LOCUS       sequence-52098-e        9085 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9085)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: LRRK2_1867_2161_F1908A_L1936R_I2131Q
FEATURES             Location/Qualifiers
     source          1..9085
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     329..1156
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             329..453
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(491..1096)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     CDS             complement(1167..2978)
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGQQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFSLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     promoter        complement(2987..3302)
                     /gene="Autographa californica p6.9"
                     /label=p6.9 promoter
                     /note="baculovirus late basic protein promoter"
     promoter        3317..3408
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     3333..3350
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             3421..3444
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             3445..3465
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             3469..4122
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     CDS             4195..4215
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             5135..5152
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_recomb     5189..6546
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             complement(5205..6546)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     primer_bind     complement(6638..6655)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6809..7397)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6889..6908)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7568..8359,8360..8428))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8191..8210
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8429..8533)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8560..9015
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8647..8666)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8857..8878
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag
       61 agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac
      121 aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat
      181 gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg
      241 gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac
      301 cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat
      361 aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac
      421 tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat
      481 ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa
      541 ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac
      601 agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt
      661 cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt
      721 atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt
      781 ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat
      841 tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt
      901 tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa
      961 ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt
     1021 taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt
     1081 taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta
     1141 tcaatatata gttgctgatc agatcctcat tgtttgcctc cctgctgcgg tttttcaccg
     1201 aagttcatgc cagtccagcg tttttgcagc agaaaagccg ccgacttcgg tttgcggtcg
     1261 cgagtgaaga tccctttctt gttaccgcca acgcgcaata tgccttgcga ggtcgcaaaa
     1321 tcggcgaaat tccatacctg ttcaccgacg acggcgctga cgcgatcaaa gacgcggtga
     1381 tacatatcca gccatgcaca ctgatactct tcactccaca tgtcggtgta cattgagtgc
     1441 agcccggcta acgtatccac gccgtattcg gtgatgataa tcggctgatg cagtttctcc
     1501 tgccaggcca gaagttcttt ttccagtacc ttctctgccg tttccaaatc gccgctttgg
     1561 acataccatc cgtaataacg gttcaggcac agcacatcaa agagatcgct gatggtatcg
     1621 gtgtgagcgt cgcagaacat tacattgacg caggtgatcg gacgcgtcgg gtcgagttta
     1681 cgcgttgctt ccgccagtgg cgcgaaatat tcccgtgcac cttgcggacg ggtatccggt
     1741 tcgttggcaa tactccacat caccacgctt gggtggtttt tgtcacgcgc tatcagctct
     1801 ttaatcgcct gtaagtgcgc ttgctgagtt tccccgttga ctgcctcttc gctgtacagt
     1861 tctttcggct tgttgcccgc ttcgaaacca atgcctaaag agagcgaaaa gccgacagca
     1921 gcagtttcat caatcaccac gatgccatgt tcatctgccc agtcgagcat ctcttcagcg
     1981 taagggtaat gcgaggtacg gtaggagttg gccccaatcc agtccattaa tgcgtggtcg
     2041 tgcaccatca gcacgttatc gaatcctttg ccacgtaagt ccgcatcttc atgacgacca
     2101 aagccagtaa agtagaacgg tttgtggtta atcaggaact gttggccctt cactgccact
     2161 gaccggatgc cgacgcgaag cgggtagata tcacactctg tctggctttt ggctgtgacg
     2221 cacagttcat agagataacc ttcacccggt tgccagaggt gcggattcac cacttgcaaa
     2281 gtcccgctag tgccttgtcc agttgcaacc acctgttgat ccgcatcacg cagttcaacg
     2341 ctgacatcac cattggccac cacctgccag tcaacagacg cgtggttaca gtcttgcgcg
     2401 acatgcgtca ccacggtgat atcgtccacc caggtgttcg gcgtggtgta gagcattacg
     2461 ctgcgatgga ttccggcata gttaaagaaa tcatggaagt aagactgctt tttcttgccg
     2521 ttttcgtcgg taatcaccat tcccggcggg atagtctgcc agttcagttc gttgttcaca
     2581 caaacggtga tacgtacact tttcccggca ataacatacg gcgtgacatc ggcttcaaat
     2641 ggcgtatagc cgccctgatg ctccatcact tcctgattat tgacccacac tttgccgtaa
     2701 tgagtgaccg catcgaaacg cagcacgata cgctggcctg cccaaccttt cggtataaag
     2761 acttcgcgct gataccagac gttgcccgca taattacgaa tatctgcatc ggcgaactga
     2821 tcgttaaaac tgcctggcac agcaattgcc cggctttctt gtaacgcgct ttcccaccaa
     2881 cgctgatcaa ttccacagtt ttcgcgatcc agactgaatg cccacaggcc gtcgagtttt
     2941 ttgatttcac gggttggggt ttctacagga cggaccatat ttataggtgt aatttatgta
     3001 gctgtaattt ttaccttatt aatatttttt acgctttgca ttcgacgact gaactcccaa
     3061 atatatgttt aactcgtctt ggtcgtttga atttttgttg ctgtgtttcc taatattttc
     3121 catcacctta aatatgttat tgtaatcctc aatgttgaac ttgcaattgg acacggcata
     3181 gttttccata gtcgtgtaaa acatggtatt ggctgcattg taatacatcc gactgagcgg
     3241 gtacggatct atgtgtttga gcagcctgtt caaaaactct gcatcgtcgc aaaacggaat
     3301 ttcgtcgaca gatctgatca tggagataat taaaatgata accatctcgc aaataaataa
     3361 gtattttact gttttcgtaa cagttttgta ataaaaaaac ctataaatat aggatctatg
     3421 caccaccatc accaccatca ccacgagaac ctttatttcc aaggatcaat ggcaccgatc
     3481 ctggggtatt ggaagatcaa gggcctagtg cagcccactc gtctgctcct ggagtaccta
     3541 gaagagaagt atgaggagca cctttatgag cgggacgagg gtgacaagtg gcgcaataag
     3601 aaattcgagc taggcttgga attccccaat ctgccttatt acatcgatgg cgacgttaag
     3661 ttgactcagt ccatggccat catccgctac atcgccgaca agcacaatat gctaggcggg
     3721 tgcccgaagg agcgagctga gatctctatg ttggaagggg ccgtgctgga tatccgctac
     3781 ggcgtgagcc gcattgcata ctcaaaggat ttcgagaccc tcaaggttga cttcctgtca
     3841 aagctgcctg aaatgctaaa gatgttcgaa gaccgtctct gtcataagac ctacctgaac
     3901 ggggaccacg tgacacaccc ggactttatg ttgtatgacg ctctggacgt cgtgctttat
     3961 atggacccta tgtgcctgga cgcttttccc aaactagtgt gtttcaagaa acgtatcgaa
     4021 gctatccctc agattgataa gtacctgaag tcctcaaagt acatcgcctg gccccttcaa
     4081 ggttggcaag ccacatttgg cggtggcgac catcctccca agtcagacct agtgcctagg
     4141 cacaaccaga ccagcctata taagaaagcc gggtcagctg ccgctgtgct ggaggagaac
     4201 ctgtatttcc agggcggatc caacatcatg ctcaacaacg atgagctcga atttgaacaa
     4261 gctccagaat tcctgctcgg cgacggctct ttcggctccg tataccgtgc tgcttatgaa
     4321 ggcgaagaag tcgccgtaaa gatcgctaac aagcacacct cgctccgtct gctccgtcag
     4381 gaactggtcg ttctgtgtca tctgcaccat ccgagcctca tctccctgcg tgccgctggc
     4441 atccgtcctc gtatgctggt gatggagctg gcatccaagg gctctctcga ccgcctgctg
     4501 cagcaggaca aggcttctct gacccgtact ctgcagcatc gtatcgctct gcatgtagct
     4561 gacggcctgc gttatcttca ttctgcaatg atcatttatc gcgacctgaa acctcataat
     4621 gtactgctgt ttactctgta cccgaacgct gccatcatcg ctaagattgc agactatggt
     4681 attgcgcagt actgctgccg catgggcatt aaaacaagcg aaggtacgcc gggtttccgt
     4741 gctccagaag tggctcgtgg caacgttatt tacaaccagc aagctgacgt ttactcgttc
     4801 ggtctgctcc tgtacgatat cctgactacc ggcggccgta tcgtagaagg tctgaagttc
     4861 cctaacgaat tcgacgaact ggaaatccag ggcaagctgc cagacccggt taaagaatac
     4921 ggttgcgctc cgtggccgat ggtagagaag ctcatcaaac agtgccttaa agaaaaccct
     4981 caggaacgtc caacctctgc tcaagtgttc gaccagctta acagcgctga actcgtctgt
     5041 ctgactcgcc gtattctcct gcctaagaat gttatcgtgg aatgtatggt tgctactcac
     5101 cataactgat gataagcttt agtaagcact cgagcaccac caccaccacc actaacctag
     5161 gtagctgagc gcatgcaagc tgatccgggt tattagtaca tttattaagc gctagattct
     5221 gtgcgttgtt gatttacaga caattgttgt acgtatttta ataattcatt aaatttataa
     5281 tctttagggt ggtatgttag agcgaaaatc aaatgatttt cagcgtcttt atatctgaat
     5341 ttaaatatta aatcctcaat agatttgtaa aataggtttc gattagtttc aaacaagggt
     5401 tgtttttccg aaccgatggc tggactatct aatggatttt cgctcaacgc cacaaaactt
     5461 gccaaatctt gtagcagcaa tctagctttg tcgatattcg tttgtgtttt gttttgtaat
     5521 aaaggttcga cgtcgttcaa aatattatgc gcttttgtat ttctttcatc actgtcgtta
     5581 gtgtacaatt gactcgacgt aaacacgtta aatagagctt ggacatattt aacatcgggc
     5641 gtgttagctt tattaggccg attatcgtcg tcgtcccaac cctcgtcgtt agaagttgct
     5701 tccgaagacg attttgccat agccacacga cgcctattaa ttgtgtcggc taacacgtcc
     5761 gcgatcaaat ttgtagttga gctttttgga attatttctg attgcgggcg tttttgggcg
     5821 ggtttcaatc taactgtgcc cgattttaat tcagacaaca cgttagaaag cgatggtgca
     5881 ggcggtggta acatttcaga cggcaaatct actaatggcg gcggtggtgg agctgatgat
     5941 aaatctacca tcggtggagg cgcaggcggg gctggcggcg gaggcggagg cggaggtggt
     6001 ggcggtgatg cagacggcgg tttaggctca aatgtctctt taggcaacac agtcggcacc
     6061 tcaactattg tactggtttc gggcgccgtt tttggtttga ccggtctgag acgagtgcga
     6121 tttttttcgt ttctaatagc ttccaacaat tgttgtctgt cgtctaaagg tgcagcgggt
     6181 tgaggttccg tcggcattgg tggagcgggc ggcaattcag acatcgatgg tggtggtggt
     6241 ggtggaggcg ctggaatgtt aggcacggga gaaggtggtg gcggcggtgc cgccggtata
     6301 atttgttctg gtttagtttg ttcgcgcacg attgtgggca ccggcgcagg cgccgctggc
     6361 tgcacaacgg aaggtcgtct gcttcgaggc agcgcttggg gtggtggcaa ttcaatatta
     6421 taattggaat acaaatcgta aaaatctgct ataagcattg taatttcgct atcgtttacc
     6481 gtgccgatat ttaacaaccg ctcaatgtaa gcaattgtat tgtaaagaga ttgtctcaag
     6541 ctcggatcga tcccgcacgc cgataacaag ccttttcatt tttactacag cattgtagtg
     6601 gcgagacact tcgctgtcgt cgaggtttaa acgcttcctc gctcactgac tcgctgcgct
     6661 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     6721 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     6781 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     6841 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     6901 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     6961 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     7021 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     7081 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     7141 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     7201 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     7261 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     7321 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     7381 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     7441 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     7501 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     7561 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     7621 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     7681 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     7741 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     7801 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     7861 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     7921 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     7981 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     8041 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     8101 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     8161 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     8221 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     8281 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     8341 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     8401 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     8461 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     8521 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt agcggcgcat
     8581 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     8641 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     8701 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     8761 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     8821 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     8881 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     8941 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     9001 taacgtttac aatttcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     9061 tgcgggcctc ttcgctatta cgcca
//
