LOCUS       sequence-52172-e        9947 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9947)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB018 / LRRK2_BC_1300_2136
FEATURES             Location/Qualifiers
     source          1..9947
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     329..1156
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             329..453
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(491..1096)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     CDS             complement(1167..2978)
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGQQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFSLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     promoter        complement(2987..3302)
                     /gene="Autographa californica p6.9"
                     /label=p6.9 promoter
                     /note="baculovirus late basic protein promoter"
     promoter        3317..3408
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     3333..3350
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             3421..3444
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             3445..3462
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             5997..6014
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_recomb     6051..7408
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             complement(6067..7408)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     primer_bind     complement(7500..7517)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7671..8259)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7751..7770)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8430..9221,9222..9290))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9053..9072
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9291..9395)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9422..9877
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9509..9528)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9719..9740
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag
       61 agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac
      121 aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat
      181 gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg
      241 gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac
      301 cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat
      361 aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac
      421 tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat
      481 ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa
      541 ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac
      601 agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt
      661 cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt
      721 atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt
      781 ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat
      841 tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt
      901 tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa
      961 ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt
     1021 taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt
     1081 taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta
     1141 tcaatatata gttgctgatc agatcctcat tgtttgcctc cctgctgcgg tttttcaccg
     1201 aagttcatgc cagtccagcg tttttgcagc agaaaagccg ccgacttcgg tttgcggtcg
     1261 cgagtgaaga tccctttctt gttaccgcca acgcgcaata tgccttgcga ggtcgcaaaa
     1321 tcggcgaaat tccatacctg ttcaccgacg acggcgctga cgcgatcaaa gacgcggtga
     1381 tacatatcca gccatgcaca ctgatactct tcactccaca tgtcggtgta cattgagtgc
     1441 agcccggcta acgtatccac gccgtattcg gtgatgataa tcggctgatg cagtttctcc
     1501 tgccaggcca gaagttcttt ttccagtacc ttctctgccg tttccaaatc gccgctttgg
     1561 acataccatc cgtaataacg gttcaggcac agcacatcaa agagatcgct gatggtatcg
     1621 gtgtgagcgt cgcagaacat tacattgacg caggtgatcg gacgcgtcgg gtcgagttta
     1681 cgcgttgctt ccgccagtgg cgcgaaatat tcccgtgcac cttgcggacg ggtatccggt
     1741 tcgttggcaa tactccacat caccacgctt gggtggtttt tgtcacgcgc tatcagctct
     1801 ttaatcgcct gtaagtgcgc ttgctgagtt tccccgttga ctgcctcttc gctgtacagt
     1861 tctttcggct tgttgcccgc ttcgaaacca atgcctaaag agagcgaaaa gccgacagca
     1921 gcagtttcat caatcaccac gatgccatgt tcatctgccc agtcgagcat ctcttcagcg
     1981 taagggtaat gcgaggtacg gtaggagttg gccccaatcc agtccattaa tgcgtggtcg
     2041 tgcaccatca gcacgttatc gaatcctttg ccacgtaagt ccgcatcttc atgacgacca
     2101 aagccagtaa agtagaacgg tttgtggtta atcaggaact gttggccctt cactgccact
     2161 gaccggatgc cgacgcgaag cgggtagata tcacactctg tctggctttt ggctgtgacg
     2221 cacagttcat agagataacc ttcacccggt tgccagaggt gcggattcac cacttgcaaa
     2281 gtcccgctag tgccttgtcc agttgcaacc acctgttgat ccgcatcacg cagttcaacg
     2341 ctgacatcac cattggccac cacctgccag tcaacagacg cgtggttaca gtcttgcgcg
     2401 acatgcgtca ccacggtgat atcgtccacc caggtgttcg gcgtggtgta gagcattacg
     2461 ctgcgatgga ttccggcata gttaaagaaa tcatggaagt aagactgctt tttcttgccg
     2521 ttttcgtcgg taatcaccat tcccggcggg atagtctgcc agttcagttc gttgttcaca
     2581 caaacggtga tacgtacact tttcccggca ataacatacg gcgtgacatc ggcttcaaat
     2641 ggcgtatagc cgccctgatg ctccatcact tcctgattat tgacccacac tttgccgtaa
     2701 tgagtgaccg catcgaaacg cagcacgata cgctggcctg cccaaccttt cggtataaag
     2761 acttcgcgct gataccagac gttgcccgca taattacgaa tatctgcatc ggcgaactga
     2821 tcgttaaaac tgcctggcac agcaattgcc cggctttctt gtaacgcgct ttcccaccaa
     2881 cgctgatcaa ttccacagtt ttcgcgatcc agactgaatg cccacaggcc gtcgagtttt
     2941 ttgatttcac gggttggggt ttctacagga cggaccatat ttataggtgt aatttatgta
     3001 gctgtaattt ttaccttatt aatatttttt acgctttgca ttcgacgact gaactcccaa
     3061 atatatgttt aactcgtctt ggtcgtttga atttttgttg ctgtgtttcc taatattttc
     3121 catcacctta aatatgttat tgtaatcctc aatgttgaac ttgcaattgg acacggcata
     3181 gttttccata gtcgtgtaaa acatggtatt ggctgcattg taatacatcc gactgagcgg
     3241 gtacggatct atgtgtttga gcagcctgtt caaaaactct gcatcgtcgc aaaacggaat
     3301 ttcgtcgaca gatctgatca tggagataat taaaatgata accatctcgc aaataaataa
     3361 gtattttact gttttcgtaa cagttttgta ataaaaaaac ctataaatat aggatctatg
     3421 caccaccatc accaccatca ccacttggtg ccccgcggat ccgacgagct gcacctaaac
     3481 ttcgacttca agcacattgg ctgtaaagca aaggatatta ttcgtttcct gcagcagcgt
     3541 cttaagaagg ccgtgcccta caatcgcatg aagctgatga tcgtcgggaa caccggttct
     3601 ggaaagacaa ccctcttgca gcagcttatg aagactaaga agtccgactt ggggatgcag
     3661 agcgccactg tggggatcga cgtgaaagac tggccaatcc aaatccgtga caagcgcaag
     3721 cgtgacttag tactgaacgt gtgggacttt gccggcaggg aagagttcta ctctacgcat
     3781 cctcacttca tgactcagcg tgccctctat ctggcagttt acgacctctc aaagggccag
     3841 gctgaggtgg acgccatgaa accctggctg tttaacatca aggctcgtgc ttccagctcc
     3901 cctgtgatct tagtcgggac acacttggat gtttctgatg agaagcagcg taaggcatgt
     3961 atgagtaaga tcaccaaaga gctgttgaac aaacgcggct ttcccgccat ccgtgattac
     4021 cacttcgtca acgctaccga ggaaagcgac gcactggcca agttacgtaa gaccattatc
     4081 aacgaaagcc tgaacttcaa aattcgcgac cagctggtgg tcggacagct gatccccgac
     4141 tgctacgtgg aacttgagaa gatcatcctg agcgagcgca agaatgtgcc tatcgagttt
     4201 ccagtgatcg atcgcaaacg tctcctccag ctcgtgcgtg agaatcagct gcagctcgac
     4261 gagaacgagc tgcctcatgc tgtgcacttc cttaacgagt caggtgtcct cctgcacttc
     4321 caggacccgg ctctccagct gagtgacctc tatttcgtgg agccaaagtg gctctgtaaa
     4381 attatggctc aaatcctcac tgtcaaggta gagggctgtc caaagcaccc aaagggcatc
     4441 atctcgcgtc gcgacgtgga aaagtttctc tctaagaagc gcaagtttcc caagaactac
     4501 atgagccagt actttaaact gttggagaag ttccagatcg ctctgcctat cggcgaagag
     4561 tacctgttgg ttccttcctc cctgtccgat caccgccccg tgattgagct gcctcactgc
     4621 gagaacagcg agattatcat tcgcctatac gagatgccgt actttccgat gggcttctgg
     4681 tcaaggctca tcaatcgtct gcttgaaatc tccccatata tgctgagcgg tcgtgaacgc
     4741 gctctgaggc caaaccggat gtactggcgc caggggattt acttgaactg gtcccctgag
     4801 gcttattgtc tggtgggctc ggaagtcctg gataaccacc ccgaatcatt cctgaagatc
     4861 accgttcctt cctgccgtaa gggttgcatt ctattgggac aggtggtgga tcatatcgat
     4921 tccctgatgg aggagtggtt ccctggcctg ttggagatcg acatctgtgg cgagggtgag
     4981 accctgctga agaagtgggc actctacagt ttcaatgacg gcgaagaaca ccagaagatt
     5041 ctgctcgacg acctcatgaa gaaggccgag gagggcgacc tgctcgtgaa ccccgaccag
     5101 cctaggctga ccatccccat ctcccagatt gcacccgacc ttatcctcgc tgacctgcct
     5161 cgcaacatca tgctgaacaa tgacgagctg gagtttgaac aagcacccga gttcctgcta
     5221 ggcgacggct ctttcggttc agtttaccgt gctgcttacg agggtgaaga ggttgccgtg
     5281 aagattttta acaagcacac ttcgctccgt ctgctgcgtc aggagcttgt ggttctctgc
     5341 cacctgcacc acccttccct gatctccctg ctggccgccg gcattaggcc tcgcatgcta
     5401 gtgatggaac tggccagtaa gggttcattg gaccgcctgc tgcagcagga caaggctagc
     5461 ctgacccgta cgctccagca ccgtatcgcc ctgcatgtgg ccgacggact gcgctacttg
     5521 cacagtgcca tgatcattta tcgcgatctg aagcctcata atgttcttct gttcacgctc
     5581 tacccaaacg cagctatcat tgccaaaatt gccgattacg gcatagccca gtactgctgt
     5641 cgtatgggta tcaagactag cgagggtaca cctgggttcc gcgctcccga agtcgcacgc
     5701 gggaacgtga tctacaacca gcaggccgac gtgtactcgt ttggcctgct cctgtatgac
     5761 attctcacta ccggaggccg gattgtggag ggcctgaaat ttcccaacga gttcgacgag
     5821 ctggagatcc agggcaagct gcctgacccc gtgaaggagt acggttgtgc accctggcct
     5881 atggtggaga agctcattaa gcagtgtctg aaggagaacc ctcaggaacg ccccacaagc
     5941 gcccaggtgt tcgacattct aaactctgca gagtaaagct tgcggccgca ctcgagcacc
     6001 accaccacca ccactaacct aggtagctga gcgcatgcaa gctgatccgg gttattagta
     6061 catttattaa gcgctagatt ctgtgcgttg ttgatttaca gacaattgtt gtacgtattt
     6121 taataattca ttaaatttat aatctttagg gtggtatgtt agagcgaaaa tcaaatgatt
     6181 ttcagcgtct ttatatctga atttaaatat taaatcctca atagatttgt aaaataggtt
     6241 tcgattagtt tcaaacaagg gttgtttttc cgaaccgatg gctggactat ctaatggatt
     6301 ttcgctcaac gccacaaaac ttgccaaatc ttgtagcagc aatctagctt tgtcgatatt
     6361 cgtttgtgtt ttgttttgta ataaaggttc gacgtcgttc aaaatattat gcgcttttgt
     6421 atttctttca tcactgtcgt tagtgtacaa ttgactcgac gtaaacacgt taaatagagc
     6481 ttggacatat ttaacatcgg gcgtgttagc tttattaggc cgattatcgt cgtcgtccca
     6541 accctcgtcg ttagaagttg cttccgaaga cgattttgcc atagccacac gacgcctatt
     6601 aattgtgtcg gctaacacgt ccgcgatcaa atttgtagtt gagctttttg gaattatttc
     6661 tgattgcggg cgtttttggg cgggtttcaa tctaactgtg cccgatttta attcagacaa
     6721 cacgttagaa agcgatggtg caggcggtgg taacatttca gacggcaaat ctactaatgg
     6781 cggcggtggt ggagctgatg ataaatctac catcggtgga ggcgcaggcg gggctggcgg
     6841 cggaggcgga ggcggaggtg gtggcggtga tgcagacggc ggtttaggct caaatgtctc
     6901 tttaggcaac acagtcggca cctcaactat tgtactggtt tcgggcgccg tttttggttt
     6961 gaccggtctg agacgagtgc gatttttttc gtttctaata gcttccaaca attgttgtct
     7021 gtcgtctaaa ggtgcagcgg gttgaggttc cgtcggcatt ggtggagcgg gcggcaattc
     7081 agacatcgat ggtggtggtg gtggtggagg cgctggaatg ttaggcacgg gagaaggtgg
     7141 tggcggcggt gccgccggta taatttgttc tggtttagtt tgttcgcgca cgattgtggg
     7201 caccggcgca ggcgccgctg gctgcacaac ggaaggtcgt ctgcttcgag gcagcgcttg
     7261 gggtggtggc aattcaatat tataattgga atacaaatcg taaaaatctg ctataagcat
     7321 tgtaatttcg ctatcgttta ccgtgccgat atttaacaac cgctcaatgt aagcaattgt
     7381 attgtaaaga gattgtctca agctcggatc gatcccgcac gccgataaca agccttttca
     7441 tttttactac agcattgtag tggcgagaca cttcgctgtc gtcgaggttt aaacgcttcc
     7501 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     7561 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     7621 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     7681 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     7741 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     7801 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     7861 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     7921 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     7981 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     8041 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     8101 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     8161 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     8221 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     8281 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     8341 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     8401 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     8461 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     8521 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     8581 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     8641 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     8701 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     8761 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     8821 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     8881 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     8941 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     9001 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     9061 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     9121 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     9181 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     9241 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     9301 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     9361 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     9421 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     9481 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     9541 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     9601 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     9661 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     9721 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     9781 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     9841 aacgcgaatt ttaacaaaat attaacgttt acaatttccc attcgccatt caggctgcgc
     9901 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgcca
//
