LOCUS       sequence-52336-e         626 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 626)
  TITLE     Chin Lab P85 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti4/V5-DEST-P85(KS459del)
FEATURES             Location/Qualifiers
     source          1..626
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    22..46
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             70..93
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ggtggaattc tgcagatatc aacaagtttg tacaaaaaag caggctccgc ggccgccccc
       61 ttcaccatgg actataagga cgatgatgac aaatccatgg ccatgagtgc tgaggggtac
      121 cagtacagag cgctgtatga ttataaaaag gaaagagaag aagatattga cttgcacttg
      181 ggtgacatat tgactgtgaa taaagggtcc ttagtagctc ttggattcag tgatggacag
      241 gaagccaggc ctgaagaaat tggctggtta aatggctata atgaaaccac aggggaaagg
      301 ggggactttc cgggaactta tgtagaatat attggaagga aaaaaatctc gcctcccaca
      361 ccaaagcccc ggccacctcg gcctcttcct gttgcaccag gttcttcgaa aactgaagca
      421 gatgttgaac aacaagcttt gactctcccg gatcttgcag agcagtttgc ccctcctgac
      481 attgccccgc ctcttcttat caagctcgtg gaagccattg aaaagaaagg tctggaatgt
      541 tcaactctat acagaacaca gagctccagc aacctggcag aattacgaca gcttcttgat
      601 tgtgatacac cctccgtgga cttgga
//
