LOCUS       sequence-52243-e         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Hilf RJ, Dutzler R
  TITLE     Structure of a potentially open state of a proton-activated 
            pentameric ligand-gated ion channel.
  JOURNAL   Nature. 2009 Jan 1. 457(7225):115-8.
  PUBMED    18987630
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Jan 1. 457(7225):115-8."
COMMENT     Sequence Label: pet26-MBP-ELIC
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    72..96
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             111..133
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     sig_peptide     141..206
                     /codon_start=1
                     /gene="pelB (fragment)"
                     /product="leader peptide for secretion"
                     /label=pelB signal sequence
                     /translation="MKYLLPTAAAGLLLLAAQPAMA"
     CDS             246..275
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga
       61 ctcactatag gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac
      121 tttaagaagg agatatacat atgaaatacc tgctgccgac cgctgctgct ggtctgctgc
      181 tcctcgctgc ccagccggcg atggccatgg atatcggaat taattcggat ccgaattcga
      241 gctcacatca ccatcaccat caccatcacc atcaccccat gaaaatcgaa gaaggtaaac
      301 tggtaatctg gattaacggc gataaaggct ataacggtct cgctgaagtc ggtaagaaat
      361 tcgagaaaga taccggaatt aaagtcaccg ttgagcatcc ggataaactg gaagagaaat
      421 tcccacaggt tgcggcaact ggcgatggcc ctgacattat cttctgggca cacgaccgct
      481 ttggtggcta cgctcaatct ggcctgttgg ctgaaatcac cccggacaaa gcgttccagg
      541 acaagctgta tccgtttacc tgggatgccg tacgttacaa cggcaagctg attgcttacc
      601 cgatcgctgt tgaagcgtta tcgctgattt ataacaaaga tctgctgccg aacccgccaa
      661 aaacctggga agagatcccg gcgctggata aagaactgaa agcgaaaggt aagagcgcgc
      721 tgatgttcaa cctgcaagaa ccgtacttca cctggccgct gattgctgct gacgggggtt
      781 atgcgttcaa gtatgaaaac ggcaagtacg acattaaaga cgtgggcgtg gataacgctg
      841 gcgcgaaagc gggtctga
//
