LOCUS       sequence-52332-e         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Vorachek-Warren MK, McCusker JH
  TITLE     DsdA (D-serine deaminase): a new heterologous MX cassette for gene 
            disruption and selection in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2004 Jan 30;21(2):163-71.
  PUBMED    14755641
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "2004-01-30"; volume: "21(2)"; pages: "163-71"
COMMENT     Sequence Label: pMKV006
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(76..109)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      complement(172..369)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 gactcactat agggcgaatt ggagctccac cgcggtggcg gccgcatagg ccactagtgg
       61 atctgatatc acctaataac ttcgtatagc atacattata cgaagttata ttaagggttc
      121 tcgagagctc gttttcgaca ctggatggcg gcgttagtat cgaatcgaca gcagtatagc
      181 gaccagcatt cacatacgat tgacgcatga tattactttc tgcgcactta acttcgcatc
      241 tgggcagatg atgtcgaggc gaaaaaaaat ataaatcacg ctaacatttg attaaaatag
      301 aacaactaca atataaaaaa actatacaaa tgacaagttc ttgaaaacaa gaatcttttt
      361 attgtcagta ctgattaacg gccttttgcc agatattgat tcatctcttc ttccggcacc
      421 attccacctc ccgtcgccca caccagatga gtggtattac gcagttgttc tgcgctgaaa
      481 ccgtgcatct gttggtaact tactgatgca cacacgcgct gaggtccggc catacccgcc
      541 agtgccgaag gttcaagacg aataccttct tcctgcgcca gccagccaag catgtcatac
      601 atggtttgat cgctaagggt atagaagcca tccagcagac gctccattgc ccgcccgaca
      661 aagcctgatg cgcgaccaac tgcaaggcca tccgctgcgg taaggttgtc gataccaata
      721 tcctgaacag aaatctgatc gtgtaatcct gtatggacgc ctaacaacat acaaggggag
      781 tgcgttggtt cggcaaaaaa gcagtgaaca tgatcgccaa acgccagttt aagcccgaat
      841 gcgacgccac caggaccacc gcca
//
