LOCUS       sequence-52251-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SIRT6
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    72..96
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             111..133
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             152..169
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             179..196
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga
       61 ctcactatag gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac
      121 tttaagaagg agatatacca tgggcagcag ccatcatcat catcatcaca gcagcggcct
      181 ggttccgcgt ggtagtccgg aggagctgga gcggaaggtg tgggaactgg cgaggctggt
      241 ctggcagtct tccagtgtgg tgttccacac gggtgccggc atcagcactg cctctggcat
      301 ccccgacttc aggggtcccc acggagtctg gaccatggag gagcgaggtc tggcccccaa
      361 gttcgacacc acctttgaga gcgcgcggcc cacgcagacc cacatggcgc tggtgcagct
      421 ggagcgcgtg ggcctcctcc gcttcctggt cagccagaac gtggacgggc tccatgtgcg
      481 ctcaggcttc cccagggaca aactggcaga gctccacggg aacatgtttg tggaagaatg
      541 tgccaagtgt aagacgcagt acgtccgaga cacagtcgtg ggcaccatgg gcctgaaggc
      601 cacgggccgg ctctgcaccg tggctaaggc aagggggctg cgagcctgca ggggagagct
      661 gagggacacc atcctagact gggaggactc cctgcccgac cgggacctgg cactcgccga
      721 tgaggccagc aggaacgccg acctgtccat cacgctgggt acatcgctgc agatccggcc
      781 cagcgggaac ctgccgctgg ctaccaagcg ccggggaggc cgcctggtca tcgtca
//
