LOCUS       sequence-52328-e         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Ito-Harashima S, McCusker JH
  TITLE     Positive and negative selection LYS5MX gene replacement cassettes 
            for use in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2004 Jan 15;21(1):53-61.
  PUBMED    14745782
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "2004-01-15"; volume: "21(1)"; pages: "53-61"
COMMENT     Sequence Label: pSA40
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(24..57)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      complement(120..317)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 actagtggat ctgatatcac ctaataactt cgtatagcat acattatacg aagttatatt
       61 aagggttctc gagagctcgt tttcgacact ggatggcggc gttagtatcg aatcgacagc
      121 agtatagcga ccagcattca catacgattg acgcatgata ttactttctg cgcacttaac
      181 ttcgcatctg ggcagatgat gtcgaggcga aaaaaaatat aaatcacgct aacatttgat
      241 taaaatagaa caactacaat ataaaaaaac tatacaaatg acaagttctt gaaaacaaga
      301 atctttttat tgtcagtact gattataaac catcattttc gatgaaataa tcaatcaacg
      361 tagataagct gatattatat aattttggtc tgttcgtgtt gattttatca ctgatggact
      421 ttggcataca gatagtgaca atttcgttat tgaaccattg agaatggaaa atcaatggaa
      481 cttcatccag agttatgcac atagaagctc cctcagccgg aaaaaagctg atagcgccaa
      541 aatctattag tgacaagtct gtgttaaggc cagttccagt aaattttgta tacgactcct
      601 tcaaggacca taagtaagta aatattgtgc atggatcaga cgctttcagt aaaccgttaa
      661 attctctttc actaaaaact tctttaaata gctccaactc ttccctcccg ccataattgc
      721 acggagaagc gatatcaatt ccgacatcct ggtattcatc tgtacttaca cattttacga
      781 ggaacatagc tacatattgt tcaccgatgg tcatgctaaa tggaagaaaa cgattgttgt
      841 ctaagaatgg cttaccgaag ctgcccttgt caaatttcag ctcttgaaaa tttaagcccg
      901 ttactat
//
