LOCUS       sequence-52079-e       12138 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12138)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12138)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBACgus4x/Parkin/RocCOR LRRK2 1867-2176
FEATURES             Location/Qualifiers
     source          1..12138
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     329..1156
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             329..453
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(491..1096)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     CDS             complement(1167..2978)
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGQQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFSLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     promoter        complement(2987..3302)
                     /gene="Autographa californica p6.9"
                     /label=p6.9 promoter
                     /note="baculovirus late basic protein promoter"
     promoter        complement(4801..4910)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        complement(5061..5152)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     promoter        5177..5286
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        5392..5483
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             5502..5525
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             5526..5546
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             8188..8205
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_recomb     8242..9599
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             complement(8258..9599)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     primer_bind     complement(9691..9708)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9862..10450)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9942..9961)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10621..11412,11413..11481))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11244..11263
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11482..11586)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      11613..12068
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11700..11719)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11910..11931
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag
       61 agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac
      121 aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat
      181 gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg
      241 gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac
      301 cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat
      361 aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac
      421 tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat
      481 ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa
      541 ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac
      601 agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt
      661 cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt
      721 atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt
      781 ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat
      841 tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt
      901 tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa
      961 ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt
     1021 taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt
     1081 taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta
     1141 tcaatatata gttgctgatc agatcctcat tgtttgcctc cctgctgcgg tttttcaccg
     1201 aagttcatgc cagtccagcg tttttgcagc agaaaagccg ccgacttcgg tttgcggtcg
     1261 cgagtgaaga tccctttctt gttaccgcca acgcgcaata tgccttgcga ggtcgcaaaa
     1321 tcggcgaaat tccatacctg ttcaccgacg acggcgctga cgcgatcaaa gacgcggtga
     1381 tacatatcca gccatgcaca ctgatactct tcactccaca tgtcggtgta cattgagtgc
     1441 agcccggcta acgtatccac gccgtattcg gtgatgataa tcggctgatg cagtttctcc
     1501 tgccaggcca gaagttcttt ttccagtacc ttctctgccg tttccaaatc gccgctttgg
     1561 acataccatc cgtaataacg gttcaggcac agcacatcaa agagatcgct gatggtatcg
     1621 gtgtgagcgt cgcagaacat tacattgacg caggtgatcg gacgcgtcgg gtcgagttta
     1681 cgcgttgctt ccgccagtgg cgcgaaatat tcccgtgcac cttgcggacg ggtatccggt
     1741 tcgttggcaa tactccacat caccacgctt gggtggtttt tgtcacgcgc tatcagctct
     1801 ttaatcgcct gtaagtgcgc ttgctgagtt tccccgttga ctgcctcttc gctgtacagt
     1861 tctttcggct tgttgcccgc ttcgaaacca atgcctaaag agagcgaaaa gccgacagca
     1921 gcagtttcat caatcaccac gatgccatgt tcatctgccc agtcgagcat ctcttcagcg
     1981 taagggtaat gcgaggtacg gtaggagttg gccccaatcc agtccattaa tgcgtggtcg
     2041 tgcaccatca gcacgttatc gaatcctttg ccacgtaagt ccgcatcttc atgacgacca
     2101 aagccagtaa agtagaacgg tttgtggtta atcaggaact gttggccctt cactgccact
     2161 gaccggatgc cgacgcgaag cgggtagata tcacactctg tctggctttt ggctgtgacg
     2221 cacagttcat agagataacc ttcacccggt tgccagaggt gcggattcac cacttgcaaa
     2281 gtcccgctag tgccttgtcc agttgcaacc acctgttgat ccgcatcacg cagttcaacg
     2341 ctgacatcac cattggccac cacctgccag tcaacagacg cgtggttaca gtcttgcgcg
     2401 acatgcgtca ccacggtgat atcgtccacc caggtgttcg gcgtggtgta gagcattacg
     2461 ctgcgatgga ttccggcata gttaaagaaa tcatggaagt aagactgctt tttcttgccg
     2521 ttttcgtcgg taatcaccat tcccggcggg atagtctgcc agttcagttc gttgttcaca
     2581 caaacggtga tacgtacact tttcccggca ataacatacg gcgtgacatc ggcttcaaat
     2641 ggcgtatagc cgccctgatg ctccatcact tcctgattat tgacccacac tttgccgtaa
     2701 tgagtgaccg catcgaaacg cagcacgata cgctggcctg cccaaccttt cggtataaag
     2761 acttcgcgct gataccagac gttgcccgca taattacgaa tatctgcatc ggcgaactga
     2821 tcgttaaaac tgcctggcac agcaattgcc cggctttctt gtaacgcgct ttcccaccaa
     2881 cgctgatcaa ttccacagtt ttcgcgatcc agactgaatg cccacaggcc gtcgagtttt
     2941 ttgatttcac gggttggggt ttctacagga cggaccatat ttataggtgt aatttatgta
     3001 gctgtaattt ttaccttatt aatatttttt acgctttgca ttcgacgact gaactcccaa
     3061 atatatgttt aactcgtctt ggtcgtttga atttttgttg ctgtgtttcc taatattttc
     3121 catcacctta aatatgttat tgtaatcctc aatgttgaac ttgcaattgg acacggcata
     3181 gttttccata gtcgtgtaaa acatggtatt ggctgcattg taatacatcc gactgagcgg
     3241 gtacggatct atgtgtttga gcagcctgtt caaaaactct gcatcgtcgc aaaacggaat
     3301 ttcgtcgaca gatcgtaacc acacaaaaaa ccaacacaca gttctaatga aaataaagaa
     3361 cttttattat cctcaggtct agctggaatt cttatcacac gtcaaaccag tgatcaccca
     3421 tacacacgcg gttccactca cacccacagt tccaacacca ctccagccga cactgtggct
     3481 gtgggcactt catgtgcata cacccgccgt ttttttccac aggcacatgg cacctaggac
     3541 agggcttggt ggtcttctta atagtctcct ttgaagcggc ctcccaacgt gcctgctctg
     3601 ctgctcgctc gtcaacacgg taggcctgtg tggtcgtccc tgatgcctcg aacacggcag
     3661 aacactcgcc ctcgtggtat gcttccttac attcgcggca gaaggcgaac ccgcagccta
     3721 gcccgttacc accttcacag gtcaccttgc gctggtctgg ctctggcaag aggcctgctc
     3781 cacaaccagg cctagggcag agtacgccgc ccatctgtag cacgcactcc tcagcgccgt
     3841 actgttgata acggttgtac tgctcttcgc ctagtatccg aaagtggtgg agttctttaa
     3901 tcaggctatt gggacagcca gcgacacaag gcaggctgta gccgagctga gggtcgtgga
     3961 cgaactggcg gtcattcagg cgtgtgacac aatacagatg gaagcagtcc agacagatga
     4021 cgtgacggct attgcactgg aaaactagca cgggtgatcg cacgtcggtg cacgtgatac
     4081 aagtgatgtt acgggagttg gtagcaatca ggtgcaaggc tactggggtc tccttgtcgg
     4141 aggtagggtg ggcgccacat ttaaagaaga actctgcaga ggtaccaggg caatgaggtg
     4201 actgacactc accgctcatg cggttaggga tgagcacatc gtcccaacaa gacgggccct
     4261 gcgtcagtgt cagggtagcc tggcgacaag tgctacactg caccctcagc tttcctggct
     4321 gcacacgttg gcaagggcct ttacagtaca cgtagaagct gttgtagata ctacggccgg
     4381 ctgggctacc tgcgggagga gaatccttgc gtgagtcggt atgaaggatc acagccagac
     4441 ccacggagtc tcccggcaga accgagctgg acaggtcaac gcgtgtcagg gactgaggct
     4501 cgcgttcaca accgccagcg gcgttgcgtg ggtcgtcccc tccggtagcg ttcatctctt
     4561 gacccttacg ccaaggacgc tgcacgatgt gcactatgct ctgttggtca aggtcgcaat
     4621 tctgcacggt ccagtcgttg cggagttctt ttccggcgaa gatcacgcgc agctggtcag
     4681 cagggacgcc ctgacgtttg gcgacgacct ccttcagctg gaagatgctc gtgtcagagt
     4741 ccacctctac agggaacccg tgcgaggagt tgaagcgcac gaaaacaatc atagatctgt
     4801 gattgtaaat aaaatgtaat ttacagtata gtattttaat taatatacaa atgatttgat
     4861 aataattctt atttaactat aatatattgt gttgggttga attaaaggtc ccggcatcct
     4921 caaatgcata atttcatagt cccccttgtt gtaagtgatg cgtatttctg aatctttgta
     4981 aaatagcaca caagactcca acgcgtttgg cgttttattt tcttgctcga ctctagttta
     5041 ttaggcctct agagatccgt atttataggt ttttttatta caaaactgtt acgaaaacag
     5101 taaaatactt atttatttgc gagatggtta tcattttaat tatctccatg atccaataac
     5161 ctagaataaa gggcccgacc tttaattcaa cccaacacaa tatattatag ttaaataaga
     5221 attattatca aatcatttgt atattaatta aaatactata ctgtaaatta cattttattt
     5281 acaatcacag atccccggga ctagtaataa aagatgttta ttttcattag atgtgtgtgt
     5341 tggttttttg tgtctagtaa taaaacctag aggatcgatc ccccggatct gatcatggag
     5401 ataattaaaa tgataaccat ctcgcaaata aataagtatt ttactgtttt cgtaacagtt
     5461 ttgtaataaa aaaacctata aatatcggat ccggatccat gcaccaccat caccaccatc
     5521 accacgagaa cctttatttc caaggaaatt ttgactttaa acatattggg tgtaaagcaa
     5581 aagacattat taggttcttg cagcaacgcc ttaagaaggc agtgccgtac aatagaatga
     5641 aacttatgat cgttggtaat acgggttctg gtaaaacgac tctattacaa cagttgatga
     5701 aaacgaagaa gagcgatcta ggaatgcaaa gcgctaccgt cggcatagac gtcaaagatt
     5761 ggcctataca aatacgggac aaacgcaaac gtgacctcgt gttgaacgtt tgggactttg
     5821 ctggccggga agaattttac tctacacatc cgcattttat gactcaacgc gctttgtacc
     5881 ttgcagtgta tgatttgtcc aagggacagg ctgaggtcga cgcaatgaag ccctggttgt
     5941 ttaatattaa agcgcgcgca tcatcgtctc cggtgattct tgtcggaacc catctcgatg
     6001 tgagtgacga gaaacaacgc aaagcgtgca tgagtaaaat cacaaaagaa ttactgaata
     6061 aacgaggttt tccggcgata cgcgattatc actttgtgaa tgcaactgaa gaatccgacg
     6121 ctttggcgaa attgcgcaaa accattataa atgagagcct aaactttaaa atacgcgacc
     6181 aattagtggt gggtcaactt attccggatt gctacgtaga gttggaaaag ataattctct
     6241 ctgaacgcaa gaacgtgccc attgaatttc cggtgatcga ccgtaaacgt cttttgcaat
     6301 tggtccgtga aaatcaattg caattggacg agaacgaatt gcctcacgcg gtgcacttct
     6361 taaacgagag cggcgtcttg cttcattttc aggacccggc gttgcaactg agtgacctat
     6421 acttcgtgga accgaaatgg ctatgtaaga tcatggcgca aattctgact gttaaagttg
     6481 aaggttgtcc taaacatccc aaaggcataa tttcgaggcg agacgtggag aagttcttga
     6541 gtaagaaacg taaatttccg aagaattaca tgagccaata ctttaaattg ttggagaaat
     6601 ttcagattgc gttgccgatt ggagaagaat acttactggt cccgagctcg ctgagcgacc
     6661 atcgcccggt tattgaacta ccgcattgcg agaacagcga gataattata cgcctgtacg
     6721 agatgcccta ctttcccatg ggcttctgga gcagattgat taacagattg ttggagatca
     6781 gcccctacat gctgtcaggt cgagagcgcg cactacgccc caatcgtatg tattggcggc
     6841 agggcatata tttgaattgg tcgccggaag catactgtct agtaggctca gaagtcttag
     6901 ataatcaccc ggagtctttt cttaaaatta ccgtgccgag ctgccgtaaa gggtgtatct
     6961 tgttaggcca agtggtcgac cacattgata gcttgatgga agaatggttt ccaggcctcc
     7021 ttgaaatcga catttgcggc gagggcgaga cactcttgaa gaagtgggcg ttgtattctt
     7081 ttaacgatgg cgaggagcac cagaagatac tgctagatga cctgatgaag aaagcggaag
     7141 aaggcgacct gttggtgaac cctgaccagc cgagattgac gatacccatt tcacaaatag
     7201 cacctgacct aattttggcc gatttgcctc gcaatattat gctaaacaac gacgagttag
     7261 agtttgagca agctcccgaa tttctactag gcgacggttc ttttggctcg gtataccgag
     7321 cggcttatga aggcgaagaa gtggctgtta agatcttcaa caagcacacc tcgctccgtc
     7381 tgctccgtca ggaactggtc gttctgtgtc atctgcacca tccgagcctc atctccctgc
     7441 tggccgctgg catccgtcct cgtatgctgg tgatggagct ggcatccaag ggctctctcg
     7501 accgcctgct gcagcaggac aaggcttctc tgacccgtac tctgcagcat cgtatcgctc
     7561 tgcatgtagc tgacggcctg cgttatcttc attctgcaat gatcatttat cgcgacctga
     7621 aacctcataa tgtactgctg tttactctgt acccgaacgc tgccatcatc gctaagattg
     7681 cagactatgg tattgcgcag tactgctgcc gcatgggcat taaaacaagc gaaggtacgc
     7741 cgggtttccg tgctccagaa gtggctcgtg gcaacgttat ttacaaccag caagctgacg
     7801 tttactcgtt cggtctgctc ctgtacgata tcctgactac cggcggccgt atcgtagaag
     7861 gtctgaagtt ccctaacgaa ttcgacgaac tggaaatcca gggcaagctg ccagacccgg
     7921 ttaaagaata cggttgcgct ccgtggccga tggtagagaa gctcatcaaa cagtgcctta
     7981 aagaaaaccc tcaggaacgt ccaacctctg ctcaagtgtt cgacatcctt aacagcgctg
     8041 aactcgtctg tctgactcgc cgtattctcc tgcctaagaa tgttatcgtg gaatgtatgg
     8101 ttgctactca ccataactct cgcaacgctt ccatctggct cggctgtggc cacactgacc
     8161 gttgataagc ttgcggccgc actcgagcac caccaccacc accactaacc taggtagctg
     8221 agcgcatgca agctgatccg ggttattagt acatttatta agcgctagat tctgtgcgtt
     8281 gttgatttac agacaattgt tgtacgtatt ttaataattc attaaattta taatctttag
     8341 ggtggtatgt tagagcgaaa atcaaatgat tttcagcgtc tttatatctg aatttaaata
     8401 ttaaatcctc aatagatttg taaaataggt ttcgattagt ttcaaacaag ggttgttttt
     8461 ccgaaccgat ggctggacta tctaatggat tttcgctcaa cgccacaaaa cttgccaaat
     8521 cttgtagcag caatctagct ttgtcgatat tcgtttgtgt tttgttttgt aataaaggtt
     8581 cgacgtcgtt caaaatatta tgcgcttttg tatttctttc atcactgtcg ttagtgtaca
     8641 attgactcga cgtaaacacg ttaaatagag cttggacata tttaacatcg ggcgtgttag
     8701 ctttattagg ccgattatcg tcgtcgtccc aaccctcgtc gttagaagtt gcttccgaag
     8761 acgattttgc catagccaca cgacgcctat taattgtgtc ggctaacacg tccgcgatca
     8821 aatttgtagt tgagcttttt ggaattattt ctgattgcgg gcgtttttgg gcgggtttca
     8881 atctaactgt gcccgatttt aattcagaca acacgttaga aagcgatggt gcaggcggtg
     8941 gtaacatttc agacggcaaa tctactaatg gcggcggtgg tggagctgat gataaatcta
     9001 ccatcggtgg aggcgcaggc ggggctggcg gcggaggcgg aggcggaggt ggtggcggtg
     9061 atgcagacgg cggtttaggc tcaaatgtct ctttaggcaa cacagtcggc acctcaacta
     9121 ttgtactggt ttcgggcgcc gtttttggtt tgaccggtct gagacgagtg cgattttttt
     9181 cgtttctaat agcttccaac aattgttgtc tgtcgtctaa aggtgcagcg ggttgaggtt
     9241 ccgtcggcat tggtggagcg ggcggcaatt cagacatcga tggtggtggt ggtggtggag
     9301 gcgctggaat gttaggcacg ggagaaggtg gtggcggcgg tgccgccggt ataatttgtt
     9361 ctggtttagt ttgttcgcgc acgattgtgg gcaccggcgc aggcgccgct ggctgcacaa
     9421 cggaaggtcg tctgcttcga ggcagcgctt ggggtggtgg caattcaata ttataattgg
     9481 aatacaaatc gtaaaaatct gctataagca ttgtaatttc gctatcgttt accgtgccga
     9541 tatttaacaa ccgctcaatg taagcaattg tattgtaaag agattgtctc aagctcggat
     9601 cgatcccgca cgccgataac aagccttttc atttttacta cagcattgta gtggcgagac
     9661 acttcgctgt cgtcgaggtt taaacgcttc ctcgctcact gactcgctgc gctcggtcgt
     9721 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     9781 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     9841 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     9901 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     9961 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
    10021 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
    10081 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
    10141 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
    10201 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
    10261 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
    10321 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
    10381 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
    10441 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
    10501 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
    10561 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
    10621 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
    10681 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
    10741 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
    10801 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
    10861 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
    10921 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
    10981 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
    11041 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
    11101 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    11161 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    11221 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    11281 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    11341 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    11401 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    11461 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    11521 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    11581 tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc
    11641 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
    11701 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
    11761 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
    11821 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
    11881 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
    11941 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
    12001 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
    12061 tacaatttcc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
    12121 ctcttcgcta ttacgcca
//
