LOCUS       sequence-52230-e       10077 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10077)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB36(revcomp)/Dvl1_1_670
FEATURES             Location/Qualifiers
     source          1..10077
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     329..1156
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             329..453
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(491..1096)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     CDS             complement(1167..2978)
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGQQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFSLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     promoter        complement(2987..3302)
                     /gene="Autographa californica p6.9"
                     /label=p6.9 promoter
                     /note="baculovirus late basic protein promoter"
     promoter        complement(3400..3509)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        complement(3660..3751)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     promoter        3776..3885
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        3991..4082
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             6127..6144
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_recomb     6181..7538
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             complement(6197..7538)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     primer_bind     complement(7630..7647)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7801..8389)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7881..7900)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8560..9351,9352..9420))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9183..9202
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9421..9525)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9552..10007
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9639..9658)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9849..9870
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag
       61 agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac
      121 aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat
      181 gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg
      241 gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac
      301 cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat
      361 aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac
      421 tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat
      481 ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa
      541 ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac
      601 agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt
      661 cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt
      721 atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt
      781 ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat
      841 tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt
      901 tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa
      961 ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt
     1021 taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt
     1081 taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta
     1141 tcaatatata gttgctgatc agatcctcat tgtttgcctc cctgctgcgg tttttcaccg
     1201 aagttcatgc cagtccagcg tttttgcagc agaaaagccg ccgacttcgg tttgcggtcg
     1261 cgagtgaaga tccctttctt gttaccgcca acgcgcaata tgccttgcga ggtcgcaaaa
     1321 tcggcgaaat tccatacctg ttcaccgacg acggcgctga cgcgatcaaa gacgcggtga
     1381 tacatatcca gccatgcaca ctgatactct tcactccaca tgtcggtgta cattgagtgc
     1441 agcccggcta acgtatccac gccgtattcg gtgatgataa tcggctgatg cagtttctcc
     1501 tgccaggcca gaagttcttt ttccagtacc ttctctgccg tttccaaatc gccgctttgg
     1561 acataccatc cgtaataacg gttcaggcac agcacatcaa agagatcgct gatggtatcg
     1621 gtgtgagcgt cgcagaacat tacattgacg caggtgatcg gacgcgtcgg gtcgagttta
     1681 cgcgttgctt ccgccagtgg cgcgaaatat tcccgtgcac cttgcggacg ggtatccggt
     1741 tcgttggcaa tactccacat caccacgctt gggtggtttt tgtcacgcgc tatcagctct
     1801 ttaatcgcct gtaagtgcgc ttgctgagtt tccccgttga ctgcctcttc gctgtacagt
     1861 tctttcggct tgttgcccgc ttcgaaacca atgcctaaag agagcgaaaa gccgacagca
     1921 gcagtttcat caatcaccac gatgccatgt tcatctgccc agtcgagcat ctcttcagcg
     1981 taagggtaat gcgaggtacg gtaggagttg gccccaatcc agtccattaa tgcgtggtcg
     2041 tgcaccatca gcacgttatc gaatcctttg ccacgtaagt ccgcatcttc atgacgacca
     2101 aagccagtaa agtagaacgg tttgtggtta atcaggaact gttggccctt cactgccact
     2161 gaccggatgc cgacgcgaag cgggtagata tcacactctg tctggctttt ggctgtgacg
     2221 cacagttcat agagataacc ttcacccggt tgccagaggt gcggattcac cacttgcaaa
     2281 gtcccgctag tgccttgtcc agttgcaacc acctgttgat ccgcatcacg cagttcaacg
     2341 ctgacatcac cattggccac cacctgccag tcaacagacg cgtggttaca gtcttgcgcg
     2401 acatgcgtca ccacggtgat atcgtccacc caggtgttcg gcgtggtgta gagcattacg
     2461 ctgcgatgga ttccggcata gttaaagaaa tcatggaagt aagactgctt tttcttgccg
     2521 ttttcgtcgg taatcaccat tcccggcggg atagtctgcc agttcagttc gttgttcaca
     2581 caaacggtga tacgtacact tttcccggca ataacatacg gcgtgacatc ggcttcaaat
     2641 ggcgtatagc cgccctgatg ctccatcact tcctgattat tgacccacac tttgccgtaa
     2701 tgagtgaccg catcgaaacg cagcacgata cgctggcctg cccaaccttt cggtataaag
     2761 acttcgcgct gataccagac gttgcccgca taattacgaa tatctgcatc ggcgaactga
     2821 tcgttaaaac tgcctggcac agcaattgcc cggctttctt gtaacgcgct ttcccaccaa
     2881 cgctgatcaa ttccacagtt ttcgcgatcc agactgaatg cccacaggcc gtcgagtttt
     2941 ttgatttcac gggttggggt ttctacagga cggaccatat ttataggtgt aatttatgta
     3001 gctgtaattt ttaccttatt aatatttttt acgctttgca ttcgacgact gaactcccaa
     3061 atatatgttt aactcgtctt ggtcgtttga atttttgttg ctgtgtttcc taatattttc
     3121 catcacctta aatatgttat tgtaatcctc aatgttgaac ttgcaattgg acacggcata
     3181 gttttccata gtcgtgtaaa acatggtatt ggctgcattg taatacatcc gactgagcgg
     3241 gtacggatct atgtgtttga gcagcctgtt caaaaactct gcatcgtcgc aaaacggaat
     3301 ttcgtcgaca gatcgtaacc acacaaaaaa ccaacacaca gttctaatga aaataaagaa
     3361 cttttattat cctcaggtct agctggaatt cagatctgtg attgtaaata aaatgtaatt
     3421 tacagtatag tattttaatt aatatacaaa tgatttgata ataattctta tttaactata
     3481 atatattgtg ttgggttgaa ttaaaggtcc cggcatcctc aaatgcataa tttcatagtc
     3541 ccccttgttg taagtgatgc gtatttctga atctttgtaa aatagcacac aagactccaa
     3601 cgcgtttggc gttttatttt cttgctcgac tctagtttat taggcctcta gagatccgta
     3661 tttataggtt tttttattac aaaactgtta cgaaaacagt aaaatactta tttatttgcg
     3721 agatggttat cattttaatt atctccatga tccaataacc tagaataaag ggcccgacct
     3781 ttaattcaac ccaacacaat atattatagt taaataagaa ttattatcaa atcatttgta
     3841 tattaattaa aatactatac tgtaaattac attttattta caatcacaga tccccgggac
     3901 tagtaataaa agatgtttat tttcattaga tgtgtgtgtt ggttttttgt gtctagtaat
     3961 aaaacctaga ggatcgatcc cccggatctg atcatggaga taattaaaat gataaccatc
     4021 tcgcaaataa ataagtattt tactgttttc gtaacagttt tgtaataaaa aaacctataa
     4081 atatcggatc catggctgaa accaagatta tctaccacat ggacgaggaa gagactccat
     4141 acctcgtaaa gctgccggtt gctccagaac gtgtcactct ggctgacttc aagaacgtcc
     4201 tgagcaaccg cccggttcac gcatacaaat tcttctttaa gtcaatggac caggactttg
     4261 gcgtagttaa ggaggaaatc ttcgacgata acgcaaagct gccatgcttt aacggccgtg
     4321 tagtatcttg gctggtttta gctgaaggtg cacacagcga cgctggctcc caaggcaccg
     4381 atagccacac tgatctgccg ccacctctcg aacgcaccgg cggtataggc gactctcgtc
     4441 cgccgagctt ccacccaaac gtagcttctt cgcgtgacgg tatggataat gaaaccggca
     4501 ccgaatctat ggtctctcac cgccgcgaac gtgcccgtcg ccgtaaccgt gaagaggccg
     4561 ctcgcaccaa cggtcaccca cgtggtgacc gtcgccgtga tgtaggcctg ccgcctgaca
     4621 gcgcttccac cgctctgtcc tctgaactgg aaagctctag cttcgtggat tctgacgaag
     4681 acggtagcac cagccgcctg agcagctcca ccgaacaatc cacttcttcc cgtctgatcc
     4741 gcaaacataa acgtcgccgt cgcaagcagc gcctccgcca ggctgaccgt gcgtcctctt
     4801 tctcctccat caccgactcc accatgagcc tgaacatcgt tactgtgacc ttgaacatgg
     4861 agcgtcacca tttcctgggc attagcatcg ttggccaaag caacgatcgc ggcgacggtg
     4921 gtatctatat aggctccatc atgaagggcg gcgccgttgc cgcggacggt cgcattgaac
     4981 cgggtgatat gctcctgcag gtgaacgatg tcaatttcga gaacatgagc aacgacgatg
     5041 cagttcgtgt tctgcgtgaa atcgttagcc agactggccc gatctctctg accgtagcta
     5101 agtgttggga cccgactcct cgttcctatt tcaccgttcc acgtgcagac ccggtacgcc
     5161 cgattgaccc ggcagcctgg ctgagccaca ctgctgctct gaccggtgca ctgccacgtt
     5221 acgaactcga agaagcaccg ctgactgtga agtccgatat gagcgctgta gttcgtgtaa
     5281 tgcaactgcc agattccggt ctcgaaatcc gtgaccgtat gtggctgaag atcactatcg
     5341 ctaacgccgt tatcggcgct gacgtagttg actggctcta cacccatgta gaaggcttta
     5401 aggagcgtcg tgaagcacgt aaatacgcat cctcgctgct taaacacggc tttctgcgcc
     5461 acaccgtaaa caagataacc ttctctgaac agtgctacta tgtgttcggc gacctctgta
     5521 gcaatctggc cactctgaac ctgaactccg gcagctccgg tacttctgac caggatacct
     5581 tagctccgct tcctcaccct gccgcaccgt ggccattagg ccagggctat ccatatcagt
     5641 acccgggtcc tccgccttgt ttcccgccgg cttaccagga cccaggtttt agctacggct
     5701 ctggttctac cggtagccag cagtccgagg gcagcaaatc ctctggctcc actcgctcta
     5761 gccgccgtgc tccaggccgt gagaaagaac gccgtgctgc cggtgctggc ggtagcggct
     5821 ctgaatccga tcacactgct ccttctggtg taggtagctc ctggcgtgag cgccctgctg
     5881 gtcagctgag ccgtggttcc agcccacgtt cccaagctag cgctactgct ccgggcttgc
     5941 cgccaccgca cccgactact aaggcatata ccgtagttgg tggtccgcca ggtggtcctc
     6001 cagttcgtga gctggccgcc gttcctcctg aactgaccgg ttcccgtcag agctttcaga
     6061 aagctatggg taacccgtgt gaattttttg ttgacatcat gtgataagct tgcggccgca
     6121 ctcgagcacc accaccacca ccactaacct aggtagctga gcgcatgcaa gctgatccgg
     6181 gttattagta catttattaa gcgctagatt ctgtgcgttg ttgatttaca gacaattgtt
     6241 gtacgtattt taataattca ttaaatttat aatctttagg gtggtatgtt agagcgaaaa
     6301 tcaaatgatt ttcagcgtct ttatatctga atttaaatat taaatcctca atagatttgt
     6361 aaaataggtt tcgattagtt tcaaacaagg gttgtttttc cgaaccgatg gctggactat
     6421 ctaatggatt ttcgctcaac gccacaaaac ttgccaaatc ttgtagcagc aatctagctt
     6481 tgtcgatatt cgtttgtgtt ttgttttgta ataaaggttc gacgtcgttc aaaatattat
     6541 gcgcttttgt atttctttca tcactgtcgt tagtgtacaa ttgactcgac gtaaacacgt
     6601 taaatagagc ttggacatat ttaacatcgg gcgtgttagc tttattaggc cgattatcgt
     6661 cgtcgtccca accctcgtcg ttagaagttg cttccgaaga cgattttgcc atagccacac
     6721 gacgcctatt aattgtgtcg gctaacacgt ccgcgatcaa atttgtagtt gagctttttg
     6781 gaattatttc tgattgcggg cgtttttggg cgggtttcaa tctaactgtg cccgatttta
     6841 attcagacaa cacgttagaa agcgatggtg caggcggtgg taacatttca gacggcaaat
     6901 ctactaatgg cggcggtggt ggagctgatg ataaatctac catcggtgga ggcgcaggcg
     6961 gggctggcgg cggaggcgga ggcggaggtg gtggcggtga tgcagacggc ggtttaggct
     7021 caaatgtctc tttaggcaac acagtcggca cctcaactat tgtactggtt tcgggcgccg
     7081 tttttggttt gaccggtctg agacgagtgc gatttttttc gtttctaata gcttccaaca
     7141 attgttgtct gtcgtctaaa ggtgcagcgg gttgaggttc cgtcggcatt ggtggagcgg
     7201 gcggcaattc agacatcgat ggtggtggtg gtggtggagg cgctggaatg ttaggcacgg
     7261 gagaaggtgg tggcggcggt gccgccggta taatttgttc tggtttagtt tgttcgcgca
     7321 cgattgtggg caccggcgca ggcgccgctg gctgcacaac ggaaggtcgt ctgcttcgag
     7381 gcagcgcttg gggtggtggc aattcaatat tataattgga atacaaatcg taaaaatctg
     7441 ctataagcat tgtaatttcg ctatcgttta ccgtgccgat atttaacaac cgctcaatgt
     7501 aagcaattgt attgtaaaga gattgtctca agctcggatc gatcccgcac gccgataaca
     7561 agccttttca tttttactac agcattgtag tggcgagaca cttcgctgtc gtcgaggttt
     7621 aaacgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     7681 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     7741 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     7801 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     7861 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     7921 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     7981 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     8041 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     8101 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     8161 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     8221 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     8281 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     8341 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     8401 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     8461 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     8521 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     8581 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     8641 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     8701 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     8761 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     8821 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     8881 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     8941 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     9001 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     9061 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     9121 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     9181 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     9241 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     9301 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     9361 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     9421 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     9481 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     9541 agtgccacct gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     9601 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     9661 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     9721 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     9781 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     9841 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     9901 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     9961 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttccc attcgccatt
    10021 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgcca
//
