LOCUS       sequence-52246-e         605 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Watabe-Uchida M, Zhu L, Ogawa SK, Vamanrao A, Uchida N
  TITLE     Whole-brain mapping of direct inputs to midbrain dopamine neurons.
  JOURNAL   Neuron. 2012 Jun 7;74(5):858-73.
  PUBMED    22681690
REFERENCE   2  (bases 1 to 605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron."; 
            date: "2012-06-7"; volume: "74(5)"; pages: "858-73"
COMMENT     Sequence Label: pCA-FLEX
FEATURES             Location/Qualifiers
     source          1..605
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    32..166
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(56..75)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     110..129
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(488..507)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(569..591)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 agctgggtac cgggcccccc ctcgaggtcg agatccagac atgataagat acattgatga
       61 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
      121 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttgcta gcatgcgata
      181 acctcgtata gcatacatta tacgaagtaa ttctttgcac cattctaaag aatagtactg
      241 ataatttctg ggttaaggca aattacctcg tataggatac tttatacgaa gttatgtcga
      301 cgatatcaag cttagatcta ttacttcgta taatgtatgc tatacgaggt tattaagagg
      361 tttcatattg ctaatagtac ttacaatcca gctaccattc tgcataactt cgtataaagt
      421 atcctatacg aggtaatgaa ttcaaccgta cgtatcaaat caattctttg ccaaaatgat
      481 gagacagcac aataaccagc acgttgccca ggagctgtag gaaaaagaag aaggcatgaa
      541 catggttagc agaggctcta gagccgccgg tcacacgcca gaagccgaac cccgccctgc
      601 cccgt
//
