LOCUS       sequence-52184-e       10070 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10070)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB018 / LRRK2_BC_1333_2210
FEATURES             Location/Qualifiers
     source          1..10070
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     329..1156
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             329..453
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(491..1096)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     CDS             complement(1167..2978)
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGQQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFSLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     promoter        complement(2987..3302)
                     /gene="Autographa californica p6.9"
                     /label=p6.9 promoter
                     /note="baculovirus late basic protein promoter"
     promoter        3317..3408
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     3333..3350
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             3421..3444
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             3445..3462
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             6120..6137
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_recomb     6174..7531
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             complement(6190..7531)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     primer_bind     complement(7623..7640)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7794..8382)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7874..7893)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8553..9344,9345..9413))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9176..9195
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9414..9518)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9545..10000
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9632..9651)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9842..9863
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag
       61 agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac
      121 aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat
      181 gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg
      241 gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac
      301 cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat
      361 aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac
      421 tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat
      481 ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa
      541 ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac
      601 agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt
      661 cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt
      721 atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt
      781 ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat
      841 tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt
      901 tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa
      961 ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt
     1021 taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt
     1081 taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta
     1141 tcaatatata gttgctgatc agatcctcat tgtttgcctc cctgctgcgg tttttcaccg
     1201 aagttcatgc cagtccagcg tttttgcagc agaaaagccg ccgacttcgg tttgcggtcg
     1261 cgagtgaaga tccctttctt gttaccgcca acgcgcaata tgccttgcga ggtcgcaaaa
     1321 tcggcgaaat tccatacctg ttcaccgacg acggcgctga cgcgatcaaa gacgcggtga
     1381 tacatatcca gccatgcaca ctgatactct tcactccaca tgtcggtgta cattgagtgc
     1441 agcccggcta acgtatccac gccgtattcg gtgatgataa tcggctgatg cagtttctcc
     1501 tgccaggcca gaagttcttt ttccagtacc ttctctgccg tttccaaatc gccgctttgg
     1561 acataccatc cgtaataacg gttcaggcac agcacatcaa agagatcgct gatggtatcg
     1621 gtgtgagcgt cgcagaacat tacattgacg caggtgatcg gacgcgtcgg gtcgagttta
     1681 cgcgttgctt ccgccagtgg cgcgaaatat tcccgtgcac cttgcggacg ggtatccggt
     1741 tcgttggcaa tactccacat caccacgctt gggtggtttt tgtcacgcgc tatcagctct
     1801 ttaatcgcct gtaagtgcgc ttgctgagtt tccccgttga ctgcctcttc gctgtacagt
     1861 tctttcggct tgttgcccgc ttcgaaacca atgcctaaag agagcgaaaa gccgacagca
     1921 gcagtttcat caatcaccac gatgccatgt tcatctgccc agtcgagcat ctcttcagcg
     1981 taagggtaat gcgaggtacg gtaggagttg gccccaatcc agtccattaa tgcgtggtcg
     2041 tgcaccatca gcacgttatc gaatcctttg ccacgtaagt ccgcatcttc atgacgacca
     2101 aagccagtaa agtagaacgg tttgtggtta atcaggaact gttggccctt cactgccact
     2161 gaccggatgc cgacgcgaag cgggtagata tcacactctg tctggctttt ggctgtgacg
     2221 cacagttcat agagataacc ttcacccggt tgccagaggt gcggattcac cacttgcaaa
     2281 gtcccgctag tgccttgtcc agttgcaacc acctgttgat ccgcatcacg cagttcaacg
     2341 ctgacatcac cattggccac cacctgccag tcaacagacg cgtggttaca gtcttgcgcg
     2401 acatgcgtca ccacggtgat atcgtccacc caggtgttcg gcgtggtgta gagcattacg
     2461 ctgcgatgga ttccggcata gttaaagaaa tcatggaagt aagactgctt tttcttgccg
     2521 ttttcgtcgg taatcaccat tcccggcggg atagtctgcc agttcagttc gttgttcaca
     2581 caaacggtga tacgtacact tttcccggca ataacatacg gcgtgacatc ggcttcaaat
     2641 ggcgtatagc cgccctgatg ctccatcact tcctgattat tgacccacac tttgccgtaa
     2701 tgagtgaccg catcgaaacg cagcacgata cgctggcctg cccaaccttt cggtataaag
     2761 acttcgcgct gataccagac gttgcccgca taattacgaa tatctgcatc ggcgaactga
     2821 tcgttaaaac tgcctggcac agcaattgcc cggctttctt gtaacgcgct ttcccaccaa
     2881 cgctgatcaa ttccacagtt ttcgcgatcc agactgaatg cccacaggcc gtcgagtttt
     2941 ttgatttcac gggttggggt ttctacagga cggaccatat ttataggtgt aatttatgta
     3001 gctgtaattt ttaccttatt aatatttttt acgctttgca ttcgacgact gaactcccaa
     3061 atatatgttt aactcgtctt ggtcgtttga atttttgttg ctgtgtttcc taatattttc
     3121 catcacctta aatatgttat tgtaatcctc aatgttgaac ttgcaattgg acacggcata
     3181 gttttccata gtcgtgtaaa acatggtatt ggctgcattg taatacatcc gactgagcgg
     3241 gtacggatct atgtgtttga gcagcctgtt caaaaactct gcatcgtcgc aaaacggaat
     3301 ttcgtcgaca gatctgatca tggagataat taaaatgata accatctcgc aaataaataa
     3361 gtattttact gttttcgtaa cagttttgta ataaaaaaac ctataaatat aggatctatg
     3421 caccaccatc accaccatca ccacttggtg ccccgcggat ccaatcgcat gaagctgatg
     3481 atcgtcggga acaccggttc tggaaagaca accctcttgc agcagcttat gaagactaag
     3541 aagtccgact tggggatgca gagcgccact gtggggatcg acgtgaaaga ctggccaatc
     3601 caaatccgtg acaagcgcaa gcgtgactta gtactgaacg tgtgggactt tgccggcagg
     3661 gaagagttct actctacgca tcctcacttc atgactcagc gtgccctcta tctggcagtt
     3721 tacgacctct caaagggcca ggctgaggtg gacgccatga aaccctggct gtttaacatc
     3781 aaggctcgtg cttccagctc ccctgtgatc ttagtcggga cacacttgga tgtttctgat
     3841 gagaagcagc gtaaggcatg tatgagtaag atcaccaaag agctgttgaa caaacgcggc
     3901 tttcccgcca tccgtgatta ccacttcgtc aacgctaccg aggaaagcga cgcactggcc
     3961 aagttacgta agaccattat caacgaaagc ctgaacttca aaattcgcga ccagctggtg
     4021 gtcggacagc tgatccccga ctgctacgtg gaacttgaga agatcatcct gagcgagcgc
     4081 aagaatgtgc ctatcgagtt tccagtgatc gatcgcaaac gtctcctcca gctcgtgcgt
     4141 gagaatcagc tgcagctcga cgagaacgag ctgcctcatg ctgtgcactt ccttaacgag
     4201 tcaggtgtcc tcctgcactt ccaggacccg gctctccagc tgagtgacct ctatttcgtg
     4261 gagccaaagt ggctctgtaa aattatggct caaatcctca ctgtcaaggt agagggctgt
     4321 ccaaagcacc caaagggcat catctcgcgt cgcgacgtgg aaaagtttct ctctaagaag
     4381 cgcaagtttc ccaagaacta catgagccag tactttaaac tgttggagaa gttccagatc
     4441 gctctgccta tcggcgaaga gtacctgttg gttccttcct ccctgtccga tcaccgcccc
     4501 gtgattgagc tgcctcactg cgagaacagc gagattatca ttcgcctata cgagatgccg
     4561 tactttccga tgggcttctg gtcaaggctc atcaatcgtc tgcttgaaat ctccccatat
     4621 atgctgagcg gtcgtgaacg cgctctgagg ccaaaccgga tgtactggcg ccaggggatt
     4681 tacttgaact ggtcccctga ggcttattgt ctggtgggct cggaagtcct ggataaccac
     4741 cccgaatcat tcctgaagat caccgttcct tcctgccgta agggttgcat tctattggga
     4801 caggtggtgg atcatatcga ttccctgatg gaggagtggt tccctggcct gttggagatc
     4861 gacatctgtg gcgagggtga gaccctgctg aagaagtggg cactctacag tttcaatgac
     4921 ggcgaagaac accagaagat tctgctcgac gacctcatga agaaggccga ggagggcgac
     4981 ctgctcgtga accccgacca gcctaggctg accatcccca tctcccagat tgcacccgac
     5041 cttatcctcg ctgacctgcc tcgcaacatc atgctgaaca atgacgagct ggagtttgaa
     5101 caagcacccg agttcctgct aggcgacggc tctttcggtt cagtttaccg tgctgcttac
     5161 gagggtgaag aggttgccgt gaagattttt aacaagcaca cttcgctccg tctgctgcgt
     5221 caggagcttg tggttctctg ccacctgcac cacccttccc tgatctccct gctggccgcc
     5281 ggcattaggc ctcgcatgct agtgatggaa ctggccagta agggttcatt ggaccgcctg
     5341 ctgcagcagg acaaggctag cctgacccgt acgctccagc accgtatcgc cctgcatgtg
     5401 gccgacggac tgcgctactt gcacagtgcc atgatcattt atcgcgatct gaagcctcat
     5461 aatgttcttc tgttcacgct ctacccaaac gcagctatca ttgccaaaat tgccgattac
     5521 ggcatagccc agtactgctg tcgtatgggt atcaagacta gcgagggtac acctgggttc
     5581 cgcgctcccg aagtcgcacg cgggaacgtg atctacaacc agcaggccga cgtgtactcg
     5641 tttggcctgc tcctgtatga cattctcact accggaggcc ggattgtgga gggcctgaaa
     5701 tttcccaacg agttcgacga gctggagatc cagggcaagc tgcctgaccc cgtgaaggag
     5761 tacggttgtg caccctggcc tatggtggag aagctcatta agcagtgtct gaaggagaac
     5821 cctcaggaac gccccacaag cgcccaggtg ttcgacattc taaactctgc agagttagtg
     5881 tgtctgacta ggcgtatctt actcccgaag aacgtgatcg ttgagtgcat ggtggcaacg
     5941 caccacaact cgcgtaacgc tagcatctgg ttaggatgtg ggcataccga tcgtggccag
     6001 ctgagtttcc tcgacctcaa caccgagggg tatactagcg aggaggtcgc tgattcacgc
     6061 attctgtgcc tcgcccttgt ccatctgcct gttgagtaaa gcttgcggcc gcactcgagc
     6121 accaccacca ccaccactaa cctaggtagc tgagcgcatg caagctgatc cgggttatta
     6181 gtacatttat taagcgctag attctgtgcg ttgttgattt acagacaatt gttgtacgta
     6241 ttttaataat tcattaaatt tataatcttt agggtggtat gttagagcga aaatcaaatg
     6301 attttcagcg tctttatatc tgaatttaaa tattaaatcc tcaatagatt tgtaaaatag
     6361 gtttcgatta gtttcaaaca agggttgttt ttccgaaccg atggctggac tatctaatgg
     6421 attttcgctc aacgccacaa aacttgccaa atcttgtagc agcaatctag ctttgtcgat
     6481 attcgtttgt gttttgtttt gtaataaagg ttcgacgtcg ttcaaaatat tatgcgcttt
     6541 tgtatttctt tcatcactgt cgttagtgta caattgactc gacgtaaaca cgttaaatag
     6601 agcttggaca tatttaacat cgggcgtgtt agctttatta ggccgattat cgtcgtcgtc
     6661 ccaaccctcg tcgttagaag ttgcttccga agacgatttt gccatagcca cacgacgcct
     6721 attaattgtg tcggctaaca cgtccgcgat caaatttgta gttgagcttt ttggaattat
     6781 ttctgattgc gggcgttttt gggcgggttt caatctaact gtgcccgatt ttaattcaga
     6841 caacacgtta gaaagcgatg gtgcaggcgg tggtaacatt tcagacggca aatctactaa
     6901 tggcggcggt ggtggagctg atgataaatc taccatcggt ggaggcgcag gcggggctgg
     6961 cggcggaggc ggaggcggag gtggtggcgg tgatgcagac ggcggtttag gctcaaatgt
     7021 ctctttaggc aacacagtcg gcacctcaac tattgtactg gtttcgggcg ccgtttttgg
     7081 tttgaccggt ctgagacgag tgcgattttt ttcgtttcta atagcttcca acaattgttg
     7141 tctgtcgtct aaaggtgcag cgggttgagg ttccgtcggc attggtggag cgggcggcaa
     7201 ttcagacatc gatggtggtg gtggtggtgg aggcgctgga atgttaggca cgggagaagg
     7261 tggtggcggc ggtgccgccg gtataatttg ttctggttta gtttgttcgc gcacgattgt
     7321 gggcaccggc gcaggcgccg ctggctgcac aacggaaggt cgtctgcttc gaggcagcgc
     7381 ttggggtggt ggcaattcaa tattataatt ggaatacaaa tcgtaaaaat ctgctataag
     7441 cattgtaatt tcgctatcgt ttaccgtgcc gatatttaac aaccgctcaa tgtaagcaat
     7501 tgtattgtaa agagattgtc tcaagctcgg atcgatcccg cacgccgata acaagccttt
     7561 tcatttttac tacagcattg tagtggcgag acacttcgct gtcgtcgagg tttaaacgct
     7621 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     7681 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     7741 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     7801 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     7861 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     7921 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     7981 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     8041 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     8101 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     8161 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     8221 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     8281 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     8341 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     8401 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     8461 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     8521 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     8581 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     8641 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     8701 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     8761 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     8821 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     8881 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     8941 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     9001 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     9061 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     9121 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     9181 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     9241 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     9301 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     9361 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     9421 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     9481 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     9541 cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     9601 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     9661 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     9721 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     9781 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     9841 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     9901 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     9961 tttaacgcga attttaacaa aatattaacg tttacaattt cccattcgcc attcaggctg
    10021 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
//
