LOCUS       sequence-52271-e         707 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Di Fiore B, Pines J
  TITLE     Emi1 is needed to couple DNA replication with mitosis but does not 
            regulate activation of the mitotic APC/C.
  JOURNAL   J Cell Biol. 2007 May 7;177(3):425-37.
  PUBMED    17485488
REFERENCE   2  (bases 1 to 707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2007-05-7"; volume: "177(3)"; pages: "425-37"
COMMENT     Sequence Label: pT7-Venus-Emi1 (914)
FEATURES             Location/Qualifiers
     source          1..707
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     213..234
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      276..285
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(314..333)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(315..333)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(378..581)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(407..427)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg
       61 ggcgaagcac tgcaggccgt agcccagggt ggtcacgagg gtgggccagg gcacgggcag
      121 cttgccggtg gtgcagatca gcttcagggt cagcttgccg taggtggcat cgccctcgcc
      181 ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg
      241 caccaccccg gtgaacagct cctcgccctt gctcaccatg gtggcgaccg gtagcgctag
      301 caagcttggg tctccctata gtgagtcgta ttaatttcga taagccagta agcagtgggt
      361 tctctagtta gccagagagc tctgcttata tagacctccc accgtacacg cctaccgccc
      421 atttgcgtca atggggcgga gttgttacga cattttggaa agtcccgttg attttggtgc
      481 caaaacaaac tcccattgac gtcaatgggg tggagacttg gaaatccccg tgagtcaaac
      541 cgctatccac gcccattgat gtactgccaa aaccgcatca ccatggtaat agcgatgact
      601 aatacgtaga tgtactgcca agtaggaaag tcccataagg tcatgtactg ggcataatgc
      661 caggcgggcc atttaccgtc attgacgtca atagggggcg tacttgg
//
