LOCUS       sequence-52337-e         720 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Korneel Hens, Jean-Daniel Feuz, Alina Isakova, Antonina Lagovitina, 
            Andreas Massouras, Julien Bryois, Patrick Callaerts, Susan E. 
            Celniker, Bart Deplancke
  TITLE     Automated protein-DNA interaction screening of Drosophila regulatory
            elements
  JOURNAL   Nat Methods. 2011 Oct 30;8(12):1065-70.
  PUBMED    22037703
REFERENCE   2  (bases 1 to 720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-10-30"; volume: "8(12)"; pages: "1065-70"
COMMENT     Sequence Label: KHBD00712
FEATURES             Location/Qualifiers
     source          1..720
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    20..44
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             83..91
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 gcaatgcttt cttataatgc caactttgta caagaaagtt ggcaactgcg ctcctggatc
       61 cttggcctcg ccaggtggcg ggacctcctt aatggactcc accttggcca caattttaag
      121 agctggaccc agcttcatgc ccatagcgtt caccaaatgc ttctccttga gcaacagaag
      181 cgcttggccg tcgatctcct gctgtataaa gtcgtccacg tagtcctgac aaccaggcag
      241 ttctcgaatg aagttgctga catcgtccac actccagctg ctgattggcg gacgatctgt
      301 tccattcact tccgaagact tgggcgccgc gccagaagtg actacagaca gtggtgcaag
      361 agttggaagt gcaactgaca atggcgatcc tagaggcatt gcaagaggcg agggcgcaga
      421 aatcgccgct tggactggca gtgacattgg aggtacttct gttgaggctc ccaaaatagg
      481 aaacgattct gaaagctttg gggtggcctc tgtctgcatc ttctcctcag ccatggcttc
      541 atccagcctg tccaccaatg ccatggcgtc caccccaact ataccacctg tccccaggcc
      601 gttcgtctct cctgatccaa ctccaccgat gccgttcttt gcctgcctcg agcatcctgg
      661 cgaacagtag cgcttccgtt tcagctttgc tttgtgctcc atctttccac actgctcgca
//
