LOCUS       sequence-52338-e         631 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 631)
  TITLE     Chin Lab P85 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti4/V5-DEST-P85(E439del)
FEATURES             Location/Qualifiers
     source          1..631
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    20..44
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             68..91
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tggaattctg cagatatcaa caagtttgta caaaaaagca ggctccgcgg ccgccccctt
       61 caccatggac tataaggacg atgatgacaa atccatggcc atgagtgctg aggggtacca
      121 gtacagagcg ctgtatgatt ataaaaagga aagagaagaa gatattgact tgcacttggg
      181 tgacatattg actgtgaata aagggtcctt agtagctctt ggattcagtg atggacagga
      241 agccaggcct gaagaaattg gctggttaaa tggctataat gaaaccacag gggaaagggg
      301 ggactttccg ggaacttatg tagaatatat tggaaggaaa aaaatctcgc ctcccacacc
      361 aaagccccgg ccacctcggc ctcttcctgt tgcaccaggt tcttcgaaaa ctgaagcaga
      421 tgttgaacaa caagctttga ctctcccgga tcttgcagag cagtttgccc ctcctgacat
      481 tgccccgcct cttcttatca agctcgtgga agccattgaa aagaaaggtc tggaatgttc
      541 aactctatac agaacacaga gctccagcaa cctggcagaa ttacgacagc ttcttgattg
      601 tgatacaccc tccgtggact tggaaatgat c
//
