatgcgtatt tctgaatctt
      841 tgtaaaatag cacacaagac tccaacgcgt ttggcgtttt attttcttgc tcgactctag
      901 tttattaggc ctctagagat ccgtatttat aggttttttt attacaaaac tgttacgaaa
      961 acagtaaaat acttatttat ttgcgagatg gttatcattt taattatctc catgatccaa
     1021 taacctagaa taaagggccc gacctttaat tcaacccaac acaatatatt atagttaaat
     1081 aagaattatt atcaaatcat ttgtatatta attaaaatac tatactgtaa attacatttt
     1141 atttacaatc acagatcccc gggactagta ataaaagatg tttattttca ttagatgtgt
     1201 gtgttggttt tttgtgtcta gtaataaaac ctagaggatc gatcccccgg atctgatcat
     1261 ggagataatt aaaatgataa ccatctcgca aataaataag tattttactg ttttcgtaac
     1321 agttttgtaa taaaaaaacc tataaatatc ggatccggat ccatgcacca ccatcaccac
     1381 catcaccacg agaaccttta tttccaagga aattttgact ttaaacatat tgggtgtaaa
     1441 gcaaaagaca ttattaggtt cttgcagcaa cgccttaaga aggcagtgcc gtacaataga
     1501 atgaaactta tgatcgttgg taatacgggt tctggtaaaa cgactctatt acaacagttg
     1561 atgaaaacga agaagagcga tctaggaatg caaagcgcta ccgtcggcat agacgtcaaa
     1621 gattggccta tacaaatacg ggacaaacgc aaacgtgacc tcgtgttgaa cgtttgggac
     1681 tttgctggcc gggaagaatt ttactctaca catccgcatt ttatgactca acgcgctttg
     1741 taccttgcag tgtatgattt gtccaaggga caggctgagg tcgacgcaat gaagccctgg
     1801 ttgtttaata ttaaagcgcg cgcatcatcg tctccggtga ttcttgtcgg aacccatctc
     1861 gatgtgagtg acgagaaaca acgcaaagcg tgcatgagta aaatcacaaa agaattactg
     1921 aataaacgag gttttccggc gatacgcgat tatcactttg tgaatgcaac tgaagaatcc
     1981 gacgctttgg cgaaattgcg caaaaccatt ataaatgaga gcctaaactt taaaatacgc
     2041 gaccaattag tggtgggtca acttattccg gattgctacg tagagttgga aaagataatt
     2101 ctctctgaac gcaagaacgt gcccattgaa tttccggtga tcgaccgtaa acgtcttttg
     2161 caattggtcc gtgaaaatca attgcaattg gacgagaacg aattgcctca cgcggtgcac
     2221 ttcttaaacg agagcggcgt cttgcttcat tttcaggacc cggcgttgca actgagtgac
     2281 ctatacttcg tggaaccgaa atggctatgt aagatcatgg cgcaaattct gactgttaaa
     2341 gttgaaggtt gtcctaaaca tcccaaaggc ataatttcga ggcgagacgt ggagaagttc
     2401 ttgagtaaga aacgtaaatt tccgaagaat tacatgagcc aatactttaa attgttggag
     2461 aaatttcaga ttgcgttgcc gattggagaa gaatacttac tggtcccgag ctcgctgagc
     2521 gaccatcgcc cggttattga actaccgcat tgcgagaaca gcgagataat tatacgcctg
     2581 tacgagatgc cctactttcc catgggcttc tggagcagat tgattaacag attgttggag
     2641 atcagcccct acatgctgtc aggtcgagag cgcgcactac gccccaatcg tatgtattgg
     2701 cggcagggca tatatttgaa ttggtcgccg gaagcatact gtctagtagg ctcagaagtc
     2761 ttagataatc acccggagtc ttttcttaaa attaccgtgc cgagctgccg taaagggtgt
     2821 atcttgttag gccaagtggt cgaccacatt gatagcttga tggaagaatg gtttccaggc
     2881 ctccttgaaa tcgacatttg cggcgagggc gagacactct tgaagaagtg ggcgttgtat
     2941 tcttttaacg atggcgagga gcaccagaag atactgctag atgacctgat gaagaaagcg
     3001 gaagaaggcg acctgttggt gaaccctgac cagccgagat tgacgatacc catttcacaa
     3061 atagcacctg acctaatttt ggccgatttg cctcgcaata ttatgctaaa caacgacgag
     3121 ttagagtttg agcaagctcc cgaatttcta ctaggcgacg gttcttttgg ctcggtatac
     3181 cgagcggctt atgaaggcga agaagtggct gttaagatct tcaacaagca cacctcgctc
     3241 cgtctgctcc gtcaggaact ggtcgttctg tgtcatctgc accatccgag cctcatctcc
     3301 ctgctggccg ctggcatccg tcctcgtatg ctggtgatgg agctggcatc caagggctct
     3361 ctcgaccgcc tgctgcagca ggacaaggct tctctgaccc gtactctgca gcatcgtatc
     3421 gctctgcatg tagctgacgg cctgcgttat cttcattctg caatgatcat ttatcgcgac
     3481 ctgaaacctc ataatgtact gctgtttact ctgtacccga acgctgccat catcgctaag
     3541 attgcagact atggtattgc gcagtactgc tgccgcatgg gcattaaaac aagcgaaggt
     3601 acgccgggtt tccgtgctcc agaagtggct cgtggcaacg ttatttacaa ccagcaagct
     3661 gacgtttact cgttcggtct gctcctgtac gatatcctga ctaccggcgg ccgtatcgta
     3721 gaaggtctga agttccctaa cgaattcgac gaactggaaa tccagggcaa gctgccagac
     3781 ccggttaaag aatacggttg cgctccgtgg ccgatggtag agaagctcat caaacagtgc
     3841 cttaaagaaa accctcagga acgtccaacc tctgctcaag tgttcgacat ccttaacagc
     3901 gctgaactcg tctgtctgac tcgccgtatt ctcctgccta agaatgttat cgtggaatgt
     3961 atggttgcta ctcaccataa ctctcgcaac gcttccatct ggctcggctg tggccacact
     4021 gaccgttgat aagcttgcgg ccgcactcga gcaccaccac caccaccact aacctaggta
     4081 gctgagcgca tgcaagctga tccgggttat tagtacattt attaagcgct agattctgtg
     4141 cgttgttgat ttacagacaa ttgttgtacg tattttaata attcattaaa tttataatct
     4201 ttagggtggt atgttagagc gaaaatcaaa tgattttcag cgtctttata tctgaattta
     4261 aatattaaat cctcaataga tttgtaaaat aggtttcgat tagtttcaaa caagggttgt
     4321 ttttccgaac cgatggctgg actatctaat ggattttcgc tcaacgccac aaaacttgcc
     4381 aaatcttgta gcagcaatct agctttgtcg atattcgttt gtgttttgtt ttgtaataaa
     4441 ggttcgacgt cgttcaaaat attatgcgct tttgtatttc tttcatcact gtcgttagtg
     4501 tacaattgac tcgacgtaaa cacgttaaat agagcttgga catatttaac atcgggcgtg
     4561 ttagctttat taggccgatt atcgtcgtcg tcccaaccct cgtcgttaga agttgcttcc
     4621 gaagacgatt ttgccatagc cacacgacgc ctattaattg tgtcggctaa cacgtccgcg
     4681 atcaaatttg tagttgagct ttttggaatt atttctgatt gcgggcgttt ttgggcgggt
     4741 ttcaatctaa ctgtgcccga ttttaattca gacaacacgt tagaaagcga tggtgcaggc
     4801 ggtggtaaca tttcagacgg caaatctact aatggcggcg gtggtggagc tgatgataaa
     4861 tctaccatcg gtggaggcgc aggcggggct ggcggcggag gcggaggcgg aggtggtggc
     4921 ggtgatgcag acggcggttt aggctcaaat gtctctttag gcaacacagt cggcacctca
     4981 actattgtac tggtttcggg cgccgttttt ggtttgaccg gtctgagacg agtgcgattt
     5041 ttttcgtttc taatagcttc caacaattgt tgtctgtcgt ctaaaggtgc agcgggttga
     5101 ggttccgtcg gcattggtgg agcgggcggc aattcagaca tcgatggtgg tggtggtggt
     5161 ggaggcgctg gaatgttagg cacgggagaa ggtggtggcg gcggtgccgc cggtataatt
     5221 tgttctggtt tagtttgttc gcgcacgatt gtgggcaccg gcgcaggcgc cgctggctgc
     5281 acaacggaag gtcgtctgct tcgaggcagc gcttggggtg gtggcaattc aatattataa
     5341 ttggaataca aatcgtaaaa atctgctata agcattgtaa tttcgctatc gtttaccgtg
     5401 ccgatattta acaaccgctc aatgtaagca attgtattgt aaagagattg tctcaagctc
     5461 ggatcgatcc cgcacgccga taacaagcct tttcattttt actacagcat tgtagtggcg
     5521 agacacttcg ctgtcgtcga ggtttaaacg cttcctcgct cactgactcg ctgcgctcgg
     5581 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     5641 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     5701 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     5761 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     5821 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     5881 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     5941 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     6001 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     6061 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     6121 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     6181 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     6241 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     6301 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     6361 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     6421 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     6481 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     6541 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     6601 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     6661 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     6721 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     6781 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     6841 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     6901 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     6961 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     7021 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     7081 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     7141 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     7201 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     7261 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     7321 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     7381 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     7441 gggttccgcg cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa
     7501 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     7561 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     7621 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     7681 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     7741 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     7801 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     7861 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     7921 cgtttacaat ttcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     7981 gggcctcttc gctattacgc caggaacggc tccgcccact attaatgaaa ttaaaaattc
     8041 caattttaaa aaacgcagca agagaaacat ttgtatgaaa gaatgcgtag aaggaaagaa
     8101 aaatgtcgtc gacatgctga acaacaagat taatatgcct ccgtgtataa aaaaaatatt
     8161 gaacgatttg aaagaaaaca atgtaccgcg cggcggtatg tacaggaaga ggtttatact
     8221 aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat gtttaatcaa
     8281 ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca tgcatctttt
     8341 aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa ctgtcgacaa
     8401 gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt attgaataat
     8461 aaaacaatta taaatgctaa atttgttttt tattaacgat acaaaccaaa cgcaacaaga
     8521 acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag gtaatattta
     8581 aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt ttcacataaa
     8641 ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt ctcttcataa
     8701 aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt aaattttttg
     8761 ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg catagttttt
     8821 ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc tttaattatt
     8881 aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt gccggcatag
     8941 tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt aacataactt
     9001 tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc tatactattg
     9061 tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg cacaaactaa
     9121 tatcacaaac tggaaatgtc tatcaatata tagttgctga tcagatcctc attgtttgcc
     9181 tccctgctgc ggtttttcac cgaagttcat gccagtccag cgtttttgca gcagaaaagc
     9241 cgccgacttc ggtttgcggt cgcgagtgaa gatccctttc ttgttaccgc caacgcgcaa
     9301 tatgccttgc gaggtcgcaa aatcggcgaa attccatacc tgttcaccga cgacggcgct
     9361 gacgcgatca aagacgcggt gatacatatc cagccatgca cactgatact cttcactcca
     9421 catgtcggtg tacattgagt gcagcccggc taacgtatcc acgccgtatt cggtgatgat
     9481 aatcggctga tgcagtttct cctgccaggc cagaagttct ttttccagta ccttctctgc
     9541 cgtttccaaa tcgccgcttt ggacatacca tccgtaataa cggttcaggc acagcacatc
     9601 aaagagatcg ctgatggtat cggtgtgagc gtcgcagaac attacattga cgcaggtgat
     9661 cggacgcgtc gggtcgagtt tacgcgttgc ttccgccagt ggcgcgaaat attcccgtgc
     9721 accttgcgga cgggtatccg gttcgttggc aatactccac atcaccacgc ttgggtggtt
     9781 tttgtcacgc gctatcagct ctttaatcgc ctgtaagtgc gcttgctgag tttccccgtt
     9841 gactgcctct tcgctgtaca gttctttcgg cttgttgccc gcttcgaaac caatgcctaa
     9901 agagagcgaa aagccgacag cagcagtttc atcaatcacc acgatgccat gttcatctgc
     9961 ccagtcgagc atctcttcag cgtaagggta atgcgaggta cggtaggagt tggccccaat
    10021 ccagtccatt aatgcgtggt cgtgcaccat cagcacgtta tcgaatcctt tgccacgtaa
    10081 gtccgcatct tcatgacgac caaagccagt aaagtagaac ggtttgtggt taatcaggaa
    10141 ctgttggccc ttcactgcca ctgaccggat gccgacgcga agcgggtaga tatcacactc
    10201 tgtctggctt ttggctgtga cgcacagttc atagagataa ccttcacccg gttgccagag
    10261 gtgcggattc accacttgca aagtcccgct agtgccttgt ccagttgcaa ccacctgttg
    10321 atccgcatca cgcagttcaa cgctgacatc accattggcc accacctgcc agtcaacaga
    10381 cgcgtggtta cagtcttgcg cgacatgcgt caccacggtg atatcgtcca cccaggtgtt
    10441 cggcgtggtg tagagcatta cgctgcgatg gattccggca tagttaaaga aatcatggaa
    10501 gtaagactgc tttttcttgc cgttttcgtc ggtaatcacc attcccggcg ggatagtctg
    10561 ccagttcagt tcgttgttca cacaaacggt gatacgtaca cttttcccgg caataacata
    10621 cggcgtgaca tcggcttcaa atggcgtata gccgccctga tgctccatca cttcctgatt
    10681 attgacccac actttgccgt aatgagtgac cgcatcgaaa cgcagcacga tacgctggcc
    10741 tgcccaacct ttcggtataa agacttcgcg ctgataccag acgttgcccg cataattacg
    10801 aatatctgca tcggcgaact gatcgttaaa actgcctggc acagcaattg cccggctttc
    10861 ttgtaacgcg ctttcccacc aacgctgatc aattccacag ttttcgcgat ccagactgaa
    10921 tgcccacagg ccgtcgagtt ttttgatttc acgggttggg gtttctacag gacggaccat
    10981 atttataggt gtaatttatg tagctgtaat ttttacctta ttaatatttt ttacgctttg
    11041 cattcgacga ctgaactccc aaatatatgt ttaactcgtc ttggtcgttt gaatttttgt
    11101 tgctgtgttt cctaatattt tccatcacct taaatatgtt attgtaatcc tcaatgttga
    11161 acttgcaatt ggacacggca tagttttcca tagtcgtgta aaacatggta ttggctgcat
    11221 tgtaatacat ccgactgagc gggtacggat ctatgtgttt gagcagcctg ttcaaaaact
    11281 ctgcatcgtc gcaaaacgga atttcgtcga cagatcgtaa ccacacaaaa aaccaacaca
    11341 cagttctaat gaaaataaag aacttttatt atcctcaggt ctagctggaa ttcttatcag
    11401 gtttcaggct cgggcacact gacatccttc tcatcgcccg tcttgggaac agcttccagt
    11461 aatgggtccc caggacctgc ctcctctccc tccttggcct cgctggcctt agagttgggg
    11521 acccagaggc cagagtcaat gcagcgctgc atgtggtact ttgcgtcggt ggggtccatc
    11581 ttgctgatgg cgtcctgcag catctgcacg tccttcacat cgaagcactt ctggagttcc
    11641 tcagggaggg actcgtagac ctcgacgggg tccaggccgc cggggccgag ccgcttcttg
    11701 cgctcctcct cctcgtactc cttcatggcc ttctcgatgc gcagcttggc acggccccgc
    11761 acacgctcct tgaaggcttc cagctcgtcg ttgaagccct ccatgtactg gcgatcggct
    11821 gtcttaatct tagtgaagaa ctgccggaag caggcccggg ggtccacctt taggctcttg
    11881 gccagctcca gg
//
