LOCUS       sequence-52183-e       10001 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10001)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB018 / LRRK2_BC_1333_2187
FEATURES             Location/Qualifiers
     source          1..10001
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     329..1156
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             329..453
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(491..1096)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     CDS             complement(1167..2978)
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGQQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFSLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     promoter        complement(2987..3302)
                     /gene="Autographa californica p6.9"
                     /label=p6.9 promoter
                     /note="baculovirus late basic protein promoter"
     promoter        3317..3408
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     3333..3350
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             3421..3444
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             3445..3462
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             6051..6068
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_recomb     6105..7462
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             complement(6121..7462)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     primer_bind     complement(7554..7571)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7725..8313)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7805..7824)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8484..9275,9276..9344))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9107..9126
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9345..9449)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9476..9931
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9563..9582)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9773..9794
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag
       61 agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac
      121 aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat
      181 gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg
      241 gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac
      301 cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat
      361 aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac
      421 tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat
      481 ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa
      541 ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac
      601 agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt
      661 cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt
      721 atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt
      781 ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat
      841 tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt
      901 tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa
      961 ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt
     1021 taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt
     1081 taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta
     1141 tcaatatata gttgctgatc agatcctcat tgtttgcctc cctgctgcgg tttttcaccg
     1201 aagttcatgc cagtccagcg tttttgcagc agaaaagccg ccgacttcgg tttgcggtcg
     1261 cgagtgaaga tccctttctt gttaccgcca acgcgcaata tgccttgcga ggtcgcaaaa
     1321 tcggcgaaat tccatacctg ttcaccgacg acggcgctga cgcgatcaaa gacgcggtga
     1381 tacatatcca gccatgcaca ctgatactct tcactccaca tgtcggtgta cattgagtgc
     1441 agcccggcta acgtatccac gccgtattcg gtgatgataa tcggctgatg cagtttctcc
     1501 tgccaggcca gaagttcttt ttccagtacc ttctctgccg tttccaaatc gccgctttgg
     1561 acataccatc cgtaataacg gttcaggcac agcacatcaa agagatcgct gatggtatcg
     1621 gtgtgagcgt cgcagaacat tacattgacg caggtgatcg gacgcgtcgg gtcgagttta
     1681 cgcgttgctt ccgccagtgg cgcgaaatat tcccgtgcac cttgcggacg ggtatccggt
     1741 tcgttggcaa tactccacat caccacgctt gggtggtttt tgtcacgcgc tatcagctct
     1801 ttaatcgcct gtaagtgcgc ttgctgagtt tccccgttga ctgcctcttc gctgtacagt
     1861 tctttcggct tgttgcccgc ttcgaaacca atgcctaaag agagcgaaaa gccgacagca
     1921 gcagtttcat caatcaccac gatgccatgt tcatctgccc agtcgagcat ctcttcagcg
     1981 taagggtaat gcgaggtacg gtaggagttg gccccaatcc agtccattaa tgcgtggtcg
     2041 tgcaccatca gcacgttatc gaatcctttg ccacgtaagt ccgcatcttc atgacgacca
     2101 aagccagtaa agtagaacgg tttgtggtta atcaggaact gttggccctt cactgccact
     2161 gaccggatgc cgacgcgaag cgggtagata tcacactctg tctggctttt ggctgtgacg
     2221 cacagttcat agagataacc ttcacccggt tgccagaggt gcggattcac cacttgcaaa
     2281 gtcccgctag tgccttgtcc agttgcaacc acctgttgat ccgcatcacg cagttcaacg
     2341 ctgacatcac cattggccac cacctgccag tcaacagacg cgtggttaca gtcttgcgcg
     2401 acatgcgtca ccacggtgat atcgtccacc caggtgttcg gcgtggtgta gagcattacg
     2461 ctgcgatgga ttccggcata gttaaagaaa tcatggaagt aagactgctt tttcttgccg
     2521 ttttcgtcgg taatcaccat tcccggcggg atagtctgcc agttcagttc gttgttcaca
     2581 caaacggtga tacgtacact tttcccggca ataacatacg gcgtgacatc ggcttcaaat
     2641 ggcgtatagc cgccctgatg ctccatcact tcctgattat tgacccacac tttgccgtaa
     2701 tgagtgaccg catcgaaacg cagcacgata cgctggcctg cccaaccttt cggtataaag
     2761 acttcgcgct gataccagac gttgcccgca taattacgaa tatctgcatc ggcgaactga
     2821 tcgttaaaac tgcctggcac agcaattgcc cggctttctt gtaacgcgct ttcccaccaa
     2881 cgctgatcaa ttccacagtt ttcgcgatcc agactgaatg cccacaggcc gtcgagtttt
     2941 ttgatttcac gggttggggt ttctacagga cggaccatat ttataggtgt aatttatgta
     3001 gctgtaattt ttaccttatt aatatttttt acgctttgca ttcgacgact gaactcccaa
     3061 atatatgttt aactcgtctt ggtcgtttga atttttgttg ctgtgtttcc taatattttc
     3121 catcacctta aatatgttat tgtaatcctc aatgttgaac ttgcaattgg acacggcata
     3181 gttttccata gtcgtgtaaa acatggtatt ggctgcattg taatacatcc gactgagcgg
     3241 gtacggatct atgtgtttga gcagcctgtt caaaaactct gcatcgtcgc aaaacggaat
     3301 ttcgtcgaca gatctgatca tggagataat taaaatgata accatctcgc aaataaataa
     3361 gtattttact gttttcgtaa cagttttgta ataaaaaaac ctataaatat aggatctatg
     3421 caccaccatc accaccatca ccacttggtg ccccgcggat ccaatcgcat gaagctgatg
     3481 atcgtcggga acaccggttc tggaaagaca accctcttgc agcagcttat gaagactaag
     3541 aagtccgact tggggatgca gagcgccact gtggggatcg acgtgaaaga ctggccaatc
     3601 caaatccgtg acaagcgcaa gcgtgactta gtactgaacg tgtgggactt tgccggcagg
     3661 gaagagttct actctacgca tcctcacttc atgactcagc gtgccctcta tctggcagtt
     3721 tacgacctct caaagggcca ggctgaggtg gacgccatga aaccctggct gtttaacatc
     3781 aaggctcgtg cttccagctc ccctgtgatc ttagtcggga cacacttgga tgtttctgat
     3841 gagaagcagc gtaaggcatg tatgagtaag atcaccaaag agctgttgaa caaacgcggc
     3901 tttcccgcca tccgtgatta ccacttcgtc aacgctaccg aggaaagcga cgcactggcc
     3961 aagttacgta agaccattat caacgaaagc ctgaacttca aaattcgcga ccagctggtg
     4021 gtcggacagc tgatccccga ctgctacgtg gaacttgaga agatcatcct gagcgagcgc
     4081 aagaatgtgc ctatcgagtt tccagtgatc gatcgcaaac gtctcctcca gctcgtgcgt
     4141 gagaatcagc tgcagctcga cgagaacgag ctgcctcatg ctgtgcactt ccttaacgag
     4201 tcaggtgtcc tcctgcactt ccaggacccg gctctccagc tgagtgacct ctatttcgtg
     4261 gagccaaagt ggctctgtaa aattatggct caaatcctca ctgtcaaggt agagggctgt
     4321 ccaaagcacc caaagggcat catctcgcgt cgcgacgtgg aaaagtttct ctctaagaag
     4381 cgcaagtttc ccaagaacta catgagccag tactttaaac tgttggagaa gttccagatc
     4441 gctctgccta tcggcgaaga gtacctgttg gttccttcct ccctgtccga tcaccgcccc
     4501 gtgattgagc tgcctcactg cgagaacagc gagattatca ttcgcctata cgagatgccg
     4561 tactttccga tgggcttctg gtcaaggctc atcaatcgtc tgcttgaaat ctccccatat
     4621 atgctgagcg gtcgtgaacg cgctctgagg ccaaaccgga tgtactggcg ccaggggatt
     4681 tacttgaact ggtcccctga ggcttattgt ctggtgggct cggaagtcct ggataaccac
     4741 cccgaatcat tcctgaagat caccgttcct tcctgccgta agggttgcat tctattggga
     4801 caggtggtgg atcatatcga ttccctgatg gaggagtggt tccctggcct gttggagatc
     4861 gacatctgtg gcgagggtga gaccctgctg aagaagtggg cactctacag tttcaatgac
     4921 ggcgaagaac accagaagat tctgctcgac gacctcatga agaaggccga ggagggcgac
     4981 ctgctcgtga accccgacca gcctaggctg accatcccca tctcccagat tgcacccgac
     5041 cttatcctcg ctgacctgcc tcgcaacatc atgctgaaca atgacgagct ggagtttgaa
     5101 caagcacccg agttcctgct aggcgacggc tctttcggtt cagtttaccg tgctgcttac
     5161 gagggtgaag aggttgccgt gaagattttt aacaagcaca cttcgctccg tctgctgcgt
     5221 caggagcttg tggttctctg ccacctgcac cacccttccc tgatctccct gctggccgcc
     5281 ggcattaggc ctcgcatgct agtgatggaa ctggccagta agggttcatt ggaccgcctg
     5341 ctgcagcagg acaaggctag cctgacccgt acgctccagc accgtatcgc cctgcatgtg
     5401 gccgacggac tgcgctactt gcacagtgcc atgatcattt atcgcgatct gaagcctcat
     5461 aatgttcttc tgttcacgct ctacccaaac gcagctatca ttgccaaaat tgccgattac
     5521 ggcatagccc agtactgctg tcgtatgggt atcaagacta gcgagggtac acctgggttc
     5581 cgcgctcccg aagtcgcacg cgggaacgtg atctacaacc agcaggccga cgtgtactcg
     5641 tttggcctgc tcctgtatga cattctcact accggaggcc ggattgtgga gggcctgaaa
     5701 tttcccaacg agttcgacga gctggagatc cagggcaagc tgcctgaccc cgtgaaggag
     5761 tacggttgtg caccctggcc tatggtggag aagctcatta agcagtgtct gaaggagaac
     5821 cctcaggaac gccccacaag cgcccaggtg ttcgacattc taaactctgc agagttagtg
     5881 tgtctgacta ggcgtatctt actcccgaag aacgtgatcg ttgagtgcat ggtggcaacg
     5941 caccacaact cgcgtaacgc tagcatctgg ttaggatgtg ggcataccga tcgtggccag
     6001 ctgagtttcc tcgacctcaa caccgagtaa agcttgcggc cgcactcgag caccaccacc
     6061 accaccacta acctaggtag ctgagcgcat gcaagctgat ccgggttatt agtacattta
     6121 ttaagcgcta gattctgtgc gttgttgatt tacagacaat tgttgtacgt attttaataa
     6181 ttcattaaat ttataatctt tagggtggta tgttagagcg aaaatcaaat gattttcagc
     6241 gtctttatat ctgaatttaa atattaaatc ctcaatagat ttgtaaaata ggtttcgatt
     6301 agtttcaaac aagggttgtt tttccgaacc gatggctgga ctatctaatg gattttcgct
     6361 caacgccaca aaacttgcca aatcttgtag cagcaatcta gctttgtcga tattcgtttg
     6421 tgttttgttt tgtaataaag gttcgacgtc gttcaaaata ttatgcgctt ttgtatttct
     6481 ttcatcactg tcgttagtgt acaattgact cgacgtaaac acgttaaata gagcttggac
     6541 atatttaaca tcgggcgtgt tagctttatt aggccgatta tcgtcgtcgt cccaaccctc
     6601 gtcgttagaa gttgcttccg aagacgattt tgccatagcc acacgacgcc tattaattgt
     6661 gtcggctaac acgtccgcga tcaaatttgt agttgagctt tttggaatta tttctgattg
     6721 cgggcgtttt tgggcgggtt tcaatctaac tgtgcccgat tttaattcag acaacacgtt
     6781 agaaagcgat ggtgcaggcg gtggtaacat ttcagacggc aaatctacta atggcggcgg
     6841 tggtggagct gatgataaat ctaccatcgg tggaggcgca ggcggggctg gcggcggagg
     6901 cggaggcgga ggtggtggcg gtgatgcaga cggcggttta ggctcaaatg tctctttagg
     6961 caacacagtc ggcacctcaa ctattgtact ggtttcgggc gccgtttttg gtttgaccgg
     7021 tctgagacga gtgcgatttt tttcgtttct aatagcttcc aacaattgtt gtctgtcgtc
     7081 taaaggtgca gcgggttgag gttccgtcgg cattggtgga gcgggcggca attcagacat
     7141 cgatggtggt ggtggtggtg gaggcgctgg aatgttaggc acgggagaag gtggtggcgg
     7201 cggtgccgcc ggtataattt gttctggttt agtttgttcg cgcacgattg tgggcaccgg
     7261 cgcaggcgcc gctggctgca caacggaagg tcgtctgctt cgaggcagcg cttggggtgg
     7321 tggcaattca atattataat tggaatacaa atcgtaaaaa tctgctataa gcattgtaat
     7381 ttcgctatcg tttaccgtgc cgatatttaa caaccgctca atgtaagcaa ttgtattgta
     7441 aagagattgt ctcaagctcg gatcgatccc gcacgccgat aacaagcctt ttcattttta
     7501 ctacagcatt gtagtggcga gacacttcgc tgtcgtcgag gtttaaacgc ttcctcgctc
     7561 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     7621 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     7681 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     7741 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     7801 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     7861 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     7921 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     7981 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     8041 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     8101 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     8161 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     8221 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     8281 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     8341 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     8401 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     8461 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     8521 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     8581 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     8641 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     8701 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     8761 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     8821 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     8881 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     8941 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     9001 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     9061 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     9121 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     9181 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     9241 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     9301 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     9361 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     9421 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg
     9481 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     9541 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     9601 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     9661 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     9721 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     9781 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     9841 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     9901 aattttaaca aaatattaac gtttacaatt tcccattcgc cattcaggct gcgcaactgt
     9961 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc a
//
