LOCUS       sequence-52076-e        7633 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7633)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBACgus N-His/GST
FEATURES             Location/Qualifiers
     source          1..7633
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             7..660
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     CDS             733..753
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             800..817
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_recomb     854..2211
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             complement(870..2211)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     primer_bind     complement(2303..2320)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2474..3062)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2554..2573)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3233..4024,4025..4093))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3856..3875
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4094..4198)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4225..4680
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4312..4331)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4522..4543
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_recomb     5079..5906
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             5079..5203
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(5241..5846)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
ORIGIN
        1 ggatcaatgg caccgatcct ggggtattgg aagatcaagg gcctagtgca gcccactcgt
       61 ctgctcctgg agtacctaga agagaagtat gaggagcacc tttatgagcg ggacgagggt
      121 gacaagtggc gcaataagaa attcgagcta ggcttggaat tccccaatct gccttattac
      181 atcgatggcg acgttaagtt gactcagtcc atggccatca tccgctacat cgccgacaag
      241 cacaatatgc taggcgggtg cccgaaggag cgagctgaga tctctatgtt ggaaggggcc
      301 gtgctggata tccgctacgg cgtgagccgc attgcatact caaaggattt cgagaccctc
      361 aaggttgact tcctgtcaaa gctgcctgaa atgctaaaga tgttcgaaga ccgtctctgt
      421 cataagacct acctgaacgg ggaccacgtg acacacccgg actttatgtt gtatgacgct
      481 ctggacgtcg tgctttatat ggaccctatg tgcctggacg cttttcccaa actagtgtgt
      541 ttcaagaaac gtatcgaagc tatccctcag attgataagt acctgaagtc ctcaaagtac
      601 atcgcctggc cccttcaagg ttggcaagcc acatttggcg gtggcgacca tcctcccaag
      661 tcagacctag tgcctaggca caaccagacc agcctatata agaaagccgg gtcagctgcc
      721 gctgtgctgg aggagaacct gtatttccag ggcggatcca ggcctgaatt cgagctccaa
      781 gctttagtaa gcactcgagc accaccacca ccaccactaa cctaggtagc tgagcgcatg
      841 caagctgatc cgggttatta gtacatttat taagcgctag attctgtgcg ttgttgattt
      901 acagacaatt gttgtacgta ttttaataat tcattaaatt tataatcttt agggtggtat
      961 gttagagcga aaatcaaatg attttcagcg tctttatatc tgaatttaaa tattaaatcc
     1021 tcaatagatt tgtaaaatag gtttcgatta gtttcaaaca agggttgttt ttccgaaccg
     1081 atggctggac tatctaatgg attttcgctc aacgccacaa aacttgccaa atcttgtagc
     1141 agcaatctag ctttgtcgat attcgtttgt gttttgtttt gtaataaagg ttcgacgtcg
     1201 ttcaaaatat tatgcgcttt tgtatttctt tcatcactgt cgttagtgta caattgactc
     1261 gacgtaaaca cgttaaatag agcttggaca tatttaacat cgggcgtgtt agctttatta
     1321 ggccgattat cgtcgtcgtc ccaaccctcg tcgttagaag ttgcttccga agacgatttt
     1381 gccatagcca cacgacgcct attaattgtg tcggctaaca cgtccgcgat caaatttgta
     1441 gttgagcttt ttggaattat ttctgattgc gggcgttttt gggcgggttt caatctaact
     1501 gtgcccgatt ttaattcaga caacacgtta gaaagcgatg gtgcaggcgg tggtaacatt
     1561 tcagacggca aatctactaa tggcggcggt ggtggagctg atgataaatc taccatcggt
     1621 ggaggcgcag gcggggctgg cggcggaggc ggaggcggag gtggtggcgg tgatgcagac
     1681 ggcggtttag gctcaaatgt ctctttaggc aacacagtcg gcacctcaac tattgtactg
     1741 gtttcgggcg ccgtttttgg tttgaccggt ctgagacgag tgcgattttt ttcgtttcta
     1801 atagcttcca acaattgttg tctgtcgtct aaaggtgcag cgggttgagg ttccgtcggc
     1861 attggtggag cgggcggcaa ttcagacatc gatggtggtg gtggtggtgg aggcgctgga
     1921 atgttaggca cgggagaagg tggtggcggc ggtgccgccg gtataatttg ttctggttta
     1981 gtttgttcgc gcacgattgt gggcaccggc gcaggcgccg ctggctgcac aacggaaggt
     2041 cgtctgcttc gaggcagcgc ttggggtggt ggcaattcaa tattataatt ggaatacaaa
     2101 tcgtaaaaat ctgctataag cattgtaatt tcgctatcgt ttaccgtgcc gatatttaac
     2161 aaccgctcaa tgtaagcaat tgtattgtaa agagattgtc tcaagctcgg atcgatcccg
     2221 cacgccgata acaagccttt tcatttttac tacagcattg tagtggcgag acacttcgct
     2281 gtcgtcgagg tttaaacgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     2341 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     2401 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     2461 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     2521 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     2581 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     2641 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     2701 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     2761 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     2821 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     2881 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     2941 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     3001 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     3061 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     3121 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     3181 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     3241 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     3301 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     3361 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     3421 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     3481 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     3541 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     3601 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     3661 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     3721 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     3781 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     3841 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     3901 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     3961 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     4021 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     4081 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     4141 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     4201 catttccccg aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     4261 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     4321 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     4381 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     4441 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     4501 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     4561 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     4621 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt
     4681 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     4741 tattacgcca ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa
     4801 acgcagcaag agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga
     4861 catgctgaac aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa
     4921 agaaaacaat gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat
     4981 tgcaaacgtg gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca
     5041 tttctacaac cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca
     5101 agatgtgtat aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt
     5161 tgctggcaac tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata
     5221 aatgctaaat ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta
     5281 ttatctataa ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca
     5341 aatgattcac agttaatttg cgacaatata attttatttt cacataaact agacgccttg
     5401 tcgtcttctt cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag
     5461 ttattatcgt atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata
     5521 tatatgtctt ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca
     5581 acagtgctat tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa
     5641 tcaatgaatt tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct
     5701 tctagttcaa ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg
     5761 tacgaaccgt taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt
     5821 tgtttgttgt taaaaataac agccattgta atgagacgca caaactaata tcacaaactg
     5881 gaaatgtcta tcaatatata gttgctgatc agatcctcat tgtttgcctc cctgctgcgg
     5941 tttttcaccg aagttcatgc cagtccagcg tttttgcagc agaaaagccg ccgacttcgg
     6001 tttgcggtcg cgagtgaaga tccctttctt gttaccgcca acgcgcaata tgccttgcga
     6061 ggtcgcaaaa tcggcgaaat tccatacctg ttcaccgacg acggcgctga cgcgatcaaa
     6121 gacgcggtga tacatatcca gccatgcaca ctgatactct tcactccaca tgtcggtgta
     6181 cattgagtgc agcccggcta acgtatccac gccgtattcg gtgatgataa tcggctgatg
     6241 cagtttctcc tgccaggcca gaagttcttt ttccagtacc ttctctgccg tttccaaatc
     6301 gccgctttgg acataccatc cgtaataacg gttcaggcac agcacatcaa agagatcgct
     6361 gatggtatcg gtgtgagcgt cgcagaacat tacattgacg caggtgatcg gacgcgtcgg
     6421 gtcgagttta cgcgttgctt ccgccagtgg cgcgaaatat tcccgtgcac cttgcggacg
     6481 ggtatccggt tcgttggcaa tactccacat caccacgctt gggtggtttt tgtcacgcgc
     6541 tatcagctct ttaatcgcct gtaagtgcgc ttgctgagtt tccccgttga ctgcctcttc
     6601 gctgtacagt tctttcggct tgttgcccgc ttcgaaacca atgcctaaag agagcgaaaa
     6661 gccgacagca gcagtttcat caatcaccac gatgccatgt tcatctgccc agtcgagcat
     6721 ctcttcagcg taagggtaat gcgaggtacg gtaggagttg gccccaatcc agtccattaa
     6781 tgcgtggtcg tgcaccatca gcacgttatc gaatcctttg ccacgtaagt ccgcatcttc
     6841 atgacgacca aagccagtaa agtagaacgg tttgtggtta atcaggaact gttggccctt
     6901 cactgccact gaccggatgc cgacgcgaag cgggtagata tcacactctg tctggctttt
     6961 ggctgtgacg cacagttcat agagataacc ttcacccggt tgccagaggt gcggattcac
     7021 cacttgcaaa gtcccgctag tgccttgtcc agttgcaacc acctgttgat ccgcatcacg
     7081 cagttcaacg ctgacatcac cattggccac cacctgccag tcaacagacg cgtggttaca
     7141 gtcttgcgcg acatgcgtca ccacggtgat atcgtccacc caggtgttcg gcgtggtgta
     7201 gagcattacg ctgcgatgga ttccggcata gttaaagaaa tcatggaagt aagactgctt
     7261 tttcttgccg ttttcgtcgg taatcaccat tcccggcggg atagtctgcc agttcagttc
     7321 gttgttcaca caaacggtga tacgtacact tttcccggca ataacatacg gcgtgacatc
     7381 ggcttcaaat ggcgtatagc cgccctgatg ctccatcact tcctgattat tgacccacac
     7441 tttgccgtaa tgagtgaccg catcgaaacg cagcacgata cgctggcctg cccaaccttt
     7501 cggtataaag acttcgcgct gataccagac gttgcccgca taattacgaa tatctgcatc
     7561 ggcgaactga tcgttaaaac tgcctggcac agcaattgcc cggctttctt gtaacgcgct
     7621 ttcccaccaa cgc
//
