LOCUS       sequence-52204-e        9635 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9635)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9635)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB018 / LRRK2_BC_1500_2232
FEATURES             Location/Qualifiers
     source          1..9635
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     329..1156
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             329..453
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(491..1096)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     CDS             complement(1167..2978)
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGQQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFSLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     promoter        complement(2987..3302)
                     /gene="Autographa californica p6.9"
                     /label=p6.9 promoter
                     /note="baculovirus late basic protein promoter"
     promoter        3317..3408
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     3333..3350
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             3421..3444
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             3445..3462
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             5685..5702
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_recomb     5739..7096
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             complement(5755..7096)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     primer_bind     complement(7188..7205)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7359..7947)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7439..7458)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8118..8909,8910..8978))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8741..8760
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8979..9083)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9110..9565
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9197..9216)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9407..9428
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag
       61 agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac
      121 aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat
      181 gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg
      241 gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac
      301 cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat
      361 aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac
      421 tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat
      481 ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa
      541 ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac
      601 agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt
      661 cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt
      721 atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt
      781 ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat
      841 tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt
      901 tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa
      961 ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt
     1021 taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt
     1081 taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta
     1141 tcaatatata gttgctgatc agatcctcat tgtttgcctc cctgctgcgg tttttcaccg
     1201 aagttcatgc cagtccagcg tttttgcagc agaaaagccg ccgacttcgg tttgcggtcg
     1261 cgagtgaaga tccctttctt gttaccgcca acgcgcaata tgccttgcga ggtcgcaaaa
     1321 tcggcgaaat tccatacctg ttcaccgacg acggcgctga cgcgatcaaa gacgcggtga
     1381 tacatatcca gccatgcaca ctgatactct tcactccaca tgtcggtgta cattgagtgc
     1441 agcccggcta acgtatccac gccgtattcg gtgatgataa tcggctgatg cagtttctcc
     1501 tgccaggcca gaagttcttt ttccagtacc ttctctgccg tttccaaatc gccgctttgg
     1561 acataccatc cgtaataacg gttcaggcac agcacatcaa agagatcgct gatggtatcg
     1621 gtgtgagcgt cgcagaacat tacattgacg caggtgatcg gacgcgtcgg gtcgagttta
     1681 cgcgttgctt ccgccagtgg cgcgaaatat tcccgtgcac cttgcggacg ggtatccggt
     1741 tcgttggcaa tactccacat caccacgctt gggtggtttt tgtcacgcgc tatcagctct
     1801 ttaatcgcct gtaagtgcgc ttgctgagtt tccccgttga ctgcctcttc gctgtacagt
     1861 tctttcggct tgttgcccgc ttcgaaacca atgcctaaag agagcgaaaa gccgacagca
     1921 gcagtttcat caatcaccac gatgccatgt tcatctgccc agtcgagcat ctcttcagcg
     1981 taagggtaat gcgaggtacg gtaggagttg gccccaatcc agtccattaa tgcgtggtcg
     2041 tgcaccatca gcacgttatc gaatcctttg ccacgtaagt ccgcatcttc atgacgacca
     2101 aagccagtaa agtagaacgg tttgtggtta atcaggaact gttggccctt cactgccact
     2161 gaccggatgc cgacgcgaag cgggtagata tcacactctg tctggctttt ggctgtgacg
     2221 cacagttcat agagataacc ttcacccggt tgccagaggt gcggattcac cacttgcaaa
     2281 gtcccgctag tgccttgtcc agttgcaacc acctgttgat ccgcatcacg cagttcaacg
     2341 ctgacatcac cattggccac cacctgccag tcaacagacg cgtggttaca gtcttgcgcg
     2401 acatgcgtca ccacggtgat atcgtccacc caggtgttcg gcgtggtgta gagcattacg
     2461 ctgcgatgga ttccggcata gttaaagaaa tcatggaagt aagactgctt tttcttgccg
     2521 ttttcgtcgg taatcaccat tcccggcggg atagtctgcc agttcagttc gttgttcaca
     2581 caaacggtga tacgtacact tttcccggca ataacatacg gcgtgacatc ggcttcaaat
     2641 ggcgtatagc cgccctgatg ctccatcact tcctgattat tgacccacac tttgccgtaa
     2701 tgagtgaccg catcgaaacg cagcacgata cgctggcctg cccaaccttt cggtataaag
     2761 acttcgcgct gataccagac gttgcccgca taattacgaa tatctgcatc ggcgaactga
     2821 tcgttaaaac tgcctggcac agcaattgcc cggctttctt gtaacgcgct ttcccaccaa
     2881 cgctgatcaa ttccacagtt ttcgcgatcc agactgaatg cccacaggcc gtcgagtttt
     2941 ttgatttcac gggttggggt ttctacagga cggaccatat ttataggtgt aatttatgta
     3001 gctgtaattt ttaccttatt aatatttttt acgctttgca ttcgacgact gaactcccaa
     3061 atatatgttt aactcgtctt ggtcgtttga atttttgttg ctgtgtttcc taatattttc
     3121 catcacctta aatatgttat tgtaatcctc aatgttgaac ttgcaattgg acacggcata
     3181 gttttccata gtcgtgtaaa acatggtatt ggctgcattg taatacatcc gactgagcgg
     3241 gtacggatct atgtgtttga gcagcctgtt caaaaactct gcatcgtcgc aaaacggaat
     3301 ttcgtcgaca gatctgatca tggagataat taaaatgata accatctcgc aaataaataa
     3361 gtattttact gttttcgtaa cagttttgta ataaaaaaac ctataaatat aggatctatg
     3421 caccaccatc accaccatca ccacttggtg ccccgcggat ccttacgtaa gaccattatc
     3481 aacgaaagcc tgaacttcaa aattcgcgac cagctggtgg tcggacagct gatccccgac
     3541 tgctacgtgg aacttgagaa gatcatcctg agcgagcgca agaatgtgcc tatcgagttt
     3601 ccagtgatcg atcgcaaacg tctcctccag ctcgtgcgtg agaatcagct gcagctcgac
     3661 gagaacgagc tgcctcatgc tgtgcacttc cttaacgagt caggtgtcct cctgcacttc
     3721 caggacccgg ctctccagct gagtgacctc tatttcgtgg agccaaagtg gctctgtaaa
     3781 attatggctc aaatcctcac tgtcaaggta gagggctgtc caaagcaccc aaagggcatc
     3841 atctcgcgtc gcgacgtgga aaagtttctc tctaagaagc gcaagtttcc caagaactac
     3901 atgagccagt actttaaact gttggagaag ttccagatcg ctctgcctat cggcgaagag
     3961 tacctgttgg ttccttcctc cctgtccgat caccgccccg tgattgagct gcctcactgc
     4021 gagaacagcg agattatcat tcgcctatac gagatgccgt actttccgat gggcttctgg
     4081 tcaaggctca tcaatcgtct gcttgaaatc tccccatata tgctgagcgg tcgtgaacgc
     4141 gctctgaggc caaaccggat gtactggcgc caggggattt acttgaactg gtcccctgag
     4201 gcttattgtc tggtgggctc ggaagtcctg gataaccacc ccgaatcatt cctgaagatc
     4261 accgttcctt cctgccgtaa gggttgcatt ctattgggac aggtggtgga tcatatcgat
     4321 tccctgatgg aggagtggtt ccctggcctg ttggagatcg acatctgtgg cgagggtgag
     4381 accctgctga agaagtgggc actctacagt ttcaatgacg gcgaagaaca ccagaagatt
     4441 ctgctcgacg acctcatgaa gaaggccgag gagggcgacc tgctcgtgaa ccccgaccag
     4501 cctaggctga ccatccccat ctcccagatt gcacccgacc ttatcctcgc tgacctgcct
     4561 cgcaacatca tgctgaacaa tgacgagctg gagtttgaac aagcacccga gttcctgcta
     4621 ggcgacggct ctttcggttc agtttaccgt gctgcttacg agggtgaaga ggttgccgtg
     4681 aagattttta acaagcacac ttcgctccgt ctgctgcgtc aggagcttgt ggttctctgc
     4741 cacctgcacc acccttccct gatctccctg ctggccgccg gcattaggcc tcgcatgcta
     4801 gtgatggaac tggccagtaa gggttcattg gaccgcctgc tgcagcagga caaggctagc
     4861 ctgacccgta cgctccagca ccgtatcgcc ctgcatgtgg ccgacggact gcgctacttg
     4921 cacagtgcca tgatcattta tcgcgatctg aagcctcata atgttcttct gttcacgctc
     4981 tacccaaacg cagctatcat tgccaaaatt gccgattacg gcatagccca gtactgctgt
     5041 cgtatgggta tcaagactag cgagggtaca cctgggttcc gcgctcccga agtcgcacgc
     5101 gggaacgtga tctacaacca gcaggccgac gtgtactcgt ttggcctgct cctgtatgac
     5161 attctcacta ccggaggccg gattgtggag ggcctgaaat ttcccaacga gttcgacgag
     5221 ctggagatcc agggcaagct gcctgacccc gtgaaggagt acggttgtgc accctggcct
     5281 atggtggaga agctcattaa gcagtgtctg aaggagaacc ctcaggaacg ccccacaagc
     5341 gcccaggtgt tcgacattct aaactctgca gagttagtgt gtctgactag gcgtatctta
     5401 ctcccgaaga acgtgatcgt tgagtgcatg gtggcaacgc accacaactc gcgtaacgct
     5461 agcatctggt taggatgtgg gcataccgat cgtggccagc tgagtttcct cgacctcaac
     5521 accgaggggt atactagcga ggaggtcgct gattcacgca ttctgtgcct cgcccttgtc
     5581 catctgcctg ttgagaaaga atcgtggatt gttagtggca cccagagcgg gaccctcctc
     5641 gtgatcaata ccgaagacgg gtaaagcttg cggccgcact cgagcaccac caccaccacc
     5701 actaacctag gtagctgagc gcatgcaagc tgatccgggt tattagtaca tttattaagc
     5761 gctagattct gtgcgttgtt gatttacaga caattgttgt acgtatttta ataattcatt
     5821 aaatttataa tctttagggt ggtatgttag agcgaaaatc aaatgatttt cagcgtcttt
     5881 atatctgaat ttaaatatta aatcctcaat agatttgtaa aataggtttc gattagtttc
     5941 aaacaagggt tgtttttccg aaccgatggc tggactatct aatggatttt cgctcaacgc
     6001 cacaaaactt gccaaatctt gtagcagcaa tctagctttg tcgatattcg tttgtgtttt
     6061 gttttgtaat aaaggttcga cgtcgttcaa aatattatgc gcttttgtat ttctttcatc
     6121 actgtcgtta gtgtacaatt gactcgacgt aaacacgtta aatagagctt ggacatattt
     6181 aacatcgggc gtgttagctt tattaggccg attatcgtcg tcgtcccaac cctcgtcgtt
     6241 agaagttgct tccgaagacg attttgccat agccacacga cgcctattaa ttgtgtcggc
     6301 taacacgtcc gcgatcaaat ttgtagttga gctttttgga attatttctg attgcgggcg
     6361 tttttgggcg ggtttcaatc taactgtgcc cgattttaat tcagacaaca cgttagaaag
     6421 cgatggtgca ggcggtggta acatttcaga cggcaaatct actaatggcg gcggtggtgg
     6481 agctgatgat aaatctacca tcggtggagg cgcaggcggg gctggcggcg gaggcggagg
     6541 cggaggtggt ggcggtgatg cagacggcgg tttaggctca aatgtctctt taggcaacac
     6601 agtcggcacc tcaactattg tactggtttc gggcgccgtt tttggtttga ccggtctgag
     6661 acgagtgcga tttttttcgt ttctaatagc ttccaacaat tgttgtctgt cgtctaaagg
     6721 tgcagcgggt tgaggttccg tcggcattgg tggagcgggc ggcaattcag acatcgatgg
     6781 tggtggtggt ggtggaggcg ctggaatgtt aggcacggga gaaggtggtg gcggcggtgc
     6841 cgccggtata atttgttctg gtttagtttg ttcgcgcacg attgtgggca ccggcgcagg
     6901 cgccgctggc tgcacaacgg aaggtcgtct gcttcgaggc agcgcttggg gtggtggcaa
     6961 ttcaatatta taattggaat acaaatcgta aaaatctgct ataagcattg taatttcgct
     7021 atcgtttacc gtgccgatat ttaacaaccg ctcaatgtaa gcaattgtat tgtaaagaga
     7081 ttgtctcaag ctcggatcga tcccgcacgc cgataacaag ccttttcatt tttactacag
     7141 cattgtagtg gcgagacact tcgctgtcgt cgaggtttaa acgcttcctc gctcactgac
     7201 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     7261 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     7321 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     7381 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     7441 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     7501 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     7561 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     7621 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     7681 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     7741 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     7801 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     7861 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     7921 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     7981 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     8041 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     8101 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     8161 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     8221 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     8281 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     8341 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     8401 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     8461 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     8521 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     8581 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     8641 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     8701 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     8761 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     8821 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     8881 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     8941 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     9001 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     9061 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt
     9121 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     9181 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     9241 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     9301 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     9361 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     9421 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     9481 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     9541 aacaaaatat taacgtttac aatttcccat tcgccattca ggctgcgcaa ctgttgggaa
     9601 gggcgatcgg tgcgggcctc ttcgctatta cgcca
//
