LOCUS       sequence-52209-e        9479 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9479)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9479)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB018 / LRRK2_BC_1847_2527_M2397T
FEATURES             Location/Qualifiers
     source          1..9479
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     329..1156
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             329..453
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(491..1096)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     CDS             complement(1167..2978)
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGQQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFSLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     promoter        complement(2987..3302)
                     /gene="Autographa californica p6.9"
                     /label=p6.9 promoter
                     /note="baculovirus late basic protein promoter"
     promoter        3317..3408
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     3333..3350
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             3421..3444
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             3445..3462
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             5529..5546
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_recomb     5583..6940
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             complement(5599..6940)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     primer_bind     complement(7032..7049)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7203..7791)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7283..7302)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7962..8753,8754..8822))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8585..8604
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8823..8927)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8954..9409
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9041..9060)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9251..9272
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag
       61 agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac
      121 aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat
      181 gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg
      241 gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac
      301 cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat
      361 aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac
      421 tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat
      481 ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa
      541 ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac
      601 agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt
      661 cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt
      721 atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt
      781 ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat
      841 tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt
      901 tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa
      961 ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt
     1021 taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt
     1081 taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta
     1141 tcaatatata gttgctgatc agatcctcat tgtttgcctc cctgctgcgg tttttcaccg
     1201 aagttcatgc cagtccagcg tttttgcagc agaaaagccg ccgacttcgg tttgcggtcg
     1261 cgagtgaaga tccctttctt gttaccgcca acgcgcaata tgccttgcga ggtcgcaaaa
     1321 tcggcgaaat tccatacctg ttcaccgacg acggcgctga cgcgatcaaa gacgcggtga
     1381 tacatatcca gccatgcaca ctgatactct tcactccaca tgtcggtgta cattgagtgc
     1441 agcccggcta acgtatccac gccgtattcg gtgatgataa tcggctgatg cagtttctcc
     1501 tgccaggcca gaagttcttt ttccagtacc ttctctgccg tttccaaatc gccgctttgg
     1561 acataccatc cgtaataacg gttcaggcac agcacatcaa agagatcgct gatggtatcg
     1621 gtgtgagcgt cgcagaacat tacattgacg caggtgatcg gacgcgtcgg gtcgagttta
     1681 cgcgttgctt ccgccagtgg cgcgaaatat tcccgtgcac cttgcggacg ggtatccggt
     1741 tcgttggcaa tactccacat caccacgctt gggtggtttt tgtcacgcgc tatcagctct
     1801 ttaatcgcct gtaagtgcgc ttgctgagtt tccccgttga ctgcctcttc gctgtacagt
     1861 tctttcggct tgttgcccgc ttcgaaacca atgcctaaag agagcgaaaa gccgacagca
     1921 gcagtttcat caatcaccac gatgccatgt tcatctgccc agtcgagcat ctcttcagcg
     1981 taagggtaat gcgaggtacg gtaggagttg gccccaatcc agtccattaa tgcgtggtcg
     2041 tgcaccatca gcacgttatc gaatcctttg ccacgtaagt ccgcatcttc atgacgacca
     2101 aagccagtaa agtagaacgg tttgtggtta atcaggaact gttggccctt cactgccact
     2161 gaccggatgc cgacgcgaag cgggtagata tcacactctg tctggctttt ggctgtgacg
     2221 cacagttcat agagataacc ttcacccggt tgccagaggt gcggattcac cacttgcaaa
     2281 gtcccgctag tgccttgtcc agttgcaacc acctgttgat ccgcatcacg cagttcaacg
     2341 ctgacatcac cattggccac cacctgccag tcaacagacg cgtggttaca gtcttgcgcg
     2401 acatgcgtca ccacggtgat atcgtccacc caggtgttcg gcgtggtgta gagcattacg
     2461 ctgcgatgga ttccggcata gttaaagaaa tcatggaagt aagactgctt tttcttgccg
     2521 ttttcgtcgg taatcaccat tcccggcggg atagtctgcc agttcagttc gttgttcaca
     2581 caaacggtga tacgtacact tttcccggca ataacatacg gcgtgacatc ggcttcaaat
     2641 ggcgtatagc cgccctgatg ctccatcact tcctgattat tgacccacac tttgccgtaa
     2701 tgagtgaccg catcgaaacg cagcacgata cgctggcctg cccaaccttt cggtataaag
     2761 acttcgcgct gataccagac gttgcccgca taattacgaa tatctgcatc ggcgaactga
     2821 tcgttaaaac tgcctggcac agcaattgcc cggctttctt gtaacgcgct ttcccaccaa
     2881 cgctgatcaa ttccacagtt ttcgcgatcc agactgaatg cccacaggcc gtcgagtttt
     2941 ttgatttcac gggttggggt ttctacagga cggaccatat ttataggtgt aatttatgta
     3001 gctgtaattt ttaccttatt aatatttttt acgctttgca ttcgacgact gaactcccaa
     3061 atatatgttt aactcgtctt ggtcgtttga atttttgttg ctgtgtttcc taatattttc
     3121 catcacctta aatatgttat tgtaatcctc aatgttgaac ttgcaattgg acacggcata
     3181 gttttccata gtcgtgtaaa acatggtatt ggctgcattg taatacatcc gactgagcgg
     3241 gtacggatct atgtgtttga gcagcctgtt caaaaactct gcatcgtcgc aaaacggaat
     3301 ttcgtcgaca gatctgatca tggagataat taaaatgata accatctcgc aaataaataa
     3361 gtattttact gttttcgtaa cagttttgta ataaaaaaac ctataaatat aggatctatg
     3421 caccaccatc accaccatca ccacttggtg ccccgcggat ccaggctgac catccccatc
     3481 tcccagattg cacccgacct tatcctcgct gacctgcctc gcaacatcat gctgaacaat
     3541 gacgagctgg agtttgaaca agcacccgag ttcctgctag gcgacggctc tttcggttca
     3601 gtttaccgtg ctgcttacga gggtgaagag gttgccgtga agatttttaa caagcacact
     3661 tcgctccgtc tgctgcgtca ggagcttgtg gttctctgcc acctgcacca cccttccctg
     3721 atctccctgc tggccgccgg cattaggcct cgcatgctag tgatggaact ggccagtaag
     3781 ggttcattgg accgcctgct gcagcaggac aaggctagcc tgacccgtac gctccagcac
     3841 cgtatcgccc tgcatgtggc cgacggactg cgctacttgc acagtgccat gatcatttat
     3901 cgcgatctga agcctcataa tgttcttctg ttcacgctct acccaaacgc agctatcatt
     3961 gccaaaattg ccgattacgg catagcccag tactgctgtc gtatgggtat caagactagc
     4021 gagggtacac ctgggttccg cgctcccgaa gtcgcacgcg ggaacgtgat ctacaaccag
     4081 caggccgacg tgtactcgtt tggcctgctc ctgtatgaca ttctcactac cggaggccgg
     4141 attgtggagg gcctgaaatt tcccaacgag ttcgacgagc tggagatcca gggcaagctg
     4201 cctgaccccg tgaaggagta cggttgtgca ccctggccta tggtggagaa gctcattaag
     4261 cagtgtctga aggagaaccc tcaggaacgc cccacaagcg cccaggtgtt cgacattcta
     4321 aactctgcag agttagtgtg tctgactagg cgtatcttac tcccgaagaa cgtgatcgtt
     4381 gagtgcatgg tggcaacgca ccacaactcg cgtaacgcta gcatctggtt aggatgtggg
     4441 cataccgatc gtggccagct gagtttcctc gacctcaaca ccgaggggta tactagcgag
     4501 gaggtcgctg attcacgcat tctgtgcctc gcccttgtcc atctgcctgt tgagaaagaa
     4561 tcgtggattg ttagtggcac ccagagcggg accctcctcg tgatcaatac cgaagacggg
     4621 aagaagcgtc acactctcga aaagatgact gactccgtga cctgtctgta ctgcaattcc
     4681 ttctccaagc aaagcaagca gaagaatttc ctgctcgtag ggaccgctga cggaaaactg
     4741 gcaatcttcg aggacaaaac cgtgaagttg aagggtgccg caccccttaa gatcctgaac
     4801 attggcaacg ttagcacccc tctgatgtgt ctatcagaat caactaactc caccgagcgc
     4861 aatgtgatgt ggggcgggtg cggcaccaag attttctcct tcagcaacga cttcaccatc
     4921 cagaagctga tcgagacccg taccagtcag ctcttcagtt acgccgcctt ctccgactca
     4981 aacataatca ccgtggtcgt tgacaccgca ctgtatatcg caaagcagaa cagccccgtc
     5041 gtggaagtgt gggataagaa aactgagaag ctctgcggcc taatcgattg tgtgcacttc
     5101 cttcgcgagg tgaccgttaa agagaataaa gagagcaagc acaagatgag ctactcgggt
     5161 agggtgaaga ccctctgtct ccagaagaac accgcactct ggattggcac cggcgggggc
     5221 cacatcctgc tcttagacct gagtactagg cgtttgatcc gtgtaattta caacttttgt
     5281 aacagtgttc gtgtaatgat gaccgcccag cttggctcgc tcaagaacgt catgctggtg
     5341 ctaggctaca accgcaagaa cactgagggg actcagaagc agaaggaaat ccagagttgt
     5401 ctgaccgttt gggacattaa cctgcctcac gaggtccaaa atctggaaaa gcatatcgag
     5461 gtccgtaaag agctggccga gaagatgagg cgaacctcag ttgaataaag cttgcggccg
     5521 cactcgagca ccaccaccac caccactaac ctaggtagct gagcgcatgc aagctgatcc
     5581 gggttattag tacatttatt aagcgctaga ttctgtgcgt tgttgattta cagacaattg
     5641 ttgtacgtat tttaataatt cattaaattt ataatcttta gggtggtatg ttagagcgaa
     5701 aatcaaatga ttttcagcgt ctttatatct gaatttaaat attaaatcct caatagattt
     5761 gtaaaatagg tttcgattag tttcaaacaa gggttgtttt tccgaaccga tggctggact
     5821 atctaatgga ttttcgctca acgccacaaa acttgccaaa tcttgtagca gcaatctagc
     5881 tttgtcgata ttcgtttgtg ttttgttttg taataaaggt tcgacgtcgt tcaaaatatt
     5941 atgcgctttt gtatttcttt catcactgtc gttagtgtac aattgactcg acgtaaacac
     6001 gttaaataga gcttggacat atttaacatc gggcgtgtta gctttattag gccgattatc
     6061 gtcgtcgtcc caaccctcgt cgttagaagt tgcttccgaa gacgattttg ccatagccac
     6121 acgacgccta ttaattgtgt cggctaacac gtccgcgatc aaatttgtag ttgagctttt
     6181 tggaattatt tctgattgcg ggcgtttttg ggcgggtttc aatctaactg tgcccgattt
     6241 taattcagac aacacgttag aaagcgatgg tgcaggcggt ggtaacattt cagacggcaa
     6301 atctactaat ggcggcggtg gtggagctga tgataaatct accatcggtg gaggcgcagg
     6361 cggggctggc ggcggaggcg gaggcggagg tggtggcggt gatgcagacg gcggtttagg
     6421 ctcaaatgtc tctttaggca acacagtcgg cacctcaact attgtactgg tttcgggcgc
     6481 cgtttttggt ttgaccggtc tgagacgagt gcgatttttt tcgtttctaa tagcttccaa
     6541 caattgttgt ctgtcgtcta aaggtgcagc gggttgaggt tccgtcggca ttggtggagc
     6601 gggcggcaat tcagacatcg atggtggtgg tggtggtgga ggcgctggaa tgttaggcac
     6661 gggagaaggt ggtggcggcg gtgccgccgg tataatttgt tctggtttag tttgttcgcg
     6721 cacgattgtg ggcaccggcg caggcgccgc tggctgcaca acggaaggtc gtctgcttcg
     6781 aggcagcgct tggggtggtg gcaattcaat attataattg gaatacaaat cgtaaaaatc
     6841 tgctataagc attgtaattt cgctatcgtt taccgtgccg atatttaaca accgctcaat
     6901 gtaagcaatt gtattgtaaa gagattgtct caagctcgga tcgatcccgc acgccgataa
     6961 caagcctttt catttttact acagcattgt agtggcgaga cacttcgctg tcgtcgaggt
     7021 ttaaacgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     7081 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     7141 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     7201 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     7261 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     7321 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     7381 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     7441 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     7501 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     7561 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     7621 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     7681 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     7741 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     7801 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     7861 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     7921 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     7981 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     8041 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     8101 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     8161 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     8221 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     8281 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     8341 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     8401 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     8461 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     8521 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     8581 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     8641 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     8701 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     8761 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     8821 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     8881 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     8941 aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     9001 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     9061 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     9121 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     9181 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     9241 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     9301 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     9361 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc ccattcgcca
     9421 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgcca
//
