LOCUS       sequence-52295-e         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Korneel Hens, Jean-Daniel Feuz, Alina Isakova, Antonina Lagovitina, 
            Andreas Massouras, Julien Bryois, Patrick Callaerts, Susan E. 
            Celniker, Bart Deplancke
  TITLE     Automated protein-DNA interaction screening of Drosophila regulatory
            elements
  JOURNAL   Nat Methods. 2011 Oct 30;8(12):1065-70.
  PUBMED    22037703
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-10-30"; volume: "8(12)"; pages: "1065-70"
COMMENT     Sequence Label: KHBD00682
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    74..98
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             234..260
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
ORIGIN
        1 aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg ataagcaatg
       61 ctttcttata atgccaactt tgtacaagaa agttggcaaa tagggattaa ctgccgccag
      121 accaactctt ccgccggtcc gggcgaaaac gggctccgca tagctgtgcc tatagccctt
      181 ggccatttgg gttagtggct tgcccgaatt gccaccggaa gaagcaccgc tgccaccacc
      241 accaccacca ccaccaccac ttccgcctac atcaacagat tgtatgggcg tggctgctgg
      301 tgagggatat cgctccatgt ggccgcgacc cgaacccgtt gttgatggat tcttggtttc
      361 cacgctctgc aggaattcgc gtgagtgggc gcgtccaccg ggtctttcac gctccctctc
      421 atgccgatag ggatcccggg actggtctcg attgtagtcc cgatctctat cccgctctct
      481 gtcacgcacg ctgcgctcca agtgcctggg tggctccggt tcccattcgc cgcgcgatct
      541 ggctcgggat cggtttagat cacgtggatt ggacttttcg ctgatcacca ccggcggttc
      601 ctcctcctcg ctccactgct cgtacgtgct gggtggctcg acggaaccgc gacgccggcg
      661 cgatgacgac cgatacggat cccggtgcgg tgtgtcgcgc tcatccacgt ccggatagcg
      721 atttaccgcc ggacgctgtt ccatg
//
