LOCUS       sequence-52176-e        9920 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9920)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB018 / LRRK2_BC_1309_2136
FEATURES             Location/Qualifiers
     source          1..9920
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     329..1156
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             329..453
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(491..1096)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     CDS             complement(1167..2978)
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGQQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFSLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     promoter        complement(2987..3302)
                     /gene="Autographa californica p6.9"
                     /label=p6.9 promoter
                     /note="baculovirus late basic protein promoter"
     promoter        3317..3408
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     3333..3350
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             3421..3444
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             3445..3462
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             5970..5987
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_recomb     6024..7381
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             complement(6040..7381)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     primer_bind     complement(7473..7490)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7644..8232)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7724..7743)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8403..9194,9195..9263))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9026..9045
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9264..9368)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9395..9850
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9482..9501)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9692..9713
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag
       61 agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac
      121 aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat
      181 gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg
      241 gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac
      301 cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat
      361 aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac
      421 tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat
      481 ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa
      541 ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac
      601 agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt
      661 cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt
      721 atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt
      781 ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat
      841 tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt
      901 tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa
      961 ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt
     1021 taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt
     1081 taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta
     1141 tcaatatata gttgctgatc agatcctcat tgtttgcctc cctgctgcgg tttttcaccg
     1201 aagttcatgc cagtccagcg tttttgcagc agaaaagccg ccgacttcgg tttgcggtcg
     1261 cgagtgaaga tccctttctt gttaccgcca acgcgcaata tgccttgcga ggtcgcaaaa
     1321 tcggcgaaat tccatacctg ttcaccgacg acggcgctga cgcgatcaaa gacgcggtga
     1381 tacatatcca gccatgcaca ctgatactct tcactccaca tgtcggtgta cattgagtgc
     1441 agcccggcta acgtatccac gccgtattcg gtgatgataa tcggctgatg cagtttctcc
     1501 tgccaggcca gaagttcttt ttccagtacc ttctctgccg tttccaaatc gccgctttgg
     1561 acataccatc cgtaataacg gttcaggcac agcacatcaa agagatcgct gatggtatcg
     1621 gtgtgagcgt cgcagaacat tacattgacg caggtgatcg gacgcgtcgg gtcgagttta
     1681 cgcgttgctt ccgccagtgg cgcgaaatat tcccgtgcac cttgcggacg ggtatccggt
     1741 tcgttggcaa tactccacat caccacgctt gggtggtttt tgtcacgcgc tatcagctct
     1801 ttaatcgcct gtaagtgcgc ttgctgagtt tccccgttga ctgcctcttc gctgtacagt
     1861 tctttcggct tgttgcccgc ttcgaaacca atgcctaaag agagcgaaaa gccgacagca
     1921 gcagtttcat caatcaccac gatgccatgt tcatctgccc agtcgagcat ctcttcagcg
     1981 taagggtaat gcgaggtacg gtaggagttg gccccaatcc agtccattaa tgcgtggtcg
     2041 tgcaccatca gcacgttatc gaatcctttg ccacgtaagt ccgcatcttc atgacgacca
     2101 aagccagtaa agtagaacgg tttgtggtta atcaggaact gttggccctt cactgccact
     2161 gaccggatgc cgacgcgaag cgggtagata tcacactctg tctggctttt ggctgtgacg
     2221 cacagttcat agagataacc ttcacccggt tgccagaggt gcggattcac cacttgcaaa
     2281 gtcccgctag tgccttgtcc agttgcaacc acctgttgat ccgcatcacg cagttcaacg
     2341 ctgacatcac cattggccac cacctgccag tcaacagacg cgtggttaca gtcttgcgcg
     2401 acatgcgtca ccacggtgat atcgtccacc caggtgttcg gcgtggtgta gagcattacg
     2461 ctgcgatgga ttccggcata gttaaagaaa tcatggaagt aagactgctt tttcttgccg
     2521 ttttcgtcgg taatcaccat tcccggcggg atagtctgcc agttcagttc gttgttcaca
     2581 caaacggtga tacgtacact tttcccggca ataacatacg gcgtgacatc ggcttcaaat
     2641 ggcgtatagc cgccctgatg ctccatcact tcctgattat tgacccacac tttgccgtaa
     2701 tgagtgaccg catcgaaacg cagcacgata cgctggcctg cccaaccttt cggtataaag
     2761 acttcgcgct gataccagac gttgcccgca taattacgaa tatctgcatc ggcgaactga
     2821 tcgttaaaac tgcctggcac agcaattgcc cggctttctt gtaacgcgct ttcccaccaa
     2881 cgctgatcaa ttccacagtt ttcgcgatcc agactgaatg cccacaggcc gtcgagtttt
     2941 ttgatttcac gggttggggt ttctacagga cggaccatat ttataggtgt aatttatgta
     3001 gctgtaattt ttaccttatt aatatttttt acgctttgca ttcgacgact gaactcccaa
     3061 atatatgttt aactcgtctt ggtcgtttga atttttgttg ctgtgtttcc taatattttc
     3121 catcacctta aatatgttat tgtaatcctc aatgttgaac ttgcaattgg acacggcata
     3181 gttttccata gtcgtgtaaa acatggtatt ggctgcattg taatacatcc gactgagcgg
     3241 gtacggatct atgtgtttga gcagcctgtt caaaaactct gcatcgtcgc aaaacggaat
     3301 ttcgtcgaca gatctgatca tggagataat taaaatgata accatctcgc aaataaataa
     3361 gtattttact gttttcgtaa cagttttgta ataaaaaaac ctataaatat aggatctatg
     3421 caccaccatc accaccatca ccacttggtg ccccgcggat ccaagcacat tggctgtaaa
     3481 gcaaaggata ttattcgttt cctgcagcag cgtcttaaga aggccgtgcc ctacaatcgc
     3541 atgaagctga tgatcgtcgg gaacaccggt tctggaaaga caaccctctt gcagcagctt
     3601 atgaagacta agaagtccga cttggggatg cagagcgcca ctgtggggat cgacgtgaaa
     3661 gactggccaa tccaaatccg tgacaagcgc aagcgtgact tagtactgaa cgtgtgggac
     3721 tttgccggca gggaagagtt ctactctacg catcctcact tcatgactca gcgtgccctc
     3781 tatctggcag tttacgacct ctcaaagggc caggctgagg tggacgccat gaaaccctgg
     3841 ctgtttaaca tcaaggctcg tgcttccagc tcccctgtga tcttagtcgg gacacacttg
     3901 gatgtttctg atgagaagca gcgtaaggca tgtatgagta agatcaccaa agagctgttg
     3961 aacaaacgcg gctttcccgc catccgtgat taccacttcg tcaacgctac cgaggaaagc
     4021 gacgcactgg ccaagttacg taagaccatt atcaacgaaa gcctgaactt caaaattcgc
     4081 gaccagctgg tggtcggaca gctgatcccc gactgctacg tggaacttga gaagatcatc
     4141 ctgagcgagc gcaagaatgt gcctatcgag tttccagtga tcgatcgcaa acgtctcctc
     4201 cagctcgtgc gtgagaatca gctgcagctc gacgagaacg agctgcctca tgctgtgcac
     4261 ttccttaacg agtcaggtgt cctcctgcac ttccaggacc cggctctcca gctgagtgac
     4321 ctctatttcg tggagccaaa gtggctctgt aaaattatgg ctcaaatcct cactgtcaag
     4381 gtagagggct gtccaaagca cccaaagggc atcatctcgc gtcgcgacgt ggaaaagttt
     4441 ctctctaaga agcgcaagtt tcccaagaac tacatgagcc agtactttaa actgttggag
     4501 aagttccaga tcgctctgcc tatcggcgaa gagtacctgt tggttccttc ctccctgtcc
     4561 gatcaccgcc ccgtgattga gctgcctcac tgcgagaaca gcgagattat cattcgccta
     4621 tacgagatgc cgtactttcc gatgggcttc tggtcaaggc tcatcaatcg tctgcttgaa
     4681 atctccccat atatgctgag cggtcgtgaa cgcgctctga ggccaaaccg gatgtactgg
     4741 cgccagggga tttacttgaa ctggtcccct gaggcttatt gtctggtggg ctcggaagtc
     4801 ctggataacc accccgaatc attcctgaag atcaccgttc cttcctgccg taagggttgc
     4861 attctattgg gacaggtggt ggatcatatc gattccctga tggaggagtg gttccctggc
     4921 ctgttggaga tcgacatctg tggcgagggt gagaccctgc tgaagaagtg ggcactctac
     4981 agtttcaatg acggcgaaga acaccagaag attctgctcg acgacctcat gaagaaggcc
     5041 gaggagggcg acctgctcgt gaaccccgac cagcctaggc tgaccatccc catctcccag
     5101 attgcacccg accttatcct cgctgacctg cctcgcaaca tcatgctgaa caatgacgag
     5161 ctggagtttg aacaagcacc cgagttcctg ctaggcgacg gctctttcgg ttcagtttac
     5221 cgtgctgctt acgagggtga agaggttgcc gtgaagattt ttaacaagca cacttcgctc
     5281 cgtctgctgc gtcaggagct tgtggttctc tgccacctgc accacccttc cctgatctcc
     5341 ctgctggccg ccggcattag gcctcgcatg ctagtgatgg aactggccag taagggttca
     5401 ttggaccgcc tgctgcagca ggacaaggct agcctgaccc gtacgctcca gcaccgtatc
     5461 gccctgcatg tggccgacgg actgcgctac ttgcacagtg ccatgatcat ttatcgcgat
     5521 ctgaagcctc ataatgttct tctgttcacg ctctacccaa acgcagctat cattgccaaa
     5581 attgccgatt acggcatagc ccagtactgc tgtcgtatgg gtatcaagac tagcgagggt
     5641 acacctgggt tccgcgctcc cgaagtcgca cgcgggaacg tgatctacaa ccagcaggcc
     5701 gacgtgtact cgtttggcct gctcctgtat gacattctca ctaccggagg ccggattgtg
     5761 gagggcctga aatttcccaa cgagttcgac gagctggaga tccagggcaa gctgcctgac
     5821 cccgtgaagg agtacggttg tgcaccctgg cctatggtgg agaagctcat taagcagtgt
     5881 ctgaaggaga accctcagga acgccccaca agcgcccagg tgttcgacat tctaaactct
     5941 gcagagtaaa gcttgcggcc gcactcgagc accaccacca ccaccactaa cctaggtagc
     6001 tgagcgcatg caagctgatc cgggttatta gtacatttat taagcgctag attctgtgcg
     6061 ttgttgattt acagacaatt gttgtacgta ttttaataat tcattaaatt tataatcttt
     6121 agggtggtat gttagagcga aaatcaaatg attttcagcg tctttatatc tgaatttaaa
     6181 tattaaatcc tcaatagatt tgtaaaatag gtttcgatta gtttcaaaca agggttgttt
     6241 ttccgaaccg atggctggac tatctaatgg attttcgctc aacgccacaa aacttgccaa
     6301 atcttgtagc agcaatctag ctttgtcgat attcgtttgt gttttgtttt gtaataaagg
     6361 ttcgacgtcg ttcaaaatat tatgcgcttt tgtatttctt tcatcactgt cgttagtgta
     6421 caattgactc gacgtaaaca cgttaaatag agcttggaca tatttaacat cgggcgtgtt
     6481 agctttatta ggccgattat cgtcgtcgtc ccaaccctcg tcgttagaag ttgcttccga
     6541 agacgatttt gccatagcca cacgacgcct attaattgtg tcggctaaca cgtccgcgat
     6601 caaatttgta gttgagcttt ttggaattat ttctgattgc gggcgttttt gggcgggttt
     6661 caatctaact gtgcccgatt ttaattcaga caacacgtta gaaagcgatg gtgcaggcgg
     6721 tggtaacatt tcagacggca aatctactaa tggcggcggt ggtggagctg atgataaatc
     6781 taccatcggt ggaggcgcag gcggggctgg cggcggaggc ggaggcggag gtggtggcgg
     6841 tgatgcagac ggcggtttag gctcaaatgt ctctttaggc aacacagtcg gcacctcaac
     6901 tattgtactg gtttcgggcg ccgtttttgg tttgaccggt ctgagacgag tgcgattttt
     6961 ttcgtttcta atagcttcca acaattgttg tctgtcgtct aaaggtgcag cgggttgagg
     7021 ttccgtcggc attggtggag cgggcggcaa ttcagacatc gatggtggtg gtggtggtgg
     7081 aggcgctgga atgttaggca cgggagaagg tggtggcggc ggtgccgccg gtataatttg
     7141 ttctggttta gtttgttcgc gcacgattgt gggcaccggc gcaggcgccg ctggctgcac
     7201 aacggaaggt cgtctgcttc gaggcagcgc ttggggtggt ggcaattcaa tattataatt
     7261 ggaatacaaa tcgtaaaaat ctgctataag cattgtaatt tcgctatcgt ttaccgtgcc
     7321 gatatttaac aaccgctcaa tgtaagcaat tgtattgtaa agagattgtc tcaagctcgg
     7381 atcgatcccg cacgccgata acaagccttt tcatttttac tacagcattg tagtggcgag
     7441 acacttcgct gtcgtcgagg tttaaacgct tcctcgctca ctgactcgct gcgctcggtc
     7501 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     7561 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     7621 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     7681 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     7741 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     7801 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     7861 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     7921 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     7981 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     8041 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     8101 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     8161 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     8221 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     8281 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     8341 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     8401 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     8461 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     8521 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     8581 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     8641 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     8701 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     8761 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     8821 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     8881 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     8941 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     9001 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     9061 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     9121 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     9181 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     9241 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     9301 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     9361 gttccgcgca catttccccg aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc
     9421 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     9481 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     9541 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     9601 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     9661 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     9721 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     9781 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     9841 tttacaattt cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     9901 gcctcttcgc tattacgcca
//
