LOCUS       sequence-52277-e         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  TITLE     pGEX-KG-GST-YAP
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCMX-Gal4-TEAD1-D278K
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..74
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        55..73
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             106..546
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
ORIGIN
        1 tagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac
       61 gactcactat agggagaccc aagcttgaag caagcctcct gaaagatgaa gctactgtct
      121 tctatcgaac aagcatgcga tatttgccga cttaaaaagc tcaagtgctc caaagaaaaa
      181 ccgaagtgcg ccaagtgtct gaagaacaac tgggagtgtc gctactctcc caaaaccaaa
      241 aggtctccgc tgactagggc acatctgaca gaagtggaat caaggctaga aagactggaa
      301 cagctatttc tactgatttt tcctcgagaa gaccttgaca tgattttgaa aatggattct
      361 ttacaggata taaaagcatt gttaacagga ttatttgtac aagataatgt gaataaagat
      421 gccgtcacag atagattggc ttcagtggag actgatatgc ctctaacatt gagacagcat
      481 agaataagtg cgacatcatc atcggaagag agtagtaaca aaggtcaaag acagttgact
      541 gtatcgccgg aattcgaaag gatgagtgac tctgcagata agccaattga caatgatgca
      601 gaaggggtct ggagccccga catcgagcaa agctttcagg aggccctggc tatctatcca
      661 ccatgtggga ggaggaaaat catcttatca gacgaaggca aaatgtatgg taggaatgaa
      721 ttgatagcca gatacatcaa actcaggaca ggcaagacga ggaccagaaa acaggtgtct
      781 agtcacattc aggttcttgc cagaaggaaa tctcgtgatt ttcattccaa gctaaaggat
      841 cagactgcaa aggataaggc cctgcagcac atggcggcca tgtcctcagc
//
