LOCUS       sequence-52178-e       10073 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10073)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB018 / LRRK2_BC_1309_2187
FEATURES             Location/Qualifiers
     source          1..10073
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     329..1156
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             329..453
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(491..1096)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     CDS             complement(1167..2978)
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGQQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFSLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     promoter        complement(2987..3302)
                     /gene="Autographa californica p6.9"
                     /label=p6.9 promoter
                     /note="baculovirus late basic protein promoter"
     promoter        3317..3408
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     3333..3350
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             3421..3444
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             3445..3462
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             6123..6140
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_recomb     6177..7534
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             complement(6193..7534)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     primer_bind     complement(7626..7643)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7797..8385)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7877..7896)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8556..9347,9348..9416))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9179..9198
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9417..9521)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9548..10003
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9635..9654)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9845..9866
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag
       61 agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac
      121 aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat
      181 gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg
      241 gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac
      301 cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat
      361 aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac
      421 tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat
      481 ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa
      541 ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac
      601 agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt
      661 cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt
      721 atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt
      781 ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat
      841 tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt
      901 tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa
      961 ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt
     1021 taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt
     1081 taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta
     1141 tcaatatata gttgctgatc agatcctcat tgtttgcctc cctgctgcgg tttttcaccg
     1201 aagttcatgc cagtccagcg tttttgcagc agaaaagccg ccgacttcgg tttgcggtcg
     1261 cgagtgaaga tccctttctt gttaccgcca acgcgcaata tgccttgcga ggtcgcaaaa
     1321 tcggcgaaat tccatacctg ttcaccgacg acggcgctga cgcgatcaaa gacgcggtga
     1381 tacatatcca gccatgcaca ctgatactct tcactccaca tgtcggtgta cattgagtgc
     1441 agcccggcta acgtatccac gccgtattcg gtgatgataa tcggctgatg cagtttctcc
     1501 tgccaggcca gaagttcttt ttccagtacc ttctctgccg tttccaaatc gccgctttgg
     1561 acataccatc cgtaataacg gttcaggcac agcacatcaa agagatcgct gatggtatcg
     1621 gtgtgagcgt cgcagaacat tacattgacg caggtgatcg gacgcgtcgg gtcgagttta
     1681 cgcgttgctt ccgccagtgg cgcgaaatat tcccgtgcac cttgcggacg ggtatccggt
     1741 tcgttggcaa tactccacat caccacgctt gggtggtttt tgtcacgcgc tatcagctct
     1801 ttaatcgcct gtaagtgcgc ttgctgagtt tccccgttga ctgcctcttc gctgtacagt
     1861 tctttcggct tgttgcccgc ttcgaaacca atgcctaaag agagcgaaaa gccgacagca
     1921 gcagtttcat caatcaccac gatgccatgt tcatctgccc agtcgagcat ctcttcagcg
     1981 taagggtaat gcgaggtacg gtaggagttg gccccaatcc agtccattaa tgcgtggtcg
     2041 tgcaccatca gcacgttatc gaatcctttg ccacgtaagt ccgcatcttc atgacgacca
     2101 aagccagtaa agtagaacgg tttgtggtta atcaggaact gttggccctt cactgccact
     2161 gaccggatgc cgacgcgaag cgggtagata tcacactctg tctggctttt ggctgtgacg
     2221 cacagttcat agagataacc ttcacccggt tgccagaggt gcggattcac cacttgcaaa
     2281 gtcccgctag tgccttgtcc agttgcaacc acctgttgat ccgcatcacg cagttcaacg
     2341 ctgacatcac cattggccac cacctgccag tcaacagacg cgtggttaca gtcttgcgcg
     2401 acatgcgtca ccacggtgat atcgtccacc caggtgttcg gcgtggtgta gagcattacg
     2461 ctgcgatgga ttccggcata gttaaagaaa tcatggaagt aagactgctt tttcttgccg
     2521 ttttcgtcgg taatcaccat tcccggcggg atagtctgcc agttcagttc gttgttcaca
     2581 caaacggtga tacgtacact tttcccggca ataacatacg gcgtgacatc ggcttcaaat
     2641 ggcgtatagc cgccctgatg ctccatcact tcctgattat tgacccacac tttgccgtaa
     2701 tgagtgaccg catcgaaacg cagcacgata cgctggcctg cccaaccttt cggtataaag
     2761 acttcgcgct gataccagac gttgcccgca taattacgaa tatctgcatc ggcgaactga
     2821 tcgttaaaac tgcctggcac agcaattgcc cggctttctt gtaacgcgct ttcccaccaa
     2881 cgctgatcaa ttccacagtt ttcgcgatcc agactgaatg cccacaggcc gtcgagtttt
     2941 ttgatttcac gggttggggt ttctacagga cggaccatat ttataggtgt aatttatgta
     3001 gctgtaattt ttaccttatt aatatttttt acgctttgca ttcgacgact gaactcccaa
     3061 atatatgttt aactcgtctt ggtcgtttga atttttgttg ctgtgtttcc taatattttc
     3121 catcacctta aatatgttat tgtaatcctc aatgttgaac ttgcaattgg acacggcata
     3181 gttttccata gtcgtgtaaa acatggtatt ggctgcattg taatacatcc gactgagcgg
     3241 gtacggatct atgtgtttga gcagcctgtt caaaaactct gcatcgtcgc aaaacggaat
     3301 ttcgtcgaca gatctgatca tggagataat taaaatgata accatctcgc aaataaataa
     3361 gtattttact gttttcgtaa cagttttgta ataaaaaaac ctataaatat aggatctatg
     3421 caccaccatc accaccatca ccacttggtg ccccgcggat ccaagcacat tggctgtaaa
     3481 gcaaaggata ttattcgttt cctgcagcag cgtcttaaga aggccgtgcc ctacaatcgc
     3541 atgaagctga tgatcgtcgg gaacaccggt tctggaaaga caaccctctt gcagcagctt
     3601 atgaagacta agaagtccga cttggggatg cagagcgcca ctgtggggat cgacgtgaaa
     3661 gactggccaa tccaaatccg tgacaagcgc aagcgtgact tagtactgaa cgtgtgggac
     3721 tttgccggca gggaagagtt ctactctacg catcctcact tcatgactca gcgtgccctc
     3781 tatctggcag tttacgacct ctcaaagggc caggctgagg tggacgccat gaaaccctgg
     3841 ctgtttaaca tcaaggctcg tgcttccagc tcccctgtga tcttagtcgg gacacacttg
     3901 gatgtttctg atgagaagca gcgtaaggca tgtatgagta agatcaccaa agagctgttg
     3961 aacaaacgcg gctttcccgc catccgtgat taccacttcg tcaacgctac cgaggaaagc
     4021 gacgcactgg ccaagttacg taagaccatt atcaacgaaa gcctgaactt caaaattcgc
     4081 gaccagctgg tggtcggaca gctgatcccc gactgctacg tggaacttga gaagatcatc
     4141 ctgagcgagc gcaagaatgt gcctatcgag tttccagtga tcgatcgcaa acgtctcctc
     4201 cagctcgtgc gtgagaatca gctgcagctc gacgagaacg agctgcctca tgctgtgcac
     4261 ttccttaacg agtcaggtgt cctcctgcac ttccaggacc cggctctcca gctgagtgac
     4321 ctctatttcg tggagccaaa gtggctctgt aaaattatgg ctcaaatcct cactgtcaag
     4381 gtagagggct gtccaaagca cccaaagggc atcatctcgc gtcgcgacgt ggaaaagttt
     4441 ctctctaaga agcgcaagtt tcccaagaac tacatgagcc agtactttaa actgttggag
     4501 aagttccaga tcgctctgcc tatcggcgaa gagtacctgt tggttccttc ctccctgtcc
     4561 gatcaccgcc ccgtgattga gctgcctcac tgcgagaaca gcgagattat cattcgccta
     4621 tacgagatgc cgtactttcc gatgggcttc tggtcaaggc tcatcaatcg tctgcttgaa
     4681 atctccccat atatgctgag cggtcgtgaa cgcgctctga ggccaaaccg gatgtactgg
     4741 cgccagggga tttacttgaa ctggtcccct gaggcttatt gtctggtggg ctcggaagtc
     4801 ctggataacc accccgaatc attcctgaag atcaccgttc cttcctgccg taagggttgc
     4861 attctattgg gacaggtggt ggatcatatc gattccctga tggaggagtg gttccctggc
     4921 ctgttggaga tcgacatctg tggcgagggt gagaccctgc tgaagaagtg ggcactctac
     4981 agtttcaatg acggcgaaga acaccagaag attctgctcg acgacctcat gaagaaggcc
     5041 gaggagggcg acctgctcgt gaaccccgac cagcctaggc tgaccatccc catctcccag
     5101 attgcacccg accttatcct cgctgacctg cctcgcaaca tcatgctgaa caatgacgag
     5161 ctggagtttg aacaagcacc cgagttcctg ctaggcgacg gctctttcgg ttcagtttac
     5221 cgtgctgctt acgagggtga agaggttgcc gtgaagattt ttaacaagca cacttcgctc
     5281 cgtctgctgc gtcaggagct tgtggttctc tgccacctgc accacccttc cctgatctcc
     5341 ctgctggccg ccggcattag gcctcgcatg ctagtgatgg aactggccag taagggttca
     5401 ttggaccgcc tgctgcagca ggacaaggct agcctgaccc gtacgctcca gcaccgtatc
     5461 gccctgcatg tggccgacgg actgcgctac ttgcacagtg ccatgatcat ttatcgcgat
     5521 ctgaagcctc ataatgttct tctgttcacg ctctacccaa acgcagctat cattgccaaa
     5581 attgccgatt acggcatagc ccagtactgc tgtcgtatgg gtatcaagac tagcgagggt
     5641 acacctgggt tccgcgctcc cgaagtcgca cgcgggaacg tgatctacaa ccagcaggcc
     5701 gacgtgtact cgtttggcct gctcctgtat gacattctca ctaccggagg ccggattgtg
     5761 gagggcctga aatttcccaa cgagttcgac gagctggaga tccagggcaa gctgcctgac
     5821 cccgtgaagg agtacggttg tgcaccctgg cctatggtgg agaagctcat taagcagtgt
     5881 ctgaaggaga accctcagga acgccccaca agcgcccagg tgttcgacat tctaaactct
     5941 gcagagttag tgtgtctgac taggcgtatc ttactcccga agaacgtgat cgttgagtgc
     6001 atggtggcaa cgcaccacaa ctcgcgtaac gctagcatct ggttaggatg tgggcatacc
     6061 gatcgtggcc agctgagttt cctcgacctc aacaccgagt aaagcttgcg gccgcactcg
     6121 agcaccacca ccaccaccac taacctaggt agctgagcgc atgcaagctg atccgggtta
     6181 ttagtacatt tattaagcgc tagattctgt gcgttgttga tttacagaca attgttgtac
     6241 gtattttaat aattcattaa atttataatc tttagggtgg tatgttagag cgaaaatcaa
     6301 atgattttca gcgtctttat atctgaattt aaatattaaa tcctcaatag atttgtaaaa
     6361 taggtttcga ttagtttcaa acaagggttg tttttccgaa ccgatggctg gactatctaa
     6421 tggattttcg ctcaacgcca caaaacttgc caaatcttgt agcagcaatc tagctttgtc
     6481 gatattcgtt tgtgttttgt tttgtaataa aggttcgacg tcgttcaaaa tattatgcgc
     6541 ttttgtattt ctttcatcac tgtcgttagt gtacaattga ctcgacgtaa acacgttaaa
     6601 tagagcttgg acatatttaa catcgggcgt gttagcttta ttaggccgat tatcgtcgtc
     6661 gtcccaaccc tcgtcgttag aagttgcttc cgaagacgat tttgccatag ccacacgacg
     6721 cctattaatt gtgtcggcta acacgtccgc gatcaaattt gtagttgagc tttttggaat
     6781 tatttctgat tgcgggcgtt tttgggcggg tttcaatcta actgtgcccg attttaattc
     6841 agacaacacg ttagaaagcg atggtgcagg cggtggtaac atttcagacg gcaaatctac
     6901 taatggcggc ggtggtggag ctgatgataa atctaccatc ggtggaggcg caggcggggc
     6961 tggcggcgga ggcggaggcg gaggtggtgg cggtgatgca gacggcggtt taggctcaaa
     7021 tgtctcttta ggcaacacag tcggcacctc aactattgta ctggtttcgg gcgccgtttt
     7081 tggtttgacc ggtctgagac gagtgcgatt tttttcgttt ctaatagctt ccaacaattg
     7141 ttgtctgtcg tctaaaggtg cagcgggttg aggttccgtc ggcattggtg gagcgggcgg
     7201 caattcagac atcgatggtg gtggtggtgg tggaggcgct ggaatgttag gcacgggaga
     7261 aggtggtggc ggcggtgccg ccggtataat ttgttctggt ttagtttgtt cgcgcacgat
     7321 tgtgggcacc ggcgcaggcg ccgctggctg cacaacggaa ggtcgtctgc ttcgaggcag
     7381 cgcttggggt ggtggcaatt caatattata attggaatac aaatcgtaaa aatctgctat
     7441 aagcattgta atttcgctat cgtttaccgt gccgatattt aacaaccgct caatgtaagc
     7501 aattgtattg taaagagatt gtctcaagct cggatcgatc ccgcacgccg ataacaagcc
     7561 ttttcatttt tactacagca ttgtagtggc gagacacttc gctgtcgtcg aggtttaaac
     7621 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     7681 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     7741 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     7801 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     7861 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     7921 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     7981 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     8041 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     8101 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     8161 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     8221 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     8281 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     8341 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     8401 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     8461 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     8521 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     8581 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     8641 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     8701 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     8761 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     8821 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     8881 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     8941 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     9001 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     9061 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     9121 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     9181 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     9241 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     9301 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     9361 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     9421 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     9481 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     9541 ccacctgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     9601 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     9661 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     9721 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     9781 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     9841 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     9901 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     9961 aaatttaacg cgaattttaa caaaatatta acgtttacaa tttcccattc gccattcagg
    10021 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg cca
//
