LOCUS       sequence-52335-e         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  TITLE     Chin Lab P85 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti4/V5-DEST-P85(K550_K562dup)
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    12..30
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    71..95
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             119..142
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tgatagagat ctccctatca gtgatagaga tcgtcgacta gtccagtgtg gtggaattct
       61 gcagatatca acaagtttgt acaaaaaagc aggctccgcg gccgccccct tcaccatgga
      121 ctataaggac gatgatgaca aatccatggc catgagtgct gaggggtacc agtacagagc
      181 gctgtatgat tataaaaagg aaagagaaga agatattgac ttgcacttgg gtgacatatt
      241 gactgtgaat aaagggtcct tagtagctct tggattcagt gatggacagg aagccaggcc
      301 tgaagaaatt ggctggttaa atggctataa tgaaaccaca ggggaaaggg gggactttcc
      361 gggaacttat gtagaatata ttggaaggaa aaaaatctcg cctcccacac caaagccccg
      421 gccacctcgg cctcttcctg ttgcaccagg ttcttcgaaa actgaagcag atgttgaaca
      481 acaagctttg actctcccgg atcttgcaga gcagtttgcc cctcctgaca ttgccccgcc
      541 tcttcttatc aagctcgtgg aagccattga aaagaaaggt ctggaatgtt caactctata
      601 cagaacacag agctccagca acctggcaga attacgacag cttcttgatt gtgatacacc
      661 ctccgtggac ttggaaatga tcgatgtgca cgttttggct gacgctttca aacgctatct
      721 cctggactta ccaaatcctg tcattccagc agccgtttac agtgaaatga t
//
